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The Use of Short-Term Tests in the Isolation and Identification of Chemical Mutagens in Complex Mixtures

  • Chapter
Chemical Mutagens

Abstract

The feasibility of using short-term mutagenicity assays to isolate and identify the potential biohazards) of complex materials is being examined by use of various coupled chemical and biological approaches. Such research has usually involved a preliminary chemical characterization and preparation for bioassay, followed by testing for bioactivity (generally the mutagenicity test for Salmonella histidine reversion described by Ames et al. p(1). Subsequent fractionation procedures to further characterize the mutagens present are carried out, with the bioassay being used as a tool to follow the activity and guide the separations. The mutagenicity tests are intended to function as (1) predictors of profound long-range health effects such as mutagenesis and/or carcinogenesis; (2) a mechanism to rapidly isolate and identify a hazardous biological agent in a complex mixture; and (3) a measure of biological activity, correlating baseline data with changes in experimental (or environmental) conditions and, in the case of actual industrial effluents or streams, with changes in process conditions. With this combined chemical fractionation and short-term assay approach, the investigator can accumulate information on the actual compounds responsible for the biological effect. Thus, the mutagenicity tests will also function as (4) an aid in identifying the specific hazardous compounds involved and in establishing priorities for more definitive chemical analysis and monitoring along with further validative testing in comparative systems, including whole-animal testing, for mutagenesis and carcinogenesis.

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References

  1. B. N. Ames, J. McCann, and E. Yamasaki, Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test, Mutat. Res. 31, 347–364 (1975).

    CAS  Google Scholar 

  2. I. B. Rubin, M. R. Guerin, A. A. Hardigree, and J. L. Epler, Fractionation of synthetic crude oils from coal for biological testing, Environ. Res. 12, 358–365 (1976).

    Article  CAS  Google Scholar 

  3. M. R. Guerin and J. L. Epler, Determining emissions measurements needs for an emerging industry—advanced fossil fuels utilization, Presented at the First Conference on Determining Fugitive Emissions Measurements Needs, Hartford, Connecticut, May 17–19, 1976, EPA Technology Series, EPA-600/2-76-246.

    Google Scholar 

  4. J. L. Epler, J. A. Young, A. A. Hardigree, T. K. Tao, M. R. Guerin, I. B. Rubin, C.-h. Ho, and B. R. Clark, Analytical and biological assessment of test materials from the synthetic fuel technologies. I. Mutagenicity of crude oils determined by the Salmonella typhimurium/ microsomal activation system, Mutat. Res. 57, 265–276 (1978).

    CAS  Google Scholar 

  5. J. L. Epler, M. R. Guerin, and T. K. Rao, Evaluation of feasibility of mutagenic testing of shale oil products and effluents, Environ. Health Perspect. 30, 179–184 (1979).

    Article  CAS  Google Scholar 

  6. T. K. Rao, J. L Epler, I. B. Rubin, and M. R. Guerin, Environmental mutagenesis of energy-related effluents, Genetics 83, s60 (1976).

    Google Scholar 

  7. J. L. Epler, A. A. Hardigree, J. A. Young, and T. K. Rao, Feasibility of application of mutagenicity testing to aqueous environmental effluents, Mutat. Res. 53, 88 (1978) (abstract).

    Google Scholar 

  8. T. K. Rao, A. A. Hardigree, J. A. Young, and J. L. Epler, Correlation of mutagenic activity of energy-related effluents with organic constituents, Mutat. Res. 53, 88–89 (1978) (abstract).

    Google Scholar 

  9. J. L. Epler, B. R. Clark, C.-h. Ho, M. R. Guerin, and T. K. Rao, Short-term bioassay of complex organic mixtures. Part I: Chemistry, and Part II: Mutagenicity testing, Presented at the Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures, Williamsburg, Virginia, February 21–23, 1978, pp. 249–268 and 271–289, EPA-600/9-78-027, September 1978.

    Google Scholar 

  10. J. L. Epler, F. W. Larimer, T. K. Rao, C. E. Nix, and T. Ho, Energy-related pollutants in the environment: The use of short-term tests for mutagenicity in the isolation and identification of biohazards, Presented at Higher Plant Systems as Monitors of Environmental Mutagens, a workshop co-sponsored by the National Institute of Environmental Health Sciences and the Department of Energy, Marineland, Florida, January 16–18, 1978, Environ. Health Perspect. 27, 11–20 (1978).

    Article  CAS  Google Scholar 

  11. B. R. Clark, I. B. Rubin, C.-H. Ho, M. R. Guerin, J. L. Epler, and A. A. Hardigree, Testing for health hazards in coal liquids: Development of a method for the systematic assessment of biological and environmental effects of fossil fuel conversion materials, in: Coal Processing Technology, Vol 3, technical manual published by American Institute of Chemical Engineers (1977).

    Google Scholar 

  12. D. L. Klass, Synthetic crude oil from shale and coal, Chem. Technol., August, 499–510 (1975).

    Google Scholar 

  13. L. D. Kier, E. Yamasaki, and B. N. Ames, Detection of mutagenic activity in cigarette smoke condensates, Proc. Natl. Acad. Sci. U.S.A. 71, 4159–4163 (1974).

    Article  CAS  Google Scholar 

  14. R. E. Kouri, K. Brandt, R. Sosnowski, W. F. Benedict, and L. M. Schechtman, Metabolic activation of cigarette smoke condensate materials to their mutagenic forms in vitro, Presented at the Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures, Williamsburg, Virginia, February 21–23, 1978, pp. 495–512, EPA-600/9-78-027, September 1978.

    Google Scholar 

  15. J. J. Hutton and C. Hackney, Metabolism of cigarette smoke condensates by human and rat homogenates to form mutagens detectable by Salmonella typhimurium TAI538, Cancer Res. 35, 2461 (1975).

    CAS  Google Scholar 

  16. E. Yamasaki and B. N. Ames, Concentration of mutagens from urine by absorption with the nonpolar resin XAD-2: Cigarette smokers have mutagenic urine, Proc. Natl. Acad. Sei. U.S.A. 74, 3555–3559 (1977).

    Article  CAS  Google Scholar 

  17. A. A. Hardigree and J. L. Epler, Mutagenicity of plant flavonols in microbial systems, Mutat. Res. 53, 89 (1978) (abstract).

    Google Scholar 

  18. L. F. Bjeldanes and G. W. Chang, Mutagenic activity of quercetin and related compounds, Science 197, 577–578 (1977).

    Article  CAS  Google Scholar 

  19. A. J. Vithayathil, B. Commoner, S. Nair, and P. Madyastha, Isolation of mutagens from bacterial nutrients containing beef extract, /. Toxciol. Environ. Health 4, 189–201 (1978).

    Article  CAS  Google Scholar 

  20. M. Nagao, T. Yahagi, T. Kawachi, Y. Seino, M. Honda, N. Matsukura, T. Sugimura, K. Wakabayashi, K. Tsuji, and T. Kosuge, Mutagens in foods, and especially pyrolysis products of protein, in: Progress in Genetic Toxciology (D. Scott, B. A. Bridges, and F. H. Sobels, eds.), pp. 259–264, Elsevier/North-Holland, New York (1977).

    Google Scholar 

  21. B. N. Ames, H. O. Kämmen, and E. Yamasaki, Hair dyes are mutagenic: Identification of a variety of mutagenic ingredients, Proc. Natl. Acad. Sei. U.S.A. 72, 2423–2427 (1975).

    Article  CAS  Google Scholar 

  22. W. Thilly and T. Skopek, Soot, and soot components: In vitro chronic exposure studies, Presentation for Subcommittee on Environmental Mutagenesis, Bethesda, Maryland, January 1978 (personal communication).

    Google Scholar 

  23. T. R. Skopek, H. L. Liber, J. J. Krolewski, and W. G. Thilly, Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker, Proc. Natl. Acad. Sei. U.S.A. 75, 410–414 (1978).

    Article  CAS  Google Scholar 

  24. D. Neubert, Nature and levels of chemical environmental mutagens, industrial exposure and population at risk, in: Progress in Genetic Toxicology (D. Scott, B. A. Bridges, and F. H. Sobels, eds.), pp. 95–115, Elsevier/North-Holland, New York (1977).

    Google Scholar 

  25. L. Tomatis, The IARC program on the evaluation of the carcinogenic risk of chemicals to man, Ann. N.Y. Acad. Sei. 271, 396–409 (1976).

    Article  CAS  Google Scholar 

  26. C. E. Chrisp, G. L. Fisher, and J. E. Lammert, Mutagenicity of filtrates from respirable coal fly ash, Science 199, 73–75 (1978).

    Article  CAS  Google Scholar 

  27. J. N. Pitts, Jr., D. Grosjeru, T. M. Mischke, V. F. Simmon, and D. Poole, Mutagenic activity of airborne particulate organic pollutants, Toxicol. Lett. 1, 65–70 (1977).

    Article  CAS  Google Scholar 

  28. J. M. Daisey, I. Hawryluk, T. J. Kneip, and F. H. Mukai, Mutagenic activity in organic fractions of airborne particulate matter, Presented at the Conference on Carbonaceous Particles in the Atmosphere, Berkeley, California, March 20–22, 1978.

    Google Scholar 

  29. B. Commoner, P. Madyastha, A. Bronsdon, and A.J. Vithayathil, Environmental mutagens in urban air particulates, J. Toxicol. Environ. Health 4, 59–77 (1978).

    Article  CAS  Google Scholar 

  30. R. Talcot and E. Wei, Airborne mutagens bioassayed in Salmonella typhimurium, J. Natl. Cancer Inst. 58, 449–451 (1977).

    Google Scholar 

  31. H. Tokiwa, K. Morita, H. Takeyoshi, K. Takahashi, and Y. Ohnishi, Detection of mutagenic activity in particulate air pollutants, Mutat. Res. 48, 237–248 (1977).

    Article  CAS  Google Scholar 

  32. W. Dehnen, N. Pitz, and R. Tomingas, The mutagenicity of airborne particulate pollutants, Cancer Lett. 4, 5–12 (1977).

    Article  Google Scholar 

  33. K. Teranishi, K. Hamada, and H. Watanabe, Mutagenicity in Salmonella typhimurium mutants of the benzene-soluble organic matter derived from airborne particulate matter and its five fractions, Mutat. Res. 56, 273–280 (1978).

    Article  CAS  Google Scholar 

  34. M. R. Guerin, B. R. Clark, C.-h. Ho, J. L. Epler, and T. K. Rao, Short-Term Bioassay of Complex Organic Mixtures: Part I, Chemistry, Presented at the Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mix-tures, Williamsburg, Virginia, February 21–23, 1978, pp. 248–268, EPA-600/9-78-027, September 1978.

    Google Scholar 

  35. Y. Wang, Mutagens in automobile exhaust, Presented at the Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures, Williamsburg, Virginia, February 21–23, 1978, p. 587, EPA 600/9-78-027, Sept. 1978.

    Google Scholar 

  36. B. Commoner, Carcinogens in the environment, Chem. Technol. 7 (2), 76–82 (1977).

    CAS  Google Scholar 

  37. B. Commoner and A. Vithayathil, The analysis of urine samples as a means of detecting exposure to environmental organic carcinogens, Presented at Biological Specimen Collection International Workshop, Luxembourg, April 18–22, 1977.

    Google Scholar 

  38. R. C. vonBorstel and M. M. Shahin, Personal communication.

    Google Scholar 

  39. W. H. Griest, T. K. Rao, and J. L. Epler, Unpublished observations.

    Google Scholar 

  40. T. K. Rao, J. L. Epler, J. J. Schmidt-Collerus, L. Leffler, and M. R. Guerin, Biological monitoring of oil shale products and effluents using short-term genetic analyses, Presented at the Oil Shale Sampling, Analyses and Quality Assurance Symposium, Denver, Colorado, March 26–28, 1979.

    Google Scholar 

  41. Federal Register, February 6, 1978, Proposed “Criteria for Classification of Solid Waste Disposal Facilities,” Office of Solid Waste, U.S. EPA, Washington, D.C., Vol. 43, No. 25.

    Google Scholar 

  42. IERL-RTP Procedures Manual: Level 1 Environmental Assessment, EPA Technology Series, EPA-600/2-76-160, June 1976.

    Google Scholar 

  43. R. K. Ham, M. A. Anderson, R. Stanforth, and R. Stegmann, The development of a leaching test for industrial wastes, Presented at the Fourth Annual Research Symposium on Land Disposal of Hazardous Waste, San Antonio, Texas, March 6–8, 1978.

    Google Scholar 

  44. B. Commoner, Chemical carcinogens in the environment, in: Identification and Analysis of Organic Pollutants in Water ( L. H. Keith, ed), Ann Arbor Science, Ann Arbor, Michigan (1977).

    Google Scholar 

  45. B. Commoner, Chemical carcinogens in the environment, Abstracts: First Chemical Congress of the North American Continent, Mexico City, Mexico, November 30–December 5, 1975.

    Google Scholar 

  46. J. M. Parry, D. J. Tweats, and M. A. J. Al-Mossawi, Monitoring the marine environment for mutagens, Nature (London) 364, 538–540 (1976).

    Article  Google Scholar 

  47. J. F. Payne, Oil spills: Effects of petroleum on marine organisms, Science 196, 10 (1977).

    Article  CAS  Google Scholar 

  48. U. Rannug and C. Ramel, The mutagenicity of waste products from the vinyl chloride industries, Mutat. Res. 38, 113 (1976).

    Google Scholar 

  49. W. S. Barnes and E. J. Klekowski, Jr., Testing the environment for dispersed mutagens: Use of plant bioconcentrators coupled with microbial mutagen assays, Presented at Higher Plant Systems as Monitors of Environmental Mutagens, a workshop co-sponsored by the National Institute of Environmental Health Sciences and the Department of Energy, Marineland, Florida, January 16–18, 1978, Environ. Health Perspect. 27, 61–67 (1978).

    CAS  Google Scholar 

  50. S. J. Mule, M. J. Bastos, D. Jukofsky, and E. Saffer, Routine identification of drugs of abuse in human urine. II. Development and application of the XAD-2 resin column method, J. Chromatogr. 63, 289–301 (1971).

    Article  CAS  Google Scholar 

  51. C. Osterroht, Development of a method for the extraction and determination of non-polar, dissolved organic substances in sea water, /. Chromatogr. 101, 289–398 (1974).

    Article  CAS  Google Scholar 

  52. D. C. Kennedy, Treatment of effluent from manufacture of chlorinated pesticides with a synthetic, polymeric absorbent, amberlite XAD-4, Environ. Sci. Technol. 7, 138 (1973).

    Article  CAS  Google Scholar 

  53. J. A. Leenheer and E. W. D. Huffman, Jr., Classification of organic solutes in water by using macroreticular resins,/. Res. U.S. Geol. Surv. 4 (6), 737–751 (1976).

    CAS  Google Scholar 

  54. T. H. Connor and M. S. Legator, A method for separation and analysis of body fluids in rodents and humans for mutagenic components using Salmonella typhimurium as the indicator of mutagenicity, Mutat. Res. 53, 71–72 (1978) (abstract).

    Google Scholar 

  55. M. S. Legator, L. Truong, and T. H. Connors, Analysis of body fluids including alkylation of macromolecules for detection of mutagenic agents, in: Chemical Mutagens: Principles and Methods for Their Detection, Vol. 5 (A. Hollaender and F. J. de Serres, eds.), pp. 1–23, Plenum Press, New York (1978).

    Google Scholar 

  56. V. Leoni, G. Puecetti, R. J. Colombo, and A. M. D’Ovidio, The use of Tenax for the extraction of pesticides and polychlorinated biphenyls from water. II. Tests with artificially polluted and natural waters,/. Chromatogr. 125, 399–407 (1976).

    Article  CAS  Google Scholar 

  57. A. K. Burnham, G. V. Calder, J. S. Fritz, G. A. Junk, H.J. Svec, and R. Willis, Identification and estimation of neutral organic contaminants in potable water, Anal. Chem. 44, 139— 142 (1972).

    Google Scholar 

  58. R. R. Pelroy and M. R. Petersen, Mutagenicity of Shale Oil Components, Presented at the Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures, Williamsburg, Virginia, February 21–23, 1978, pp. 461– 475, EPA-600/9-78-027, September 1978.

    Google Scholar 

  59. W. E. Durston and B. N. Ames, A simple method for the detection of mutagens in urine: Studies with the carcinogen 2-acetylaminofluorene, Proc. Natl. Acad. Sci. U.S.A. 71, 737–741 (1974).

    Article  CAS  Google Scholar 

  60. B. Commoner, A. J. Vithayathil, and J. I. Henery, Detection of metabolic carcinogen intermediates in urine of carcinogen-fed rats by means of bacterial mutagenesis, Nature (London) 249, 850–852 (1974).

    Article  CAS  Google Scholar 

  61. A. J. Varghese, P. C. Land, and W. R. Bruce, Properties of a mutagen isolated from human feces, Abstracts: Environmental Mutagen Society 9th Annual Meeting, San Francisco, California, March 9–13, 1978.

    Google Scholar 

  62. J. C. Loper, D. R. Lang, C. C. Smith, R. S. Schoeny, F. C. Kopfler, and R. G. Tardiff, In vitro mutagenesis and carcinogenesis testing of residual organics in drinking water, in: Proceedings Second International Symposium on Aquatic Pollutants, Pergamon Press, Oxford, England (1979).

    Google Scholar 

  63. J. C. Loper and D. R. Lang, Mutagenic, carcinogenic, and toxic effects of residual organics in drinking water, Presented at the Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures, Williamsburg, Virginia, February 21–23, 1978, pp. 513–528, EPA-600/9-78-027, September 1978.

    Google Scholar 

  64. F. C. Kopfler, W. E. Coleman, R. G. Melton, R. G. Tardiff, S. C. Lynch, and J. K. Smith, Extraction and identification of organic micropollutants—reverse osmosis method, Ann. N.Y. Acad. Sci. 298, 20–30 (1977).

    Article  CAS  Google Scholar 

  65. F. A. Junk, J. J. Richard, M. D. Grieser, D. Witiak, J. L. Witiak, M. D. Arguello, R. Vick, H. J. Svec, J. S. Fritz, and G. V. Calder, Use of macroreticular resins in the analysis of water foF trace organic contaminants, J. Chromatogr. 99, 745–762 (1974).

    Article  CAS  Google Scholar 

  66. C. Chriswell, B. A. Glatz, J. S. Fritz, and H. J. Svec, Mutagenic analysis of drinking water samples, Presented at the Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures, Williamsburg, Virginia, February 21–23, 1928, pp. 477–494, EPA-600/9-78-027, September 1978.

    Google Scholar 

  67. V. F. Simmon, K. Kauhanen, and R. G. Tardiff, Mutagenic activity of chemicals identified in drinking water, in: Progress in Genetic Toxicology (D. Scott, B. A. Bridges, and F. H. Sobels, eds.), pp. 249 - 258, Elsevier/North-Holland, New York (1977).

    Google Scholar 

  68. J. L. Epler, F. W Larimer, C. E. Nix, T. Ho, and T. K. Rao, Comparative mutagenesis of test material from the synthetic fuel technologies, in: Progress in Genetic Toxicology (D. Scott, B. A. Bridges, and F. H. Sobels, eds), pp. 275–284, Elsevier/North-Holland, New York (1977).

    Google Scholar 

  69. J. Maxild, M. Andersen, and P. Kiel, Mutagenicity of fume particles from metal arc welding on stainless steel in the Salmonella/microsome test, Mutat. Res. 56, 235–243 (1978).

    Article  CAS  Google Scholar 

  70. R. Tomingas, G. Voltmer, and R. Bednarik, Direct fluorometric analysis of aromatic polycyclic hydrocarbons on thin layer chromatograms, Sci. Total Environ. 1, 261–267 (1977).

    Google Scholar 

  71. E. L. Wynder and G. Wright, A study of tobacco carcinogenesis. I. The primary fractions, Cancer 10, 255–271 (1957).

    Article  CAS  Google Scholar 

  72. E. L. Wynder and D. Hoffman, The epidermis and the respiratory tract as bioassay systems in tobacco carcinogenesis, Br. J. Cancer 24, 574–587 (1970).

    Article  CAS  Google Scholar 

  73. F. G. Bock, A. P. Swain, and R. L. Stedman, Bioassay of major fractions of cigarette smoke condensate by an accelerated technic, Cancer Res. 29, 584–587 (1969).

    CAS  Google Scholar 

  74. F. G. Bock, A. P. Swain, and R. L. Stedman, Carcinogenesis assay of subfractions of cigarette smoke condensate prepared by solvent-solvent separation of the neutral fraction, /. Natl. Cancer Inst. 49, 477–483 (1972).

    CAS  Google Scholar 

  75. A. P. Swain, J. E. Cooper, and R. L. Stedman, Large-scale fractionation of cigarette smoke condensate for chemical and biologic investigations, Cancer Res. 29, 579–583 (1969).

    CAS  Google Scholar 

  76. J. H. Bell, S. Ireland, and A. W. Spears, Identification of aromatic ketones in cigarette smoke condensate, Anal. Chem. 41, 310–313 (1969).

    Article  CAS  Google Scholar 

  77. J. H. Bell, S. Ireland, and A. W. Spears, Identification of aromatic ketones in cigarette smoke condensate, Anal. Chem. 41, 310–313 (1969).

    Article  CAS  Google Scholar 

  78. T. K. Rao, J. A. Young, A. A. Hardigree, W. Winton, and J. L. Epler, Analytical and biological analyses of test material from the synthetic fuel technologies. II. Extended genetic and biological studies with mutagenic fractions, Mutat. Res. 54, 185–191 (1978).

    CAS  Google Scholar 

  79. D. S. Farrier, J. E. Virgona, T. E. Phillips, and R. E. Poulson, Environmental research for in situ oil shale processing, Presented at and published in the Proceedings of the Eleventh Oil Shale Symposium, Golden, Colorado, April 12–14, 1977.

    Google Scholar 

  80. M. R. Petersen, J. S. Fruchter, and J. C. Laul, Characterization of substances in products, effluents, and wastes from synthetic fuel production tests, Quarterly Report, Battelle Pacific Northwest Laboratories, BNWL-2131/UC-48, September 1976.

    Google Scholar 

  81. A. R. Jones, M. R. Guerin, and B. R. Clark, Preparative-scale liquid chromatographic fractionation of crude oils derived from coal and shale, Anal. Chem. 49, 1766–1771 (1977).

    Article  CAS  Google Scholar 

  82. J. L. Epler, J. A. Young, T. K. Rao, M. R. Guerin, C.-h. Ho, and B. R. Clark, Isolation and identification of mutagenic components from synthetic fuels, Abstracts: Environmental Mutagen Society 9th Annual Meeting, San Francisco, California, March 9–13, 1978.

    Google Scholar 

  83. M. R. Guerin, C.-h. Ho, B. R. Clark, J. L. Epler, and T. K. Rao, Separation of mutagenic components in synthetic crudes. Presented to the Division of Environmental Chemistry, American Chemical Society, Anaheim, California, March 12–17, 1978.

    Google Scholar 

  84. M. R. Guerin. J, L. Epler, W. H. Griest, B. R. Clark, and T. K. Rao, Polycyclic aromatic hydrocarbons from fossil fuel conversion processes, in: Carcinogenesis, Vol. 3, Polynuclear Aromatic Hydrocarbons (P. W. Jones and R. J. Freudenthal, eds.), pp. 21–33, Raven Press, New York (1978).

    Google Scholar 

  85. W. H. Griest, G. Olerich, J. L. Epler, and T. K. Rao, Characterization of multialkylated polycyclic aromatic hydrocarbons in energy-related materials, Presented at the Third International Symposium on Polynuclear Aromatic Hydrocarbons, Battelle Columbus Laboratories, Columbus, Ohio, October 25–28, 1978.

    Google Scholar 

  86. R. F. Severson, M. E. Snook, F. J. Akin, and O. T. Chortyk, The correlation of biological activity with PAHs of tobacco smoke fractions, in: Carcinogenesis, Vol. 3, Polynuclear Aromatic Hydrocarbons (P. W. Jones and R. J. Freudenthal, eds.), pp. 115–130, Raven Press, New York (1978).

    Google Scholar 

  87. T. Sugimura, M. Nagao, T. Kawachi, M. Honda, T. Yahagi, Y. Seino, S. Sato, N. Matsukura, T. Matsushima, A. Shirai, M. Sawamura, and H. Matsumoto, Mutagens-foods, Cold Spring Harbor Conf. Cell Proliferation 4, 710 (1979).

    Google Scholar 

  88. A. A. Hardigree and J. L. Epler, Comparative mutagenesis of plant flavonoids in microbial systems, Mutat. Res. 58, 231–239 (1978).

    Article  CAS  Google Scholar 

  89. J. P. Brown, R. J. Brown, and G. W. Roehm, The application of short-term microbial mutagenicity tests in the identification and development of non-toxic, non-absorbable food additives, in: Progress in Genetic Toxicology (D. Scott, B. A. Bridges, and F. H. Sobels, eds.), pp. 185–190, Elsevier/North-Holland, New York (1977).

    Google Scholar 

  90. B. N. Ames, F. D. Lee, and W. E. Durston, An improved bacterial test system for the detection and classification of mutagens and carcinogens: Salmonella typhimurium/Mpopolysaccharide/frameshift mutations, Proc. Natl. Acad. Sci. U.S.A. 70, 782–786 (1973).

    Article  CAS  Google Scholar 

  91. M. H. L. Green, W. J. Muriel, and B. A. Bridges, Use of a simplified fluctuation test to detect low levels of mutagens, Mutat. Res. 38, 33–42 (1976).

    CAS  Google Scholar 

  92. G. R. Mohn and J. Ellenberger, The use of Escherichia coli K12/343/113 (X) as a multipurpose indicator strain in various mutagenicity testing procedures, in: Handbook of Mutagenicity Test Procedures (B. j. Kilbey, M. Legator, W. Nichols, and C. Ramel, eds.), pp. 95–118, Elsevier/North-Holland, Amsterdam (1977).

    Google Scholar 

  93. T. Kada, K. Titikawa, and Y. Sadaie, In vitro and host-mediated “rec-assay” procedures for screening chemical mutagens; and phloxine, a mutagenic red dye, Mutat. Res. 16, 165–174 (1972).

    Article  CAS  Google Scholar 

  94. E. E. Slater, M. D. Anderson, and H. S. Rosenkranz, Rapid detection of mutagens and carcinogens, Cancer Res. 31, 970–973 (1971).

    CAS  Google Scholar 

  95. F. K. Zimmermann, Procedures used in the induction of mitotic recombination and mutation in the yeast Saccharomyces cerevisiae, Mutat. Res. 31, 71–86 (1975).

    CAS  Google Scholar 

  96. D. M. DeMarini, The mutagenicity of cigarette smoke condensate in Saccharomyces cerevisiae, Presented at the 8th Annual Meeting of the Environmental Mutagen Society, Colorado Springs, Colorado, February 13–17, 1977, Mutat. Res. 53, 84 (1978).

    Google Scholar 

  97. F. W. Larimer, D. W. Ramey, W. Lijinsky, and J. L. Epler, Mutagenicity of methylated N- nitrosopiperidines in Saccharomyces cerevisiae, Mutat. Res. 57, 155–161 (1978).

    Article  CAS  Google Scholar 

  98. T. Ho, S. Tipton, and J. L. Epler, Unpublished observations.

    Google Scholar 

  99. S. Wolff, Sister chromatid exchange, Annu. Rev. Genet. 11, 183–201 (1977).

    Article  CAS  Google Scholar 

  100. A. W Hsie, J. P. O’Neill, J. R. San Sebastian, D. B. Couch, P. A. Brimer, W. N. C. Sun, J. C. Fuscoe, N. L. Forbes, R. Machanoff, J. C. Riddle, and M. H. Hsie, Mutagenicity of carcinogens: Study of 101 individual agents and 3 subfractions of a crude synthetic oil in a quantitative mammalian cell gene mutation system, Presented at the Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures, Williamsburg, Virginia, February 21–23, 1978, pp. 291–315, EPA 600/9-78–027, Sept. 1978.

    Google Scholar 

  101. C. E. Nix and B. S. Brewen, The role of Drosophila in chemical mutagenesis testing, Presented at the Symposium on Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures, Williamsburg, Virginia, February 21–23, 1978, pp. 112 — 123, EPA 600/9-78-027, Sept. 1978.

    Google Scholar 

  102. W. M. Generoso and L. R. Russell, Integrated chemical and biological testing of synthetic oils and effluents—mammalian genetics, Proceedings of Symposium on Potential Health and Environmental Effects of Synthetic Fossil Fuel Technologies, Gatlinburg, Tennessee, September 25 - 28, 1978.

    Google Scholar 

  103. J. M. Holland, Integrated chemical and biological testing of synthetic oils and effluents— carcinogenesis testing, Proceedings of Symposium on Potential Health and Environmental Effects of Synthetic Fossil Fuel Technologies, Gatlinburg, Tennessee, September 25 - 28, 1978.

    Google Scholar 

  104. J. Ashby and J. A. Styles, Does carcinogenic potency correlate with mutagenic potency in the Ames assay? Nature (London) 271, 452–455 (1978).

    Article  CAS  Google Scholar 

  105. J. A. Young, T. K. Rao, C. E. Weeks, T. J. Slaga, and J. L. Epler, Effect of the co- carcinogen benzo(e)pyrene on microsome mediated chemical mutagenesis in Salmonella typhimurium, Abstracts: Environmental Mutagen Society 9th Annual Meeting, San Francisco, California, March 9–13, 1978.

    Google Scholar 

  106. K. Umezawa, A. Shiri, T. Matsushima, and T. Sugimura, Comutagenic effect of norharman and harman with 2-acetylaminofluorene derivatives: Salmonella typhimurium/microsomal enzymes/carcinogens, Proc. Natl. Acad. Sci. U.S.A. 75, 928–930 (1978).

    Article  CAS  Google Scholar 

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  1. Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, 37830, USA

    J. L. Epler

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  1. J. L. Epler
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  1. National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA

    Frederick J. de Serres

  2. Associated Universities, Inc., Washington, D. C., USA

    Alexander Hollaender

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© 1980 Plenum Press, New York

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Epler, J.L. (1980). The Use of Short-Term Tests in the Isolation and Identification of Chemical Mutagens in Complex Mixtures. In: de Serres, F.J., Hollaender, A. (eds) Chemical Mutagens. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3072-1_9

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  • DOI: https://doi.org/10.1007/978-1-4613-3072-1_9

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