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Determining the DNA-Modifying Activity of Chemicals Using DNA-Polymerase-Deficient Escherichia coli

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Chemical Mutagens

Abstract

Microbial mutagenicity assay procedures have received considerable attention recently because of the realization that many chemical carcinogens derive their biological activity from their ability to react chemically with cellular DNA. Since mutagens also derive their capacity to induce hereditary changes from their reactivity with cellular DNA, it was logical that mutagenicity testing methods should be evaluated for their potential to detect carcinogens as mutagens. Indeed, the Salmonella mutagenicity assay procedure recently developed by Ames et al. p(1) has shown a remarkable ability to detect carcinogens as microbial mutagens. p(2,3) Furthermore, a number of investigators have actually demonstrated a relationship between specific mutagenic activity and carcinogenic potential p(14,62) (however, see also references 63–67).

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References

  1. B. N. Ames, J. McCann, and E. Yamasaki, Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test, Mutat. Res. 31, 347–364 (1975).

    CAS  Google Scholar 

  2. J. McCann, E. Choi, E. Yamasaki, and B. N. Ames, Detection of carcinogens as mutagens in the Salmonella/microsome test: Assay of 300 chemicals, Proc. Natl. Acad. Sei. U.S.A. 72, 5135–5139 (1975).

    Article  CAS  Google Scholar 

  3. M. Meselson and K. Russell, Comparisons of carcinogenic and mutagenic potency, in Origins of Human Cancer, Book C, pp. 1473–1481, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1977).

    Google Scholar 

  4. H. S. Rosenkranz and L. A. Poirier, An evaluation of the mutagenicity and DNA-modifying activity in microbial systems of carcinogens and non-carcinogens, J. Natl. Cancer Inst. 62, 873–892 (1979).

    CAS  Google Scholar 

  5. H. S. Rosenkranz, B. Gutter, and W. T. Speck, Mutagenicity and DNA`modifying activity: A comparison of two microbial assays, Mutat. Res. 41, 61–70 (1976).

    Article  CAS  Google Scholar 

  6. H. S. Rosenkranz, W. T. Speck, and B. Gutter, Microbial assay procedures: Experience with two systems, in: In Vitro Activation in Mutagenesis Testing (F. J. de Serres, J. R. Fouts, J. R. Bond, and R. M. Philpot, eds.), pp. 337–363, Elsevier/North-Holland, Amsterdam (1976).

    Google Scholar 

  7. H. S. Rosenkranz, E. C. McCoy, M. Anders, W. T. Speck, and D. Bickers, The use of microbial assay systems in the detection of environmental mutagens in complex mixtures, in: Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures (M. D. Waters, S. Nesnow, J. L. Huisingh; S. S. Sandhu, and L. Claxton, eds.), U.S. Environmental Protection Agency, EPA-600-9-78-027, pp. 3–42 ( 1978 ); Plenum Press, New York (1979).

    Google Scholar 

  8. H. S. Rosenkranz, Aspects of microbiology in cancer research, Annu. Rev. Microbiol. 27, 383–401 (1973).

    Article  CAS  Google Scholar 

  9. E. E. Slater, M. D. Anderson, and H. S. Rosenkranz, Rapid detection of mutagens and carcinogens, Cancer Res. 31, 970–973 (1971).

    CAS  Google Scholar 

  10. P. DeLucia and J. Cairns, Isolation of an E. coli strain with a mutation affecting DNA polymerase, Nature (London) 224, 1164–1166 (1969).

    Article  CAS  Google Scholar 

  11. J. Gross and M. Gross, Genetic analysis of E. coli strain with a mutation affecting DNA polymerase, Nature (London) 224, 1166–1168 (1969).

    Article  CAS  Google Scholar 

  12. K. P. Mullinix and H. S. Rosenkranz, Recovery from N-hydroxyurethan-induced death,/. Bacteriol. 105, 565–572 (1971).

    CAS  Google Scholar 

  13. C. D. Town, K. C. Smith, and H. S. Kaplan, DNA polymerase required for rapid repair of x-ray-induced DNA strand breaks in vivo, Science 172, 851–854 (1971).

    CAS  Google Scholar 

  14. 15.M. C. Paterson, J. M. Boyle, and R. B. Setlow, Ultraviolet- and x-ray induced responses of a deoxyribonucleic acid polymerase-deficient mutant of Escherichia coli, J. Bacteriol. 107, 61–67 (1971).

    CAS  Google Scholar 

  15. H. S. Rosenkranz, R. Hjorth, and H. S. Carr, Studies with hydroxyurea: The biologic and metabolic properties of formamidoxime, Biochim. Biophys. Acta 232, 48–60 (1971).

    CAS  Google Scholar 

  16. E. B. Winshell and H. S. Rosenkranz, Nalidixic acid and the metabolism of Escherichia coli,]. Bacteriol. 104, 1168–1175 (1970).

    CAS  Google Scholar 

  17. K. Massafi, H. S. Carr, and H. S. Rosenkranz, Hydroxyurea and cell death, Isr. J. Med. Sei. 8, 559–561 (1972).

    CAS  Google Scholar 

  18. H. S. Rosenkranz, Miracil D: Inhibition of deoxyribonucleic acid polymerase-deficient Escherichia coli, Antimicrob. Agents Chemother. 3, 530–531 (1973).

    CAS  Google Scholar 

  19. Y. Ishii and S. Kondo, Comparative analysis of deletion and base-change mutabilities of Escherichia coli B strains differing in DNA repair capacity (Wild-type, uvrA -, pol A x-, rec A-) by various mutagens, Mutat. Res. 27, 27–44 (1975).

    Article  CAS  Google Scholar 

  20. F. S. Southwick, H. S. Carr, G. A. Carden III, R. M. D’Alisa, and H. S. Rosenkranz, Effects of acridine orange on the growth of Escherichia coli, J. Bacteriol. 110, 439–441 (1972).

    CAS  Google Scholar 

  21. W. L. Fangman and M. Russel, X-irradiation sensitivity in Escherichia coli defective in DNA replication, Mol. Gen. Genet. 110, 332–347 (1971).

    Article  CAS  Google Scholar 

  22. M. Monk, M. Peacey, and J. D. Gross, Repair of damage induced by ultraviolet light in DNA polymerase-defective Escherichia coli cells, J. Mol. Biol. 58, 623–630 (1971).

    Article  CAS  Google Scholar 

  23. B. N. Ames, W. E. Durston, E. Yamasaki, and F. D. Lee, Carcinogens are mutagens: A simple test system combining liver homogenates for activation and bacteria for detection, Proc. Natl. Acad. Sci. U.S.A. 70, 2281–2285 (1973).

    Article  CAS  Google Scholar 

  24. C. N. Frantz and H. V. Mailing, The quantitative microsomal mutagenesis assay method, Mutat. Res. 31, 365–380 (1975).

    CAS  Google Scholar 

  25. H. Nishioka, Supersensitive DNA repair test-liquid for confirming DNA damagicity of mutagens, Abstracts of the 2nd International Conference on Environmental Mutagens, Edinburg, p. 121 (1977).

    Google Scholar 

  26. S. S. Cohen and H. D. Barner, Studies on unbalanced growth in Escherichia coli, Proc. Natl. Acad. Sei. U.S.A. 40, 885–893 (1954).

    Article  CAS  Google Scholar 

  27. H. S. Rosenkranz, A. J. Garro, J. A. Levy, and H. S. Carr, Studies with hydroxyurea. I. The reversible inhibition of bacterial DNA synthesis and the effect of hydroxyurea on the bactericidal action of streptomycin, Biochim. Biophys. Acta 114, 501–515 (1966).

    CAS  Google Scholar 

  28. H. S. Rosenkranz, M. Bitoon, and R. M. Schmidt, Biological and metabolic effects of nitrosomethylurea and nitrosomethylurethan, J. Natl. Cancer Inst. 41, 1099–1109 (1968).

    CAS  Google Scholar 

  29. V. F. Simmon, In vitro mutagenicity assays of chemical carcinogens and related compounds with Salmonella typhimurium, J. Natl. Cancer Inst. 62, 893–899 (1979).

    Google Scholar 

  30. H. S. Rosenkranz, H. S. Carr, and C. Morgan, Unusual growth properties of a bacterial strain lacking DNA polymerase, Biochem. Biophys. Res. Commun. 44, 546–549 (1971).

    Article  CAS  Google Scholar 

  31. H. S. Rosenkranz, H. S. Carr, and C. Morgan, Unusual growth properties of a bacterial strain lacking DNA polymerase, Biochem. Biophys. Res. Commun. 44, 546–549 (1971).

    Article  CAS  Google Scholar 

  32. H. S. Rosenkranz, Preferential effect of dichlorvos (Vapona) on bacteria deficient in DNA polymerase, Cancer Res. 33, 458–459 (1973).

    CAS  Google Scholar 

  33. E. T. Glaz, Preferential inhibition of DNA polymerase I deficient E. coli by dianhy- drohexitols, tilorone and metronidazole, Xlth Int. Cancer Congr. (Florence) Abstr. 2, 132 (1974).

    Google Scholar 

  34. E. T. Glaz, Proceedings of a Conference of the Chemotherapy Section, Hungarian Pharmacological Society, Siklos, Hungary, 22–28 (1974).

    Google Scholar 

  35. H. S. Rosenkranz and M. D. Anderson, Identification and action of environmental carcinogens,/. Isr. Biochem. Microbiol. Assoc. 1, 70–84 (1973).

    Google Scholar 

  36. H. S. Rosenkranz and H. S. Carr, Possible hazards in use of gentian violet, Br. Med. J. 3, 702–703 (1971).

    Article  CAS  Google Scholar 

  37. H. S. Rosenkranz, Sodium hypochlorite and sodium perborate: Preferential inhibitors of DNA polymerase-deficient bacteria, Mutat. Res. 21, 171–174 (1973).

    Article  CAS  Google Scholar 

  38. H. S. Rosenkranz and H. S. Carr, Silver sulfadiazine: Effect on the growth and metabolism of bacteria, Antimicrob. Agents Chemother. 2, 367–372 (1972).

    CAS  Google Scholar 

  39. R. M. D’Alisa, G. A. Carden III, H. S. Carr, and H. S. Rosenkranz, “Reversion” of DNA polymerase-deficient Escherichia coli, Mol. Gen. Genet. 110, 23–26 (1971).

    Google Scholar 

  40. D. S. Longnecker, T. J. Curphey, S. T. James, D. S. Daniel, and N. J. Jacobs, Trial of a bacterial screening system for rapid detection of mutagens and carcinogens, Cancer Res. 34, 1658–1663 (1974).

    CAS  Google Scholar 

  41. M. Nagao and T. Sugimura, Sensitivity of repair-deficient mutants and similar mutants to 4- nitroquinoline 1-oxide, 4-nitropyridine 1-oxide, and their derivatives, Cancer Res. 32, 2369– 2374 (1972).

    Google Scholar 

  42. E. R. Fluck, L. A. Poirier, and H. W. Ruelius, Evaluation of a DNA polymerase-deficient mutant of E. coli for the rapid detection of carcinogens, Chem.-Biol. Interact. 15, 219–231 (1976).

    Article  CAS  Google Scholar 

  43. H. S. Rosenkranz and H. S. Carr, Hydrazine antidepressants and isoniazid: Potential carcinogens, Lancet 1, 1354–1355 (1971).

    Article  CAS  Google Scholar 

  44. H. S. Rosenkranz, E. C. McCoy, L. Biuso, and W. T. Speck, Short-term microbiol assays in the assessment of carcinogenic risk, in: The Use of Alternatives in Drug Research (A. N. Rowan and C. J. Stratmann, eds.), pp. 103–145, MacMillan, London (1979).

    Google Scholar 

  45. H. S. Rosenkranz, B. Gutter, and W. T. Speck, Mutagenicity and DNA-modifying activity: A comparison of two microbial assays, Mutat. Res. 41, 61–70 (1976).

    Article  CAS  Google Scholar 

  46. H. S. Rosenkranz, and A. B. Stein, Picrolonic acid: A novel frameshift mutagen, Mutat. Res. 28, 127–129 (1975).

    Article  CAS  Google Scholar 

  47. H. S. Rosenkranz and T. J. Wlodkowski, Mutagenicity of ethylene chlorohydrin—a degradation product present in foodstuffs exposed to ethylene oxide, J. Agnc. Food Chem. 22, 407– 409 (1974).

    Google Scholar 

  48. H. S. Rosenkranz, Hydroxylamine-O-sulfonic acid: In vitro and possible in vivo reaction with DNA, Chem.-Biol. Interact. 7, 195–204 (1973).

    Article  CAS  Google Scholar 

  49. H. S. Rosenkranz, Formaldehyde as a possible carcinogen, Bull. Environ. Contamin. Toxicol. 8, 242–244 (1972).

    Article  CAS  Google Scholar 

  50. H. S. Rosenkranz, Mutagenicity of halogenated alkanes and their derivatives, Environ. Health Perspect. 21, 79–84 (1977).

    CAS  Google Scholar 

  51. S. Rosenkranz, H S. Carr, and H. S. Rosenkranz, 2-Haloethanols: Mutagenicity and reactivity with DNA, Mutat. Res. 26, 367–370 (1974).

    Article  CAS  Google Scholar 

  52. T. J. Wlodkowski and H. S. Rosenkranz, Mutagenicity of sodium hypochlorite for Salmonella typhimurium, Mutat. Res. 31, 39–42 (1975).

    CAS  Google Scholar 

  53. T. J. Wlodkowski. W. T. Speck, and H. S. Rosenkranz, Genetic effects of povidone-iodine,J. Pharm. Sei. 64, 1235–1237 (1975).

    Article  CAS  Google Scholar 

  54. H. Brem, A. B. Stein, and H. S. Rosenkranz, The mutagenicity and DNA-modifying effects of haloalkanes, Cancer Res. 34, 2576–2579 (1974).

    CAS  Google Scholar 

  55. E. C. McCoy, L. Burrows, and H. S. Rosenkranz, Genetic activity of allyl chloride, Mutat. Res. 57, 11–15 (1978).

    CAS  Google Scholar 

  56. H. S. Rosenkranz, Genetic activity of 1,2-dibromo-3-chloropropane, a widely used fumigant, Bull. Environ. Contam. Toxicol. 14, 8–12 (1975).

    Article  CAS  Google Scholar 

  57. H. S. Rosenkranz and F. S. Southwick, Preferential inhibition by chloroquine and quinacrine of E. coli deficient in DNA polymerase, Environ. Mutat. Soc. Nezuslett. 5, 38 (1971).

    Google Scholar 

  58. H. S. Rosenkranz, in: Cancer Testing Technology and Saccharin, U.S. Congress, Office of Technology Assessment (Superintendent of Documents, U.S. Government Printing Office, 052-003-00471-2), pp. 100–103 (1977).

    Google Scholar 

  59. H. S. Rosenkranz, in: Simple correlation and multiple regression among leaf characteristics, smoke components, and biological responses of bright tobaccos, Technical Bulletin No. 1551, Agricultural Research Service, U.S. Department of Agriculture (1977).

    Google Scholar 

  60. M. D. Anderson and H. S. Rosenkranz, Greenhouse fungicide—Environmental carcinogen?, Henry Ford Hosp. Med. J. 22, 35–40 (1974).

    CAS  Google Scholar 

  61. B.N. Ames and K. Hooper, Does carcinogenic potency correlate with mutagenic specificity in the Ames assay? A reply, Nature (London) 274, 19–20 (1978).

    Article  CAS  Google Scholar 

  62. M. M. Coombs, A. M. Kissonerghis, and J. A. Allan, Evaluation of the mutagenicity of compounds of known carcinogenicity belonging to the benz(a)anthracene, chrysene, and cyclopenta(a)phenanthrene series using Ames' test, Cancer Res. 36, 452–459 (1976).

    Google Scholar 

  63. J. Ashby and J. A. Styles, Does carcinogenic potency correlate with mutagenic potency in the Ames' assay?, Nature (London) 271, 452–455 (1978).

    Article  CAS  Google Scholar 

  64. J. Ashby and J. A. Styles, Does carcinogenic potency correlate with mutagenic potency in the Ames' assay?, Nature (London) 271, 452–455 (1978).

    Article  CAS  Google Scholar 

  65. J. Ashby and J. A. Styles, Does carcinogenic potency correlate with mutagenic potency in the Ames' assay?, Nature (London) 271, 452–455 (1978).

    Article  CAS  Google Scholar 

  66. J. Ashby and J. A. Styles, Does carcinogenic potency correlate with mutagenic potency in the Ames' assay?, Nature (London) 271, 452–455 (1978).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, New York Medical College, Valhalla, New York, 10595, USA

    Herbert S. Rosenkranz & Zev Leifer

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  1. Herbert S. Rosenkranz
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  2. Zev Leifer
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Editors and Affiliations

  1. National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA

    Frederick J. de Serres

  2. Associated Universities, Inc., Washington, D. C., USA

    Alexander Hollaender

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© 1980 Plenum Press, New York

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Rosenkranz, H.S., Leifer, Z. (1980). Determining the DNA-Modifying Activity of Chemicals Using DNA-Polymerase-Deficient Escherichia coli . In: de Serres, F.J., Hollaender, A. (eds) Chemical Mutagens. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3072-1_5

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  • DOI: https://doi.org/10.1007/978-1-4613-3072-1_5

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