Abstract
Populations of polyadenylated messenger RNA (mRNA) sequences can conveniently be characterised by studying the kinetics of hybridisation between the mRNA population and a cDNA copy population which is usually synthesised by using reverse transcriptase.1,2 The most common method is to anneal unlabelled mRNA with radioactively labelled cDNA, and to measure how much of the latter has become duplexed at different times by treating successive samples of the annealing mixture with nuclease S1. A description of the reaction and its analysis is given in references 1–4.
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References
J.O. Bishop, J.F. Morton, M. Rosbash and M. Richardson, Three abundance classes in HeLa cell mRNA. Nature 250: 199 (1974).
G,D. Bimie, E. MacPhail, B.D.Young, M.J.Getz and J.Paul, The diversity of the mRNA population in growing Friend cells. Cell Differentiation 3; 221 (1974).
N.D. Hastie and J.O. Bishop, The expression of three abundance classes of mRNA in mouse tissues. Cell 9: (1976).
J.O. Bishop, J. Beckman, M.S. Campo, N.D. Hastie, M.Izquierdo and S. Perlman, DNA-RNA hybridisation. Phil. Trans. Roy. Soc. B 272: 147 (1975).
L.M. Hereford and M. Rosbash, Number and distribution of polyadenylated RNA sequences in yeast. Cell 10L 453 (1977).
B. Levy W and B. J. McCarthy, Messenger RNA complexity in Drosophila melanogaster. Biochemistry, 14: 2440 (1975).
M. Izquierdo and J.O. Bishop, An analysis of cytoplasmic RNA populations in Drosophila melanogaster, Oregon R. Biochemical Genetics (1979)
P.Z. O’Farrell, H.M. Goodman and P.H. O’Farrell, High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12: 1133 (1977).
B.M. Paterson and J.O. Bishop, Changes in the mRNA population of chick myoblasts during myogenesis in vitro. Cell 12: 751 (1977).
N.D. Hastie and W.A. Held, Analysis of mRNA populations by cDNA- mRNA hybrid-mediated inhibition of cell-free protein synthesis. Proc. Nat. Acad. Sci., U.S. 75: 1217 (1978).
H.R.B. Pelham and J. Jackson, An efficient mRNA-dependent translation system from reticulocyte lysates. Ear. J. Biochem. 67: 247 (1976).
D.J. Finnegan, G.M. Rubin, M.W.Yoimg and D.S. Hogness, Repeated gene families in Drosophila melanogaster. Cold Spring Harbor Symposia Quant. Biol. 142: 1053 (1978).
S.S.Potter, W.J. Brorein, P.Dunsmuir and G.M. Rubin, Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell 175: 415 (1979).
E. Strobel, P. Dunsmuir and G.M. Rubin, Polymorphisms in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell 17: 429 (1979).
J.O. Bishop, A DM sequence cleaved by restriction endonuclease R. Eco RI in only one strand. J.Mol.Biol. 128: 545 (1979).
P.C. Wensink, B.J. Finnegan, J.E. Bonelson and B.S. Hogness, A system for mapping BNA sequences in the chromosomes of Drosophila melanogaster. Cell 3: 315 (197U).
M.E. Akman, B.B. Roberts, G.P. Richards and M. Ashbumer, Drosophila: the genetics of two major larval proteins. Cell 13: 215 (1978).
H. Ursprung and J. Leone, Alcohol dehydrogenase: a polymorphism in Drosophila melanogaster. J. Exper. Zool. 110:147 (1965).
E.M. Southern, Betection of specific BNA sequences among BNA fragments separated by electrophoresis. J.Mol.Biol. 98: 503 (1975).
C.K. Klukas and I.B. Bawid, Characterization and mapping of mitochondrial ribosomal RNA and mitochondrial BNA in Brosophila melanogaster. Cell 9: 6l5 (1976).
B.M. Paterson, B.E. Roberts and E.L. Kuff, Structural gene identification and mapping by BNA-mRNA hybrid-arrested cell-free translation. Proc. Nat. Acad. Sci., U.S, 74: 4370 (1977).
M. Ashbumer and J.J. Bonner, The induction of gene activity in Drosophila by heat shock. Cell 17: 214 I (1979).
B.J. Bower, Ph.B. Thesis, University of Edinburgh (l979).
J.T. Lis, L. Prestidge and B.S. Hogness, A novel arrangement of tandemly repeated genes at a major heat shock site in Drosophila. Cell 14: 901 (1978).
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© 1980 Plenum Press, New York
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Bishop, J.O., Clissold, P.M., Davis, J.A., Mason, P. (1980). Analysis and Resolution of mRNA Populations. In: Leaver, C.J. (eds) Genome Organization and Expression in Plants. NATO Advanced Study Institutes Series, vol 29. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3051-6_9
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DOI: https://doi.org/10.1007/978-1-4613-3051-6_9
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