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Expression of the Cauliflower Mosaic Virus Genome in Turnips (Brassica Rapa)

  • S. H. Howell
  • J. T. Odell
  • K. R. Dudley
Part of the NATO Advanced Study Institutes Series book series (volume 29)

Abstract

Cauliflower mosaic virus (CaMV) DNA is a possible vehicle for introducing foreign DNA into plant cells. CaMV is one of the few plant viruses that has a double-stranded DNA genome and the DNA, isolated from the virus, is infective.1 CaMV would probably have to be considered as a “nonintegrating” vehicle, since there is no evidence as yet that the viral DNA integrates into the host plant genome.1 However, the possibility for using nonintegrating viral DNAs as molecular vehicles in animal cells has been made dramatically apparent this past year through the experiments of Mulligan et al. who used SV40 DNA to introduce and express rabbit β-globin cDNA in monkey cells.2 These authors replaced a viral structural gene in SV40 DNA with foreign DNA thereby placing the inserted DNA element under viral genome control.

Keywords

Coat Protein Leaf Disc Infected Leaf Alkaline Sucrose Gradient Host Plant Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1).
    R. J. Shepherd, DNA Plant Viruses, Ann. Rev. Plant Physiol. 30: 405 (1979).CrossRefGoogle Scholar
  2. 2).
    R. C. Mulligan, B. H. Howard, and P. Berg. Synthesis of rabbit β-globin, Nature 277, 108 (1979).PubMedCrossRefGoogle Scholar
  3. 3).
    J. T. Odell and S. H. Howell, in preparation.Google Scholar
  4. 4).
    B. M. Paterson, B. E. Roberts and E. L. Kuff. Structural gene identification and mapping, Proc. Nat. Acad. Sci. U.S. 74: 4370 (1977).CrossRefGoogle Scholar
  5. 5).
    S. H. Howell and R. Hull. Replication of cauliflower mosaic virus and transcription of its genome. Virology 86: 468 (1978).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • S. H. Howell
    • 1
  • J. T. Odell
    • 1
  • K. R. Dudley
    • 1
  1. 1.Biology Department C016University of California, San DiegoLa JollaUSA

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