Concurrent Extraction and GC-MS Assay of Endogenous TP, T, IAA, 5HT and 5HIAA in Single Rat Brain Samples

  • F. Artigas
  • E. Gelpí


The indoleamine Serotonin (5-hydroxytryptamine) is presently considered as one of the few established central neurotransmitters, its metabolism having been related to physiological functions of the body as well as several psychiatric disorders, such as schizophrenia and depression. Consequently, various analytical methods have been developed for serotonin (5HT) as well as for its precursor tryptophan (TP) and its acidic metabolite, 5-hydroxyindole- -3-acetic acid (5HIAA) in brain tissue (1–3).


Deuterated Standard Propionic Anhydride Mass Fragmentographic Boron Trichloride Mass Fragmentograms 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1).
    W.D. Denkla and H.K. Dewey, J. Lab. Clin. Med., 1967, 69, 160.Google Scholar
  2. 2).
    G. Curzon and A.R. Green, Br. J. Pharmacol., 1970, 39, 653.Google Scholar
  3. 3).
    O. Beck, F.A. Wiesel and G. Sedvall, J. Chromatogr., 1977, 134, 407.CrossRefGoogle Scholar
  4. 4).
    W.R. Martin and J.W. Sloan, Psychopharmacologia, 1970, 18, 231.Google Scholar
  5. 5).
    S.R. Philips, D.A. Durden and A.A. Boulton, Can. J. Biochem., 1974, 52, 447.Google Scholar
  6. 6).
    J.M. Saavedra and J. Axelrod, J. Pharmacol. Exp. Ther., 1972, 182, 363.Google Scholar
  7. 7).
    S.R. Snodgrass and A.S. Horn, J. Neurochem., 1973, 21, 687.Google Scholar
  8. 8).
    W.R. Martin, J.W. Sloan, S.T. Christian and T.H. Clements, Psychopharmacologia, 1972, 24, 331.CrossRefGoogle Scholar
  9. 9).
    J.J. Warsh, D.D. Godse, H.C. Stancer, P.W. Chan and D.V. Coscina Biochem. Med., 1977,18 10.Google Scholar
  10. 10).
    J.J. Warsh, P.W. Chan, D.D. Godse, D.V. Coscina and H.C. Stancer J. Neurochem., 1977,.29, 955.CrossRefGoogle Scholar
  11. 11).
    J. Segura, J. Veiguela and E. Gelpí, in preparation.Google Scholar
  12. 12).
    F. Artigas, E. Gelpí, M. Prudencio, J.A. Alonso and J. Ballart, Anal. Chem., 1977, 49, 543.Google Scholar
  13. 13).
    F. Artigas and E. Gelpi, Anal. Biochem., in press.Google Scholar
  14. 14).
    E. Gelpi, E. Peralta and J. Segura, J. Chromatogr. Sei., 1974, 12, 701.Google Scholar
  15. 15).
    J. Segura, F. Artigas, E. Martínez and E. Gelpi, Biomed. Mass Spectrom., 1976, 3 91•Google Scholar
  16. 16).
    A.A. Boulton and G.B. Baker, J. Neurochem., 1975, 25, 477.CrossRefGoogle Scholar
  17. 17).
    L. Bertilsson and L. Palmer, Science, 1972, 177, 74.Google Scholar
  18. 18).
    S.J. Dencker, V. Malm, B.E. Roos and B. Werdinius, J. Neurochem., 1966, 13, 1545.Google Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • F. Artigas
    • 1
  • E. Gelpí
    • 1
  1. 1.Instituto de Biofísica y NeurobiologíaC.S.I.C.BarcelonaSpain

Personalised recommendations