Vitamin D pp 497-521 | Cite as

Measurement of Vitamin D and its Metabolites by Gas Chromatography-Mass Spectrometry

  • Hugh L. J. Makin
  • David J. H. Trafford

Abstract

Gas-liquid chromatography (GC) has been very successfully applied to the analysis of a number of different groups of biologically important compounds. In particular, the analysis of the multiplicity of metabolites of secreted steroid hormones found in human urine has been greatly simplified by the introduction of GC (1). Using conventional packed columns (2) and wall-coated capillary columns (3), ‘profiles’ of varying degrees of complexity have been produced. Plasma steroids have also been analysed by GC using electron capture detection (4) and more recently using gas chromatography-mass spectrometry (GC-MS, 5).

Keywords

Cholesterol HPLC Anhydride Aldosterone Antimony 

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Copyright information

© Martinus Nijhoff Publishing, Boston/The Hague/Dordrecht/Lancaster 1984

Authors and Affiliations

  • Hugh L. J. Makin
  • David J. H. Trafford

There are no affiliations available

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