Protein Binding Estimates in the Presence of Volume Shifts During Equilibrium Dialysis
With the advent of translucent acrylic plastic cells, it has become apparent that sizable and variable volume shifts occur across a semipermeable membrane during equilibrium dialysis. These volume shifts, toward the plasma or protein side, influence the estimation of binding parameters and the unbound fraction. A method of correcting for volume shifts is presented using binding data for salicylate and prednisolone. The specific dialysis procedures employed have been described elsewhere (1,2).
KeywordsRenal Transplant Patient Binding Data Unbind Fraction Equilibrium Dialysis Semipermeable Membrane
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