Protein Binding Estimates in the Presence of Volume Shifts During Equilibrium Dialysis

  • Thomas N. Tozer


With the advent of translucent acrylic plastic cells, it has become apparent that sizable and variable volume shifts occur across a semipermeable membrane during equilibrium dialysis. These volume shifts, toward the plasma or protein side, influence the estimation of binding parameters and the unbound fraction. A method of correcting for volume shifts is presented using binding data for salicylate and prednisolone. The specific dialysis procedures employed have been described elsewhere (1,2).


Renal Transplant Patient Binding Data Unbind Fraction Equilibrium Dialysis Semipermeable Membrane 
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  1. 1.
    D.E. Furst, T.N. Tozer and K.L. Melmon, Salicylate clearance, the resultant of protein binding and metabolism, Clin. Pharmacol. Ther. 26: 380 – 389 (1979).PubMedGoogle Scholar
  2. 2.
    J.G. Gambertoglio, F.J. Frey, N.H.G. Holford, J.L. Birnbaum, P.S. Lisak, F. Vincenti, N.J. Feduska, O. Salvatierra and W.J.C. Amend, Prednisone and prednisolone bioavailability in renal transplant patients, Kidney Int. 21: 621 – 626 (1982).PubMedCrossRefGoogle Scholar
  3. 3.
    T.N. Tozer, J.G. Gambertoglio, D.E. Furst, D.S. Avery and N.H.G. Holford, Volume shifts and protein binding estimates using equilibrium dialysis - application to prednisolone binding in man, J. Pharm. Sci. 72: 1442 – 1446 (1983).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Thomas N. Tozer
    • 1
  1. 1.Department of PharmacyUniversity of CaliforniaSan FranciscoUSA

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