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Cathepsin B-Like Cysteine Proteinases and Metastasis

  • Bonnie F. Sloane
  • Jurij Rozhin
  • Randall E. Ryan
  • Tamara T. Lah
  • Nancy A. Day
  • John D. Crissman
  • Kenneth V. Honn
Part of the Developments in Oncology book series (DION, volume 40)

Abstract

During the process of metastasis tumor cells traverse several extracellular matrix barriers in order to gain entry to the vascular space at the site of the primary tumor and to the perivascular space at the sites of metastatic tumors. A number of in vitro model systems have been designed to study tumor cell invasion through extracellular matrices. Several of these model systems including systems to study the concomitant digestion of these matrices by tumor cells are discussed elsewhere in this volume (see Chapters 18–21). A focus of much recent research is the basement membrane which underlies endothelial cells. The physical location of the basement membrane suggests that it must be traversed for tumor cells to form hematogenous metastases. Since the basement membrane in contrast to other extracellular matrix barriers contains type IV collagen a number of investigators have hypothesized that tumor cells must be able to degrade type IV collagen in order to invade through the basement membrane (see also Chapter 19). Metastatic variants of the B16 melanoma have been shown to secrete a type IV collagenase (1). However, type IV collagenase is secreted by tumor cells in a latent form which requires activation (2) suggesting that type IV collagenase is not by itself sufficient for basement membrane invasion by tumor cells and that additional proteinases or a proteolytic cascade may participate in basement membrane invasion.

Keywords

Cysteine Proteinase Cysteine Proteinase Inhibitor Plasma Membrane Fraction Lipoxygenase Product Centrifugal Elutriation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishing, Boston 1986

Authors and Affiliations

  • Bonnie F. Sloane
  • Jurij Rozhin
  • Randall E. Ryan
  • Tamara T. Lah
  • Nancy A. Day
  • John D. Crissman
  • Kenneth V. Honn

There are no affiliations available

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