Abstract
Plasma fractionation on a large scale (batch sizes 1000–5000 litres) is still carried out on the basis of the methods 6 and 9 developed by E.J. Cohn. However, cold ethanol precipitation is generally restricted to the production of albumin and intra-muscular IgG. Of all the other plasma proteins produced for therapeutic use, Factor VIII is probably the only protein not produced on a routine basis by chromatography. Thus factor IX concentrate, antithrombin III, fibronectin, C-l esterase inhibitor are produced chromatographically as side fractions of ethanol precipitation (1). For these proteins and for specific immune globulins, especially anti-D(Rh) IgG, ion exchange or affinity chromatography are the current methods of choice.
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© 1985 Martinus Nijhoff Publishing, Boston
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Curling, J.M. (1985). Chromatographic Methods of Plasma Fractionation. In: Smit Sibinga, C.T., Das, P.C., Seidl, S. (eds) Plasma Fractionation and Blood Transfusion. Developments in Hematology and Immunology, vol 13. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2631-1_10
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DOI: https://doi.org/10.1007/978-1-4613-2631-1_10
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