Abstract
Relationships between in vitro growth factor autonomy, replicative capacity, differentiation, and leukemogenicity were investigated using murine myelogenous leukemia cells derived from Abelson virus (A-MuLV) leukemic mice and interleukin-3 dependent hemopoietic cells infected in vitro with A-MuLV. All promonocytic cell lines cloned from A-MuLV leukemic mice were growth factor independent in vitro and tumorigeneic in vivo Separate, cloned lines from individual leukemic mice differed in their responsiveness to granulocyte-macrophage colony stimulating activity (GM-CSA), and in their capacity to differentiate. Differentiation of cloned lines in vitro led to a reduction of in vitro replicative capacity and tumorigenicity. However, clonal lines which did not differentiate in vitro in response to GM-CSA, were isolated from all leukemias. Growth factor dependent, non-leukemogenic hemopoietic progenitor cells (FDPC), derived from long term bone marrow cultures, were grown in interleukin-3 containing conditioned medium and infected with A-MuLV. Growth-factor independent colony forming cells could be identified as early as one week after infection. The growth factor autonomous cells produced A-MuLV, GM-CSA, a beta-type transforming growth factor activity, and were able to support the growth of factor dependent FDPC.
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© 1985 Martinus Nijhoff Publishing, Boston
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Hines, D.L. (1985). Clonal Variation and Phenotypic Progression in Retrovirus Transformed Leukemia Cells. In: Baker, L., Valeriote, F., Ratanatharathorn, V. (eds) Biology and Therapy of Acute Leukemia. Developments in Oncology, vol 33. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2609-0_6
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DOI: https://doi.org/10.1007/978-1-4613-2609-0_6
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