Organization and Control of the mRNA of the HSV TK Gene

  • James R. Smiley
Part of the Developments in Molecular Virology book series (DMVI, volume 7)


The sequences necessary for the activation of HSV TK gene transcription by HSV immediate-early proteins are largely coincident with the upstream region of the TK promoter. The implications of this arrangement are discussed.


Herpes Simplex Virus Type Thymidine Kinase Xenopus Oocyte Uninfected Cell Promoter Site 
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  1. 1.
    Dubbs D, Kit S: Mutant strains of herpes simplex deficient in thymidine kinase-inducing activity. Virology (22): 493–502, 1964.PubMedCrossRefGoogle Scholar
  2. 2.
    Munyon W, Kraiselburd E, Davis D, Mann J: Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus. J Virol (23): 234, 1971.Google Scholar
  3. 3.
    Wigler M, Silverstein S, Lee L, Pellicer A, Cheng Y, Axel R: Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell (11): 223–232, 1977.PubMedCrossRefGoogle Scholar
  4. 4.
    Colbere-Garapin F, Chousterman S, Horodniceau F, Kourilsky P, Garapin A: Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in E. coli K12. Proc Natl Acad Sci USA (76): 3755–3759, 1979.PubMedCrossRefGoogle Scholar
  5. 5.
    McKnight S, Gavis E: Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro. Nucl Acids Res (8): 5931–5948, 1980.PubMedCrossRefGoogle Scholar
  6. 6.
    Wilkie N, Clements J, Boll W, Mantei N, Lonsdale D, Weissman C: Hybrid plasmids containing an active thymidine kinase gene of herpes simplex virus — 1. Nucl Acids Res (7): 859–877, 1979.PubMedCrossRefGoogle Scholar
  7. 7.
    McKnight S: The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. Nucl Acids Res (8): 5949–5964, 1980.PubMedCrossRefGoogle Scholar
  8. 8.
    Wagner M, Sharp J, Summers W: Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1. Proc Natl Acad Sci USA (78): 1441–1445, 1981.PubMedCrossRefGoogle Scholar
  9. 9.
    Smiley J, Wagner M, Summers W, Summers W: Genetic and physical evidence for the polarity of transcription of the thymidine kinase gene of herpes simplex virus. Virology (102): 83–93, 1980.PubMedCrossRefGoogle Scholar
  10. 10.
    Proudfoot N, Brownlee G: Sequence at the 3′ end of globin mRNA shows homology with immunoglobin light chain mRNA. Nature (252): 359–362, 1974.PubMedCrossRefGoogle Scholar
  11. 11.
    Fitzgerald M, Shenk T: The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs. Cell (24): 251–260, 1981.PubMedCrossRefGoogle Scholar
  12. 12.
    Preston C, McGeoch D: Identification and mapping of two polypeptides encoded within the herpes simplex virus type 1 thymidine kinase gene sequences. J Virol (38): 593–605, 1981.PubMedGoogle Scholar
  13. 13.
    Marsden H, Haar L, Preston C: Processing of herpes simplex virus proteins and evidence that translation of thymidine kinase mRNA is initiated at three separate AUG codons. J Virol (46): 434–445, 1983.PubMedGoogle Scholar
  14. 14.
    Constanzo F, Campadeli-Fiume G, Foa-Tomasi L, Cassai E: Evidence that herpes simplex virus DNA is transcribed by cellular RNA polymerase B. J Virol (21): 996–1001, 1977.Google Scholar
  15. 15.
    Honess R, Roizman B: Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. J Virol (14): 8–19, 1974.PubMedGoogle Scholar
  16. 16.
    Watson R, Preston C, Clements J: Separation and characterization of herpes simplex virus type 1 immediate-early mRNA’s. J Virol (31): 42–52, 1979.PubMedGoogle Scholar
  17. 17.
    Hackern S, Roizman B: Regulation of herpesvirus macromolecular synthesis: transcription-initiation sites and domains of a genes. Proc Natl Acad Sci USA (77):7122–7126, 1980.CrossRefGoogle Scholar
  18. 18.
    Anderson K, Costa R, Holland L, Wagner E: Characterization of herpes simplex virus type 1 RNA present in the absence of de novo protein synthesis. J Virol (34): 9–27, 1980.PubMedGoogle Scholar
  19. 19.
    Watson R, Clements J: Characterization of transcription-deficient temperature-sensitive mutants of herpes simplex virus type 1. Virology (91): 364–369, 1978.PubMedCrossRefGoogle Scholar
  20. 20.
    Preston C: Control of herpes simplex virus type 1 mRNA synthesis in cells infected with wild-type virus or the temperature-sensitive mutant tsK. J Virol (29): 275–284, 1979.PubMedGoogle Scholar
  21. 21.
    Watson R, Clements J: A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesis. Nature (285): 329–330, 1980.PubMedCrossRefGoogle Scholar
  22. 22.
    Post L, Hackern S, Roizman B: Regulation of α genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with α gene promoters. Cell (24): 555–565, 1981.PubMedCrossRefGoogle Scholar
  23. 23.
    Mackem S, Roizman B: Differentiation between α promoter and regulator regions of herpes simplex virus 1: the functional domains and sequence of a moveable α regulator. Proc Natl Acad Sci USA (79): 4917–4921, 1982.PubMedCrossRefGoogle Scholar
  24. 24.
    Batterson W, Roizman B: Characterization of the herpes simplex virion-associated factor responsible for the induction of α genes. J Virol (46): 371–377, 1983.PubMedGoogle Scholar
  25. 25.
    Mackem S, Roizman B: Regulation of genes of herpes simplex virus: the α 27 gene promoter-thymidine kinase chimera is positively regulated in coverted L cells. J Virol (43): 1015–1023, 1982.PubMedGoogle Scholar
  26. 26.
    Mackem S, Roizman B: Structural features of the herpes simplex virus a gene 4, 0, and 27 promoter-regulatory sequences which confer α regulation on chimeric thymidine kinase genes. J Virol (44): 939–949, 1982.PubMedGoogle Scholar
  27. 27.
    Frink R, Draper K, Wagner E: Uninfected cell polymerase efficiently transcribes early but not late herpes simplex virus type 1 mRNA. Proc Natl Acad Sci USA (78): 6139–6143, 1981.PubMedCrossRefGoogle Scholar
  28. 28.
    Holland L, Anderson K, Shipman C, Wagner E: Viral DNA synthesis is required for the efficient expression of specific herpes simplex virus type 1 mRNA species. Virology (101): 10–24, 1980.PubMedCrossRefGoogle Scholar
  29. 29.
    Frink R, Eisenberg R, Cohen G, Wagner E: Detailed analysis of the portion of the herpes simplex virus type 1 genome encoding glycoprotein C. J Virol (45): 634–647, 1982.Google Scholar
  30. 30.
    Garfinkle B, McAuslan B: Regulation of herpes simplex virus-induced thymidine kinase. Biochem Biophys Res Comm (58): 822–829, 1974.PubMedCrossRefGoogle Scholar
  31. 31.
    Leung W: Evidence for a herpes simplex virus-specific factor controlling the transcription of deoxypyrimidine kinase. J Virol (27): 269–274, 1978.PubMedGoogle Scholar
  32. 32.
    Leung W, Dimock K, Smiley J, Bacchetti S: Herpes simplex virus thymidine kinase transcripts are absent from both nucleus and cytoplasm during infection in the presence of cycloheximide. J Virol (36): 361–365, 1980.PubMedGoogle Scholar
  33. 33.
    Sharp J, Wagner H, Summers W: Transcription of herpes simplex virus genes in vivo: Overlap of a late promoter with the 3′ end of the early thymidine kinase gene. J Virol (45): 10–17, 1983.PubMedGoogle Scholar
  34. 34.
    Bacchetti S, Graham F: Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA. Proc Natl Acad Sci USA (74): 1590–1594, 1977.PubMedCrossRefGoogle Scholar
  35. 35.
    Harland R, Weintraub H, McKnight S: Transcription of DNA injected into Xenopus oocytes is influenced by template topology. Nature (302): 38–43, 1983.PubMedCrossRefGoogle Scholar
  36. 36.
    Smiley J, Steege D, Juricek D, Summers W, Ruddle F: A herpes simplex virus 1 integration site in the mouse genome defined by somatic cell genetic analysis. Cell (15): 455–468, 1978.PubMedCrossRefGoogle Scholar
  37. 37.
    Pellicer A, Wigler M, Axel R, Silverstein S: The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell (14): 133–141, 1978.PubMedCrossRefGoogle Scholar
  38. 38.
    Luciw R, Bishop J, Varmus H, Capecchi M: Location and function of retroviral and SV40 sequences that enhance biochemical transformation after microinjection of DNA. Cell (33): 705–716, 1983.PubMedCrossRefGoogle Scholar
  39. 39.
    Corden J, Wasylyk B, Buchwalder A, Sassone-Corsi P, Kedinger D, Chambon P: Promoter sequences of eukaryotic protein-coding genes. Science (209): 1406–1413, 1980.PubMedCrossRefGoogle Scholar
  40. 40.
    Grosschedl R, Birnstiel M: Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutations in vivo. Proc Natl Acad Sci USA (77): 1432–1436, 1980.PubMedCrossRefGoogle Scholar
  41. 41.
    Benoist C, O’Hare K, Breathnach R, Chambon P: The ovalbumin gene-sequence of putative control regions. Nucl Acids Res (8): 127–142, 1960.CrossRefGoogle Scholar
  42. 42.
    McKnight S, Gavis E, Kingsbury R, Axel R: Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region. Cell (25): 385–398, 1981.PubMedCrossRefGoogle Scholar
  43. 43.
    McKnight S, Kingsbury R: Transcriptional control signals of a eukaryotic protein-coding gene. Science (217): 316–324, 1982.PubMedCrossRefGoogle Scholar
  44. 44.
    McKnight S: Functional relationships between transcriptional control signals of the thymidine kinase gene of herpes simplex virus. Cell (3): 355–365, 1982.CrossRefGoogle Scholar
  45. 45.
    Read G, Summers W: In vitro transcription of the thymidine kinase gene of herpes simplex virus. Proc Natl Acad Sci USA (79): 5215–5219, 1982.PubMedCrossRefGoogle Scholar
  46. 46.
    Roberts J, Axel R: Gene amplification and gene correction in somatic cells. Cell (29): 109–119, 1982.PubMedCrossRefGoogle Scholar
  47. 47.
    Ostrander M, Vogel S, Silverstein S: Phenotypic switching in cells transformed with the herpes simplex virus thymidine kinase gene. Mol Cell Biol (2): 708–714, 1982.PubMedGoogle Scholar
  48. 48.
    Zipser D, Lipsich L, Kwoh J: Mapping functional domains in the promoter region of the herpes thymidine kinase gene. Proc Natl Acad Sci USA (78): 6276–6280, 1981.PubMedCrossRefGoogle Scholar
  49. 49.
    Smiley J, Swan H, Pater M, Pater A, Halpern M: Positive control of the herpes simplex virus thymidine kinase gene requires upstream DNA sequences. J Virol (47): 301–310, 1983.PubMedGoogle Scholar
  50. 50.
    Lin S, Munyon W: Expression of the viral thymidine kinase gene in herpes simplex virus-transformed cells. J Virol (14): 1199–1208, 1974.PubMedGoogle Scholar
  51. 51.
    Leiden J, Buttyan R, Spear P: Herpes simplex virus gene expression in transformed cells. I. Regulation of the viral thymidine kinase gene in transformed L cells by products of superinfecting virus. J Virol (20): 413–424, 1976.PubMedGoogle Scholar
  52. 52.
    Kit S, Dubbs D: Regulation of herpesvirus thymidine kinase activ in LM (TK-) cells transformed by ultraviolet light-irradiated her simplex virus. Virology (76): 331–340, 1977.PubMedCrossRefGoogle Scholar
  53. 53.
    Freeman M, Powell K: DNA-binding properties of a herpes simplex virus immediate-early protein. J Virol (44): 1084–1087, 1982.PubMedGoogle Scholar
  54. 54.
    Feldman L, Imperiale M, Nevins J: Activation of early adenovirus transcription by the herpesvirus immediate early gene: Evidence for a common cellular control factor. Proc Natl Acad Sci USA (79): 4952–4956, 1982.PubMedCrossRefGoogle Scholar
  55. 55.
    Draper K, Frink R, Wagner E: Detailed characterization of an apparently unspliced herpes simplex virus type 1 gene mapping in the interior of another. J Virol (43): 1123–1128, 1982.PubMedGoogle Scholar
  56. 56.
    Nevins J: Mechanisms of activation of early viral transcription by the adenovirus E1A gene product. Cell (26): 213–230.Google Scholar

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© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • James R. Smiley

There are no affiliations available

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