The Interactions of Viral Proteins with Rous Sarcoma Virus RNA and Possible Control of Reverse Transcription, Translation and Virion Assembly

  • Jean-Luc Darlix
  • Claude Meric
  • Pierre-Francois Spahr
Part of the Developments in Molecular Virology book series (DMVI, volume 7)

Summary

The interactions between Rous Sarcoma virus RNA and the viral proteins have been analysed in the virus and in vitro. We show that it is protein P12 and not P19 that binds tightly to RSV RNA both in the virus and in vitro. Specific RNA sequences are recognized by protein P12 in the virus and such sequences are close to or involve the splice sites, and the dimer linkage site likely to be required for viral packaging. In vitro P12 does not impair proviral DNA synthesis whereas it can completely inhibit RNA translation. Such data suggest that P12 may control negatively viral RNA splicing and translation and positively the packaging process.

Keywords

Sucrose Glycerol Filtration Urea Citrate 

Abbreviations used

RSV

Rous sarcoma virus

PAGE

olyacrylamide gel electrophoresis

UV

ultraviolet light

nt

nucleotides

RNP

ribonucleoprotein

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Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • Jean-Luc Darlix
  • Claude Meric
  • Pierre-Francois Spahr

There are no affiliations available

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