Replication Scheme for Hepatitis B and Related Viruses
The preceding sections of this monograph indicate that hepatocyte membrane associated HBsAg, nuclear HBcAg and HBeAg, serum Dane particles, DNA Polymerase activity and serum derived HBeAg forms correlate with virus replication. Examination of these markers in HBV infected carriers suggests the existence of two HBV replication patterns. One pattern (group 1) has been characterized by high concentrations of serum HBsAg, HBeAg and high DNA Polymerase activity (640, 758, 759). These carriers also demonstrate high levels of genome length DNA in both serum and liver (653, 654). Both Dane particles and small spherical HBsAg from these individuals often have detectable receptor for polymerized albumin. The other pattern (group 2) has been characterized by lower concentrations of HBsAg and usually no HBeAg, DNA Polymerase activity, or detectable HBV associated receptor for polymerized albumin. Group 2 carriers demonstrate genome length DNA in the liver but not in the serum. Other carriers, demonstrating very low serum HBsAg concentrations and having only integrated DNA, probably do not replicate HBV (653, 654). Analysis of the viral DNA forms in each group showed that groups 1 and 2 contained the nick and gap form and that group 1 additionally contained supercoiled molecules in both the liver and serum (653, 654).
KeywordsSucrose Hepatitis Albumin Agarose Sedimentation
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