Abstract
Once a DNA fragment is cloned in a bacterial plasmid, it becomes an object for all the bacterial systems that control plasmid replication, maintenance, gene expression and recombination. While the first systems ensure faithful amplification of the cloned DNA, and if applicable, its expression in the host bacteria, bacterial recombination systems may lead to duplications, deletions and rearrangements of cloned DNA fragments. Conservation of the nucleotide sequence during cloning procedure and during propagation of the chimera plasmids is an absolute requirement for any investigation of the structure and function of the cloned fragment at its place of origin. Measures must therefore be taken to minimize deletions and rearrangements of cloned DNA in the host bacteria. The nature of the elements which affect the integrity of cloned DNA is the scope of this manuscript.
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© 1985 Martinus Nijhoff Publishing, Boston
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Cohen, A. (1985). Cloned DNA as a Substrate of Bacterial Recombination System. In: Becker, Y., Hadar, J. (eds) Recombinant DNA Research and Viruses. Developments in Molecular Virology, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2565-9_4
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DOI: https://doi.org/10.1007/978-1-4613-2565-9_4
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