Cloning of Human Oncogenes

  • Lee Ratner
  • Robert C. Gallo
  • Flossie Wong-Staal
Part of the Developments in Molecular Virology book series (DMVI, volume 5)


Human oncogenes homologous to v-fes, v-myb, v-myc, and v-sis have been cloned in recombinant phage vectors. Their structures have been elucidated by restriction enzyme and heteroduplex analyses, and in the case of c-sis by nucleotide sequencing as well. The complexity of these human genomic sequences is greater than that of the retroviral oncogenes, including multiple intervening sequences. In the cases of c-myb, c-myc, and c-sis, additional exons have been found by hybridization to mRNA or cDNA samples, compared to those characterized by hybridization to the retroviral oncogenes. In addition, a cDNA-containing plasmid with sequences of human c-sis mRNA has been isolated which can transform 3T3 fibroblasts. By somatic cell hybrid analysis and in situ hybridization, the chromosomal location of each of these oncogene homologues was determined, and compared to the chromosomal breakpoints in translocations in specific malignancies.


Long Terminal Repeat Chronic Myelogenous Leukemia Chronic Myelogenous Leukemia Patient Avian Leukosis Virus Woolly Monkey 
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  1. 1.
    Maniatis, T., Madison, P.C., Lacy, E., Lauer, J., O’Connell, C., Quon, D., Sin, G.K. and Esfratiadis, A. Cell 15:687–701, 1978.PubMedCrossRefGoogle Scholar
  2. 2.
    Maniatis, T., Fritsch, E.F. and Sambrook, J. Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, 1982.Google Scholar
  3. 3.
    Okayama, H. and Berg, P. Mol. Cell. Biol. 2:161–170, 1982.PubMedGoogle Scholar
  4. 4.
    Gardner, M.B., Rongey, R.W., Anstein, P. Estes, J.D., Sarma, P., Huebner, R.J. and Richard, G. G. Nature 226:807–809, 1970.PubMedCrossRefGoogle Scholar
  5. 5.
    McDonough, S.K., Larsen, S., Brodey, R.S., Stock, N.D. and Hardy, W.D., Jr. Cancer Res. 31:953–956, 1971.PubMedGoogle Scholar
  6. 6.
    Snyder, S.P. and Theilen, G.H. Nature 221:1074–1075, 1969.PubMedCrossRefGoogle Scholar
  7. 7.
    Frankel, A.C., Gilbert, J.H., Porzig, K.J., Scolnick, E.M. and Aaronson, S.A. J. Virol. 20:821–827, 1979.Google Scholar
  8. 8.
    Shibuya, M., Hanafusa, T., Hanafusa, H. and Stephenson, J.R. Proc. Natl. Acad. Sci. USA 77:6536–6540, 1980.PubMedCrossRefGoogle Scholar
  9. 9.
    Franchini, G., Gelmann, E.P., Dalla Favera, R., Gallo, R.C. and Wong-Staal, F. Mol. Cell. Biol. 2:1014–1019, 1982.PubMedGoogle Scholar
  10. 10.
    Franchini, G., Even, J., Scherr, C.J. and Wong-Staal, F. Nature 290:154–157, 1981.PubMedCrossRefGoogle Scholar
  11. 11.
    Scherr, C.J., Fedele, L.A., Oskarsson, M., Maizel, J., Vande Woude, G.F. J. Virol. 34: 200–202, 1980.Google Scholar
  12. 12.
    Van Beveren, C., Galleshaw, J.A., Jonas, V., Berns, A.J.M., Doolittle, R., Donoghue, D.J. and Verma, I.M. Nature 289:258–262, 1981.PubMedCrossRefGoogle Scholar
  13. 13.
    Goff, S.P., Gilboa, E., Witte, O.N. and Baltimore, D. Cell 22:777–785, 1980.PubMedCrossRefGoogle Scholar
  14. 14.
    Takeya, T. and Hanafusa, H. Cell 22:881–890, 1983.CrossRefGoogle Scholar
  15. 15.
    Chen, I.S.Y., Wilhelmsen, K.C. and Temin, H.M. J. Virol. 45:104–113, 1983.PubMedGoogle Scholar
  16. 16.
    Dhar, I.R., Ellis, R.W., Oroszlan, S., Shapiro, B., Maizel, J., Lowy, D. and Scolnick, E. Science 217:934–936, 1982.PubMedCrossRefGoogle Scholar
  17. 17.
    Capon, D.J., Chen, E.Y., Levinson, A.D., Seeburg, D.H. and Goeddel, D.V. Nature 302:33–37, 1983.PubMedCrossRefGoogle Scholar
  18. 18.
    Ellis, A.W., Lowy, D.R. and Scolnick, E.M. In: Advances in Viral Oncology (Ed. G. Klein), Raven Press, New York, 1982, Vol. 1, pp. 107–126.Google Scholar
  19. 19.
    Dalla Favera, R., Franchini, G., Martinotti, S., Wong-Staal, F., Gallo, R.C. and Croce, C.M. Proc. Natl. Acad. Sci. USA 79:4714–4717, 1980.Google Scholar
  20. 20.
    Heisterkamp, N., Groffen, J., Stephenson, J.R., Spurr, N.K., Goodfellow, P.N., Solomon, E., Carritt, B. and Bodmer, W.F. Nature 299:747–750, 1982.PubMedCrossRefGoogle Scholar
  21. 21.
    Harper, M.E., Franchini, G., Love, J., Simon, M.I., Gallo, R.C. and Wong-Staal, F. Nature 304:169–171, 1983.PubMedCrossRefGoogle Scholar
  22. 22.
    Rowley, J.D., Golomb, H.M. and Doughterty, C. Lancet i: 549–550, 1977.CrossRefGoogle Scholar
  23. 23.
    Graf, T. and Beug, H. Biochim. Biophys. Acta. 516:269–299, 1978.PubMedGoogle Scholar
  24. 24.
    Rushlow, K.M., Lautenberger, J.A., Papas, T.S., Baluda, M.A., Perbal, B., Chirikjian, J.G. and Reddy, E.P. Science 216:1421–1423, 1982.PubMedCrossRefGoogle Scholar
  25. 25.
    Franchini, G., Wong-Staal, F., Baluda, M.A., Lengel, C. and Tronick, S.R. Proc. Natl. Acad. Sci. USA 80:7385–7389, 1983.PubMedCrossRefGoogle Scholar
  26. 26.
    3rd International Workshop on Chromosomes in Leukemia. Cancer Res. 42:868–873, 1983.Google Scholar
  27. 27.
    Westin, E.H., Gallo, R.C., Arya, S.K., Eva, A., Souza, L.M., Baluda, M.A., Aaronson, S.A. and Wong-Staal, F. Proc. Natl. Acad. Sci. USA 29:2194–2198, 1982.Google Scholar
  28. 28.
    Wake, N., Hreshchyshyn, M.M., Piver, S.M., Matsui, S. and Sandberg, A.A. Cancer Res. 40:4512–4518, 1980.PubMedGoogle Scholar
  29. 29.
    Trent, J.M. and Salmon, S.E. Cancer Genet. Cytogenet. 2:279–291, 1981.CrossRefGoogle Scholar
  30. 30.
    Mitelman, F. and Levan, G. Hereditas 95:79–139, 1981.PubMedCrossRefGoogle Scholar
  31. 31.
    Hann, S.R., Abrams, H.D., Rohrscheider, L.R. and Eisenman, R.N. Cell 21:789–798, 1983.CrossRefGoogle Scholar
  32. 32.
    Dalla Favera, R., Gelmann, E.P., Martinotti, S., Franchini, G., Papas, T.S., Gallo, R.C. and Wong-Staal, F. Proc. Natl. Acad. Sci. USA 79:6497–6501, 1982.PubMedCrossRefGoogle Scholar
  33. 33.
    Watt, R., Stanton, L.W., Marcu, K.B., Gallo, R.C., Croce, C.M. and Rovera, G. Nature 303:725–728, 1983.PubMedCrossRefGoogle Scholar
  34. 34.
    Saito, H., Hayday, A.C., Wiman, K., Hayward, W.S. andTonegawa, S. Proc. Natl. Acad. Sci. USA 80:7476–7480, 1980.CrossRefGoogle Scholar
  35. 35.
    Stanton, L.W., Watt, R. and Marcu, K. Nature 303:401–406, 1983.PubMedCrossRefGoogle Scholar
  36. 36.
    Kohl, N.E., Kanda, N., Schreck, R.R., Bruns, G. Latt, S.A., Gilbert, F. and Alt, F.W. Cell 35:359–367, 1983.PubMedCrossRefGoogle Scholar
  37. 37.
    Dalla Favera, R., Brogni, M., Erikson, J., Patterson, D., Gallo, R.C. and Croce, CM. Proc. Natl. Acad. Sci. USA 79:7824–7827, 1982.CrossRefGoogle Scholar
  38. 38.
    Rabbitts,T.H., Forster, A., Baer, R. and Hamlyn, P.H. Nature 306:806–809, 1983.PubMedCrossRefGoogle Scholar
  39. 39.
    Croce, C.M., Thierfelder, W., Erikson, J., Nishikura, K., Finan, J., Lenoir, G. and Nowell, C. Proc. Natl. Acad. Sci. USA 80: 6922–6926, 1983.PubMedCrossRefGoogle Scholar
  40. 40.
    Erikson, J., Nishikura, K., Ar-Rushdi, A., Finan, J., Emanuel, B., Lenoir, G., Nowell, P.C. and Croce, C.M. Proc. Natl. Acad. Sci. USA 80:7581–7585, 1983.PubMedCrossRefGoogle Scholar
  41. 41.
    Hollis, C.F., Mitchell, K.F., Battey, J., Potter, H., Taub, R., Lenoir, C.M. and Leder, P. Nature 307:752–755, 1984.PubMedCrossRefGoogle Scholar
  42. 42.
    Gelmann, E.P., Psallidopoulos, M.C., Papas, T.S. and Dalla Favera, R. Nature 306:799–803, 1983.PubMedCrossRefGoogle Scholar
  43. 43.
    Rabbitts, T.H., Hamlyn, P.H. and Baer, R. Nature 306: 760–768, 1983.PubMedCrossRefGoogle Scholar
  44. 44.
    Battey, J., Moulding, C., Taub, R., Murphy, W., Stewart, T., Potter, H., Lenoir, G. and Leder, P. Cell 34:799–789, 1983.CrossRefGoogle Scholar
  45. 45.
    Hamlyn, P.H. and Rabbitts, T.H. Nature 304:135–139, 1983.PubMedCrossRefGoogle Scholar
  46. 46.
    Erikson, J., Ar-Rushi, A., Drwinga, H.L., Nowell, P.C. and Croce, C.M. Proc. Natl. Acad. Sci. USA 80:820–824, 1983.CrossRefGoogle Scholar
  47. 47.
    Klein, G. Nature 294:313–318, 1981.PubMedCrossRefGoogle Scholar
  48. 48.
    Nishikura, K., Ar-Rushdi, A., Erikson, J., Watt, R., Rovera, G. and Croce, C.M. Proc. Natl. Acad. Sci. USA 80:4822–4826, 1983.PubMedCrossRefGoogle Scholar
  49. 49.
    Shen-Ong, G.L.C., Keath, E.J., Piccoli, S.P. and Cole, M.D. Cell 31:443–452, 1982.PubMedCrossRefGoogle Scholar
  50. 50.
    Adams, J.M., Gerondakis, S., Webb, E., Mitchell, J., Bernard, O. and Cory, S. Proc. Natl. Acad. Sci. USA 7:6966–6970, 1982.Google Scholar
  51. 51.
    Wiener, F., Onno, S., Babonitz, M., Sumegi, J., Wirschubsky, Z., Klein, G., Mushinski, J.F. and Potter, M. Proc. Natl. Acad. Sci. USA 81:1159–1163, 1984.CrossRefGoogle Scholar
  52. 52.
    Calame, K., Kim, S., Lalley, P., Hill, R., Davis, S. and Hood, L. Proc. Natl. Acad. Sci. USA 79:6994–6998, 1982.PubMedCrossRefGoogle Scholar
  53. 53.
    Taub, R., Hirsch, I., Morton, C., Lenoir, G., Swan, D., Tronick, S., Aaronson, S. and Leder, P. Proc. Natl. Acad. Sci. USA 79:7837–7841, 1982.CrossRefGoogle Scholar
  54. 54.
    Theilen, G.H., Gould, D., Fowler, M. and Dungworth, D.L. J. Natl. Cancer Inst. 47:88l-889, 1971.Google Scholar
  55. 55.
    Gelmann, E.P., Wong-Staal, F., Kramer, R.A. and Gallo, R.C. Proc. Natl. Acad. Sci. USA 78:3373–3377, 1981.PubMedCrossRefGoogle Scholar
  56. 56.
    Robbins, K.C., Devare, S.G. and Aaronson, S.A. Proc. Natl. Acad. Sci. USA 78: 2918–2922, 1981.PubMedCrossRefGoogle Scholar
  57. 57.
    Gelmann, E.P., Petri, B., Cetta, A. and Wong-Staal, F. J. Virol. 41:593–604, 1982.PubMedGoogle Scholar
  58. 58.
    Wong-Staal, F., Dalla Favera, R., Gelmann, E.P., Manzari, V., Szala, S., Josephs, S.F. and Gallo, R.C. Nature 294:273–275, 1981.PubMedCrossRefGoogle Scholar
  59. 59.
    Devare, S.G., Reddy, E.P., Law, D.J., Robbins, K.C. and Aaronson, S.A. Proc. Natl. Acad. Sci. USA 80:731–735, 1983.PubMedCrossRefGoogle Scholar
  60. 60.
    Robbins, K.C., Antoniades, H.N., Devare, S.G., Hunkapiller M.W. and Aaronson, S.A. Nature 305:605–608, 1983.PubMedCrossRefGoogle Scholar
  61. 61.
    Doolittle, R.F., Hunkapiller, M.W., Hood, L.E., Devare, S.G., Robbins, K.C., Aaronson, S.A. and Antoniades, H. Science 221:275–277, 1983.PubMedCrossRefGoogle Scholar
  62. 62.
    Waterfield, M.D., Scrace, G.T., Stroobant, P., Wasteson, A., Westermark, B., Helden, C-H., Huang, J.S. and Deuel, T.F. Nature 304:35–39, 1983.PubMedCrossRefGoogle Scholar
  63. 63.
    Deuel, T.F., Huang, J.S., Proffitt, R.T., Chang, D. and Kennedy, B.B. J. Supramolec. Struct. Cell Biochem. Suppl. 5:128, 1981.Google Scholar
  64. 64.
    Deuel, T.F., Huang, J.S., Proffitt, R.T., Baenziger, J.U., Chang, D. and Kennedy, B.B. J. Biol. Chem. 256: 8896–8899, 1981.Google Scholar
  65. 65.
    Johnsson, A., Heldin, C-H., Westermark, B. and Wasteson, A. Biochem. Biophys. Res. Commun. 104:66–74, 1982.PubMedCrossRefGoogle Scholar
  66. 66.
    Antoniades, H.N. and Hunkapiller, M.W. Science 220:963–965, 1983.PubMedCrossRefGoogle Scholar
  67. 67.
    Dalla Favera, R., Gelmann, E.P., Gallo, R.C. and Wong-Staal, F. Nature 292:31–35, 1981.CrossRefGoogle Scholar
  68. 68.
    Josephs, S.F., Guo, C., Ratner, L. and Wong-Staal, F. Science 223:487–491, 1984.PubMedCrossRefGoogle Scholar
  69. 69.
    Josephs, S.F., Dalla Favera, R., Gelmann, E.P., Gallo, R.C. and Wong-Staal, F. Science 219:503–505, 1983.PubMedCrossRefGoogle Scholar
  70. 70.
    Dalla Favera, R., Gallo, R.C., Giallongo, A. and Croce, C.M. Science 218:686–688, 1982.CrossRefGoogle Scholar
  71. 71.
    Swan, D.C., McBride, O.W., Robbins, K.C., Keithley, D.A., Reddy, E.P. and Aaronson, S.A. Proc. Natl. Acad. Sci. USA 29:4691–4695, 1982.Google Scholar
  72. 72.
    Bartram, C.R., deKlein, A., Hagemeijer, A., Grosveld, G., Heisterkamp, N. and Groffen, J. Blood 63:223–225, 1984.PubMedGoogle Scholar
  73. 73.
    deKlein, A., Geurts van Kessel, A., Grosveld, G., Bartram, C.R., Hagemeijer, A., Bootsma, D., Spurr, N.K., Heisterkamp, N., Groffen, J. and Stephenson, J.R. Nature 300:765–767, 1982.CrossRefGoogle Scholar
  74. 74.
    Collins, S. and Groudine, M.T. Proc. Natl. Acad. Sci. USA 80:4813–4817, 1983.PubMedCrossRefGoogle Scholar
  75. 75.
    Selden, J.R., Emanuel, B., Wong, E., Cannizzaro, L., Polumbo, A., Erikson, J., Nowell, P.C., Rovera, G. and Croce, C.M. Proc. Natl. Acad. Sci. USA 80:7289–7292, 1983.PubMedCrossRefGoogle Scholar
  76. 76.
    Aurias, A., Rimbaut, C., Buffe, D., Dubosset, J. and Mazabraud, A. N. Engl. J. Med. 309:496–497, 1983.Google Scholar
  77. 77.
    Turc-Carel, C., Philip, I., Berger, M.P., Philip, T. and Lenoir, G.M. N. Engl. J. Med. 309:497–498, 1983.Google Scholar
  78. 78.
    Westin, E.H., Wong-Staal, F., Gelmann, E.P., Dalla Favera, R., Papas, T.S., Lautenberger, J.A., Eva, A., Reddy, E.P., Tronick, S.R., Aaronson, S.A. and Gallo, R.C. Proc. Natl. Acad. Sci. USA 79:2490–2494, 1982.PubMedCrossRefGoogle Scholar
  79. 79.
    Eva, A., Robbins, K.C., Andersen, P.R., Srinivasan, A., Tronick, S.R., Reddy, E.P., Ellmore, N.W., Galen, A.T., Lautenberger, J.A., Papas, T.S., Westin, E.H., Wong-Staal, F., Gallo, R.C. and Aaronson, S.A. Nature 295; 116–119, 1982.PubMedCrossRefGoogle Scholar
  80. 80.
    Dalla Favera, R., Wong-Staal, F. and Gallo, R.C. Nature 299:61–63, 1982.CrossRefGoogle Scholar
  81. 81.
    Nowell, P., Finan, J., Dalla Favera, R., Gallo, R.C., Ar-Rushdi, A., Ramaczuk, H., Rovera, G. and Croce, C.M. Nature 306:494–497, 1983.PubMedCrossRefGoogle Scholar
  82. 82.
    Little, C.D., Nau, M.M., Carney, D.N., Gazdar, A.F. and Mann, J.D. Nature 306:194–196, 1983.PubMedCrossRefGoogle Scholar
  83. 83.
    Alitalo, K., Schwab, M., Lin, C.C., Varmus, H.E. and Bishop, J.M. Proc. Natl. Acad. Sci. USA 80:1707–1711, 1983.PubMedCrossRefGoogle Scholar
  84. 84.
    Schwab, M., Alitalo, K., Varmus, H.E. and Bishop, J.M. Nature 303:497–501, 1983.PubMedCrossRefGoogle Scholar
  85. 85.
    Clarke, M.F., Westin, E., Schmidt, D., Josephs, S.F., Ratner, L., Wong-Staal, F., Gallo, R.C. and Reitz, J.S., Jr. Nature 308:464–467, 1984.PubMedCrossRefGoogle Scholar
  86. 86.
    Stiles, C.D. Cell 33:653–655, 1983.PubMedCrossRefGoogle Scholar
  87. 87.
    Kelley, K., Cochran, B.H., Stiles, C.D. and Leder, P. Cell 35:603–610, 1983.CrossRefGoogle Scholar
  88. 88.
    Land, H., Parada, L.F. and Weinberg, R.A. Nature 304: 596–602, 1983.PubMedCrossRefGoogle Scholar
  89. 89.
    Shimizu, K., Birnbaum, D., Ruley, M.A., Fasano, O., Suard, Y., Edlund, L., Taparowsky, E., Goldfarb, M. and Wigler, M. Nature 304:497–500, 1983.PubMedCrossRefGoogle Scholar
  90. 90.
    Nakano, H., Yamamoto, R., Neville, C., Evans, D., Mizuno, T. and Perucho, M. Proc. Natl. Acad. Sci. USA 81:71–75, 1984.PubMedCrossRefGoogle Scholar
  91. 91.
    Tabin, C.J., Bradley, S.M., Bargmann, C.I., Weinberg, R.A., Papageorge, A.G., Scolnick, E.M., Dhar, R., Lowy, D.R. and Chang, E.H. Nature 300:143–149, 1982.PubMedCrossRefGoogle Scholar
  92. 92.
    Reddy, E.P., Reynolds, R.K., Santos, E. and Barbacid, M. Nature 300:149–152, 1982.PubMedCrossRefGoogle Scholar
  93. 93.
    Taparowsky, E., Suard, Y., Fasano, O., Shimizu, K., Goldfarb, M. and Wigler, M. Nature 300:762–765, 1982.PubMedCrossRefGoogle Scholar
  94. 94.
    Capon, D.J., Seeburg, P.H., McGrath, J.P., Hayflick, J.S., Edman, U., Levinson, A.D. and Goeddel, D.V. Nature 304:507–513, 1983.PubMedCrossRefGoogle Scholar
  95. 95.
    Taparowsky, E., Shimizu, K., Goldfarb, M. and Wigler, M. Cell 21:581–586, 1983.CrossRefGoogle Scholar
  96. 96.
    Santos, E., Reddy, E.P., Pulciani, S., Feldmann, R.J. and Barbacid, M. Proc. Natl. Acad. Sci. USA 80:4679–4683, 1983.PubMedCrossRefGoogle Scholar
  97. 97.
    Santos, E., Martin-Zanca, D., Reddy, E.P., Pierotti, M.A., Delia Porta, G. and Barbacid, M. Science 223:661–664, 1984.PubMedCrossRefGoogle Scholar
  98. 98.
    Hayward, W.J., Neel, B.G. and Astrin, S.M. Nature 290: 475–480, 1981.PubMedCrossRefGoogle Scholar
  99. 99.
    Neel, B.G., Hayward, W., Robinson, M.L., Fang, J. and Astrin, S.M. Cell 23:323–334, 1981.PubMedCrossRefGoogle Scholar
  100. 100.
    Fung, Y.K., Faelly, A.M., Crittenden, L.B. and Kung, H.J. Proc. Natl. Acad. Sci. USA 78:3418–3422, 1981.CrossRefGoogle Scholar
  101. 101.
    Payne, G.S., Bishop, J.M. and Varmus, M.E. Nature 295:209–214, 1982.PubMedCrossRefGoogle Scholar
  102. 102.
    Fung, Y.K.T., Crittenden, L.B. and Kung, H.J. J. Virol. 44:742–746, 1982.PubMedGoogle Scholar
  103. 103.
    Noori-Dalooi, M.R., Swift, R.A., Kung, H.J., Witter, R.L. and Crittenden, L.B. Nature 294:574–576, 1981.CrossRefGoogle Scholar
  104. 104.
    Vennstrom, B. and Bishop, J.M. Cell 28:135–143, 1982.PubMedCrossRefGoogle Scholar
  105. 105.
    Fung, Y-K.T., Lewis, W.G., Crittenden, L.B. and Kung, H.J. Cell 33:357–368, 1983.PubMedCrossRefGoogle Scholar
  106. 106.
    Blair, D.G., McClements, W.L., Oskarsson, M., Fischinger, P. and Vande Woude, G.F. Proc. Natl. Acad. Sci. USA 77:3504–3508, 1980.PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • Lee Ratner
    • 1
  • Robert C. Gallo
    • 1
  • Flossie Wong-Staal
    • 1
  1. 1.Laboratory of Tumor Cell BiologyNational Cancer Institute, National Institutes of HealthBethesdaUSA

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