Cloning of Human Oncogenes

  • Lee Ratner
  • Robert C. Gallo
  • Flossie Wong-Staal
Part of the Developments in Molecular Virology book series (DMVI, volume 5)

Summary

Human oncogenes homologous to v-fes, v-myb, v-myc, and v-sis have been cloned in recombinant phage vectors. Their structures have been elucidated by restriction enzyme and heteroduplex analyses, and in the case of c-sis by nucleotide sequencing as well. The complexity of these human genomic sequences is greater than that of the retroviral oncogenes, including multiple intervening sequences. In the cases of c-myb, c-myc, and c-sis, additional exons have been found by hybridization to mRNA or cDNA samples, compared to those characterized by hybridization to the retroviral oncogenes. In addition, a cDNA-containing plasmid with sequences of human c-sis mRNA has been isolated which can transform 3T3 fibroblasts. By somatic cell hybrid analysis and in situ hybridization, the chromosomal location of each of these oncogene homologues was determined, and compared to the chromosomal breakpoints in translocations in specific malignancies.

Keywords

Carbohydrate Lymphoma Codon DMSO Leukemia 

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Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • Lee Ratner
    • 1
  • Robert C. Gallo
    • 1
  • Flossie Wong-Staal
    • 1
  1. 1.Laboratory of Tumor Cell BiologyNational Cancer Institute, National Institutes of HealthBethesdaUSA

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