Electrophysiological Studies of Cultured Mammalian CNS Neurons
Neurophysiology is an important area of neuroscience that is concerned in large part with two of the most important functions of neurons and neuronal networks, namely the membrane excitability of the individual elements and the process of intercellular communication of “information” into and throughout the CNS. Neurophysiology is often divided into two broad areas of investigation. One involves quantitative analysis of the basic membrane mechanisms underlying the generation of electrical and chemical signals. The other involves studies into how neuronal messages are communicated in the CNS. The complexity inherent in the vertebrate CNS has made it difficult to study the details underlying the excitable membrane properties and the various modes of intercellular signaling. The complexity of the vertebrate CNS has been reduced to monolayer simplicity through the innovative efforts of many investigators, including and especially Dr. Phillip Nelson and colleagues at the National Institutes of Health , who have developed methods for culturing neurons from the embryonic CNS.
KeywordsNeutral Amino Acid Spinal Neuron Spinal Cord Neuron Membrane Excitability Unitary Conductance
Unable to display preview. Download preview PDF.
- Barker J.L., McBurney R.N., and Mathers, D. A., 1983, Convulsant-induced depression of amino acid responses in cultured mouse spinal neurons studied under voltage clamp, B. J. Pharmacol. 80: 619–629.Google Scholar
- Barker, J., Dufy, B., Owen, D. G., and Segal, M., 1984, Excitable membrane properties of cultured central nervous system neurons and clonal pituitary cells, Cold Spring Harbor Symp. Quant. Biol. 48: 259–268.Google Scholar
- Ransom, B. R., Neale, E., Henkart, M., Bullock, P. N., and Nelson, P. G., 1977a, Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiological properties, J. Neurophysiol. 40: 1332–1150.Google Scholar
- Ransom, B. R., Christian, C. N., Bullock, P. N., and Nelson, P. G., 1977b, Mouse spinal cord in cell culture. II.Synaptic activity and circuit behavior, J. Neurophysiol. 40:1155–1162.Google Scholar
- Smith, T. G., Jr., Barker, J. L., Smith, B. M., and Colburn, T. R., 1981, Voltage clamp techniques applied to cultured skeletal muscle and spinal neurons, in: Excitable Cells in Tissue Culture( P. G. Nelson and M. Liberman, eds.), Plenum Press, New York, pp. 111–136.Google Scholar