Therapeutic Potential of Monoclonal Antibodies to the Leucocyte Common Antigen

  • Carol Bindon
  • Geoff Hale
  • Herman Waldmann


The leucocyte common (LC) antigen is a major glycoprotein family of leucocyte membranes. Monoclonal antibodies against the rat, mouse and human antigens have been described (Sunderland et al., 1979; Trowbridge, 1978; Dalchau et al., 1980). Its cell distribution and molecular weight are very similar in the three species. It is widely distributed on leucocytes, being found on T and B lymphocytes, thymocytes, macrophages and granulocytes, but is absent from other tissues. Its apparent molecular weight by SDS-PAGE is about 200 kilodaltons, but up to four species with slightly different mobilities have been observed according to the cell type studied (Dalchau and Fabre, 1981; Banga et al., 1984).


IgG2a Antibody Leukocyte Common Antigen Acute Myeloid Leukaemia Cell Rabbit Complement IgG2b Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aqel N.M., Clark M., Cobbold S.P. and Waldmann H., 1984, Immuno- histochemical screening in the selection of monoclonal antibodies. The use of isotype-specific antiglobulins, J. Immunol. Meth., in press.Google Scholar
  2. Banga J.P., Guarnotta G., Harte A., Pryce G., Campbell M.A., Quartey-Papafio R., Lydyard P.M., and Roitt I.M., 1984, A common epitope identified by a monoclonal antibody, MID2, present on all leukocytes and associated with a group of high molecular weight glycopeptides, Scand. J. Immunol., 19:11,Google Scholar
  3. Borsos T., and Rapp H.J., 1965, Complement fixation on cell surfaces by 19S and 7S antibodies, Science, 150: 505.PubMedCrossRefGoogle Scholar
  4. Boyse E.A., and 0ld,L.J., 1969, Some aspects of normal and abnormal cell surface genetics, Ann. Rev. Genetics, 3: 269.CrossRefGoogle Scholar
  5. Cobbold S.P., and Waldmann H., 1981, A rapid solid-phase enzyme- linked binding assay for screening monoclonal antibodies to cell surface antigens, J. Immunol. Meth., 44: 125.CrossRefGoogle Scholar
  6. Cobbold S.P., and Waldmann H., 1984, Therapeutic potential of monovalent monoclonal antibodies., Nature, 308: 460.PubMedCrossRefGoogle Scholar
  7. Dalchau R., Kirkley J., and Fabre J.W., 1980, Monoclonal antibody to a human leukocyte specific membrane glycoprotein probably homologous to the leukocyte common antigen of the rat, Eur. J. Immunol., 10: 737.PubMedCrossRefGoogle Scholar
  8. Dalchau R., and Fabre J.W., 1981, Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence for structural and possible functional diversity of the human leukocyte common molecule, J. Exp. Med., 153: 753.PubMedCrossRefGoogle Scholar
  9. Elliot E.V., Pinder A., Stevenson F.K., and Stevenson, G.T., 1978, Synergistic cytotoxic effects of antibodies directed against different cell surface determinants., Immunology, 34: 405.Google Scholar
  10. Fabre J.W., Sunderland C.A., and Williams A.F., 1980, The immunosuppressive properties of rabbit antibodies against a major glycoprotein restricted to rat leukocyte membranes, Transplantation, 23: 349.CrossRefGoogle Scholar
  11. Fust,G., Medgyesi,G.A., Bazin,H., and Gergely,J., 1980, Differences in the ability of rat IgG subclasses to consume complement in homologous and heterologous serum, Immunol. Lett., 1:249.Google Scholar
  12. Galfre G., Milstein C., and Wright B., 1979, Hat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG, Nature, 277: 131.PubMedCrossRefGoogle Scholar
  13. Glennie M.J., and Stevenson G.T., 1982, Univalent antibodies kill tumour cells in vitro and in vivo, Nature, 295: 712.PubMedCrossRefGoogle Scholar
  14. Hale G., Bright S., Chumbley G., Hoang T., Metcalf D., Munro A.J., Waldmann,H., 1983, Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement, Blood, 62: 873.PubMedGoogle Scholar
  15. Hansch G.M., Betz M., and Shin M., 1984, Cytolysis of nucleated cells by complement. Inhibition of membrane transmethylation enhances cell death by C5b-9, J. Immunol., 132: 1440.PubMedGoogle Scholar
  16. Hughes-Jones N.C., and Ghosh S., 1981, Anti-D-coated Rh positive red cells will bind the first component of the complement pathway, C1q, FEBS Letts., 128 (2): 318.CrossRefGoogle Scholar
  17. Hughes-Jones N.C., Gorick B.D., and Howard J.C., 1983, The mechanism of synergistic complement-mediated lysis of rat red cells by monoclonal IgG antibodies, Eur. J. Immunol., 13: 635.PubMedCrossRefGoogle Scholar
  18. Kessler S.W., 1975, Rapid isolation of antigens from cells with a staphlococcal protein A-antibody adsorbent: Parameters of the interaction of antibody-antigen complexes with protein A., J. Immunol., 115: 1617.PubMedGoogle Scholar
  19. Ledbetter J.A., and Seaman W.E., 1982, The Lyt-2, Lyt-3 macromolecules: structural and functional studies, Immunol. Rev., 68:197.PubMedCrossRefGoogle Scholar
  20. Metzger H., 1978, The effect of antigen on antibodies: Recent studies, Contemp. Top. Mol. Immunol., 7:119PubMedGoogle Scholar
  21. Springer T.A., Bhattacharya A., Cardoza J.T., and Sanchez-Madrid F., 1982, Monoclonal antibodies specific for rat IgG1, IgG2a and IgG2b subclasses and kappa chain monotypic and allotypic determinantss reagents for use with rat monoclonal antibodies, Hybridoma, 1:257.PubMedCrossRefGoogle Scholar
  22. Standring R., and Williams A.F., 1978, Glycoproteins and antigens of membranes prepared from rat thymocytes after lysis by shearing or with the detergent Tween-40, Biochim. Biophys. Acta, 508:85.CrossRefGoogle Scholar
  23. Sunderland C.A., McMaster W.R., and Williams A.F., 1979, Purification with monoclonal antbody of a predominant leukocyte common antigen and glycoprotein from rat thymocytes, Eur. J. Immunol., 9:155.Google Scholar
  24. Trowbridge I.S., 1978, Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200., J. Exp. Med., 148: 313.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Carol Bindon
    • 1
  • Geoff Hale
    • 1
  • Herman Waldmann
    • 1
  1. 1.Department of PathologyUniversity of CambridgeCambridgeUK

Personalised recommendations