Double Immunoenzymatic Staining Employing Rat and Mouse Monoclonal Antibodies
Double immunoenzymatic labelling has been described by Nakane, employing two different substrates for peroxidase staining1 and by Mason, employing alkaline phosphatase labelled antibodies2. In the procedure described by Nakane, no mixed staining of cells could be obtained when diaminobenzidin was used as a primary chromogen3,4. The method described by Mason, allows mixed staining of cells containing two different antigens.
KeywordsAcetone Lymphoma Germinal Myeloma Stein
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- 6.K. B. Pryzwanski, Applications of double label immune fluorescence, in: Techniques in Immunocytochemistry, G. R. Bullock and P. Petrusz, ed., Academic Press, London, (1982).Google Scholar