Double Immunoenzymatic Staining Employing Rat and Mouse Monoclonal Antibodies

  • S. Poppema
  • A. Boes
  • L. de Ley
  • T. Rozeboom
  • W. Timens
  • E. Schwander
  • L. Visser

Abstract

Double immunoenzymatic labelling has been described by Nakane, employing two different substrates for peroxidase staining1 and by Mason, employing alkaline phosphatase labelled antibodies2. In the procedure described by Nakane, no mixed staining of cells could be obtained when diaminobenzidin was used as a primary chromogen3,4. The method described by Mason, allows mixed staining of cells containing two different antigens.

Keywords

Acetone Lymphoma Germinal Myeloma Stein 

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References

  1. 1.
    P. K. Nakane, Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: A study on pituitary glands of the rat, J. Histochem. Cytochem. 16: 557 (1968).PubMedCrossRefGoogle Scholar
  2. 2.
    D. Y. Mason and R. Sammons, Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents, J. Clin. Pathol. 31: 454 (1978).PubMedCrossRefGoogle Scholar
  3. 3.
    S. Poppema, J. D. Elema and M. R. Halie, The significance of intracytoplasmic proteins in Reed-Sternberg cells, Cancer 42: 1793 (1978).PubMedCrossRefGoogle Scholar
  4. 4.
    L. A. Sternberger and S. A. Joseph, The unlabeled antibody method. Contrasting color staining of paired pituitary hormones without antibody removal, J. Histochem. Cytochem. 27: 1424 (1979).PubMedCrossRefGoogle Scholar
  5. 5.
    S. Poppema, A. K. Bhan, E. L. Reinherz, R. T. McCluskey and S. F. Schlossman, Distribution of T cell subsets in human lymph nodes, J. Exp. Med. 153: 30 (1981).PubMedCrossRefGoogle Scholar
  6. 6.
    K. B. Pryzwanski, Applications of double label immune fluorescence, in: Techniques in Immunocytochemistry, G. R. Bullock and P. Petrusz, ed., Academic Press, London, (1982).Google Scholar
  7. 7.
    R. C. Graham, U. Lundholm and M. J. Karnovsky, Cytochemical demonstration of peroxidase activity with 3-amino-9-ethyl- carbazole, J. Histochem. Cytochem. 13: 150 (1965).PubMedCrossRefGoogle Scholar
  8. 8.
    H. Stein, A. Bonk, G. Tolksdorf, K. Lennert, H. Rodt and J. Gerdes, Immunohistologic analysis of the organization of normal lymphoid tissue and non Hodgkin’s lymphomas, J. Histochem. Cytochem. 28: 746 (1980).PubMedCrossRefGoogle Scholar
  9. 9.
    N. L. Harris and R. E. Data, The distribution of neoplastic and normal B-lymphoid cells in nodular lymphomas, Hum. Pathol. 13: 610 (1982).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • S. Poppema
    • 1
  • A. Boes
    • 1
  • L. de Ley
    • 1
  • T. Rozeboom
    • 1
  • W. Timens
    • 1
  • E. Schwander
    • 1
  • L. Visser
    • 1
  1. 1.Departments of Pathology and ImmunologyUniversity of GroningenGroningenThe Netherlands

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