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Double Immunoenzymatic Staining Employing Rat and Mouse Monoclonal Antibodies

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Microenvironments in the Lymphoid System

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Abstract

Double immunoenzymatic labelling has been described by Nakane, employing two different substrates for peroxidase staining1 and by Mason, employing alkaline phosphatase labelled antibodies2. In the procedure described by Nakane, no mixed staining of cells could be obtained when diaminobenzidin was used as a primary chromogen3,4. The method described by Mason, allows mixed staining of cells containing two different antigens.

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References

  1. P. K. Nakane, Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: A study on pituitary glands of the rat, J. Histochem. Cytochem. 16: 557 (1968).

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Author information

Authors and Affiliations

  1. Departments of Pathology and Immunology, University of Groningen, Oostersingel 63, Groningen, 9713 EZ, The Netherlands

    S. Poppema, A. Boes, L. de Ley, T. Rozeboom, W. Timens, E. Schwander & L. Visser

Authors
  1. S. Poppema
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  2. A. Boes
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  3. L. de Ley
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  4. T. Rozeboom
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  5. W. Timens
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  6. E. Schwander
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  7. L. Visser
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Editor information

Editors and Affiliations

  1. Nationanl Institute for Medical Research, London, England

    G. G. B. Klaus

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© 1985 Plenum Press, New York

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Poppema, S. et al. (1985). Double Immunoenzymatic Staining Employing Rat and Mouse Monoclonal Antibodies. In: Klaus, G.G.B. (eds) Microenvironments in the Lymphoid System. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2463-8_93

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  • DOI: https://doi.org/10.1007/978-1-4613-2463-8_93

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9495-5

  • Online ISBN: 978-1-4613-2463-8

  • eBook Packages: Springer Book Archive

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