The Influence of Complement Receptors on the Localization of Lymphocytes in Vivo
T and B lymphocytes entering the spleen leave the blood stream in the sinuses of the marginal zone and will migrate from there into the white pulp of the spleen1. It has been demonstrated that there is a preference of B cells to localize in the spleen2–4 which is comparable to the localization patterns of lymphocytes in Peyer’s patches2. The splenic immigration of T-cell subsets, however, is more comparable with peripheral lymph nodes5. The selective entrance of T and B cells in the spleen is therefore different from that in the high endothelial venule (HEV)-bearing lymphoid organs such as lymph nodes and Peyer’s patches. How this selection in the spleen is accomplished is not known. In the marginal zone various macrophages can be identified6, 7 and it has been suggested that these cells play a role in lymphocyte traffic8–10. Macrophages can be influenced by complement factors11 and we therefore approached the question of selective lymphocyte migration in the spleen by looking at the effects of complement depletion by Cobra Venom (CVF) administration.
KeywordsCell Transfer High Endothelial Venule Normal Mouse Serum Cobra Venom Cobra Venom Factor
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