Abstract
Fluorescence microscopy (microfluorimetry) is an established technique that provides useful information about the biomolecules in a cellular environment[1,2]. The technique consists in exciting the samples (cells or part of them) with a light source of suitable wavelength, and in detecting and processing the fluorescence emission.
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References
S. Undenfriend, “Fluorescence Assay in Biology and Medicine,” Vol. II, Academic Press, New York (1969).
J. Duchesne, “Physicochemical Properties of Nucleic Acids,” Vol. I, Academic Press, New York (1973).
F. Ruch and U. Leeman, Cytofluorometry, in: “Micromethods in Molecular Biology,” V. Neuhoff, ed., Springer-Verlag, Berlin (1973).
G. Prenna, G. Bottiroli, and G. Mazzini, Histochem.J., 9: 15 (1977).
M. Kapoor, Biol.Rev., 47: 27 (1976).
S.A. Latt, Can.J.Genet.Cytol., 19: 603 (1977).
L. Brand and J. R. Gohlke, Ann.Rev.Biochem., 41: 843 (1972).
G. von Segenbusch and A. Thaer, “Fluorescence Techniques in Cell Biology,” A. Thaer and M. Sernetz, eds., Springer-Verlag, Berlin (1973).
F. W. D. Rost, “Fluorescence Techniques in Cell Biology,” A. Thaer and M. Sernetz, eds., Springer-Verlag, Berlin (1973).
S. Cova, G. Prenna, and G. Mazzini, Histochem.J., 6: 279 (1974).
A. Andreoni, A. Longoni, C. A. Sacchi, O. Svelto, and G. Bottiroli, “Tunable Lasers and Applications,” A. Mooradian, T. Jäger, and P. Stokseth, Springer-Verlag, Heidelberg, New York (1976).
G. Bottiroli, G. Prenna, A. Andreoni, C. A. Sacchi, and O. 13. Svelto, Photochem.Photobiol., 29: 23 (1979).
A. Andreoni, S. Cova, G. Bottiroli, and G. Prenna, Photochem. Photobiol., 29:951 (1979).
F. Docchio, R. Ramponi, C. A. Sacchi, G. Bottiroli, and I. Freitas, An automatic pulsed laser microfluorometer with high spatial and temporal resolution, J.Microscopy, in press.
R. Cubeddu, S. De Silvestri, and O. Svelto, Opt.Comm., 34: 460
F. Docchio, A. Longoni, and F. Zaraga, Rev.Sci.Instrum., 52: 1671
G. Bottiroli, P. G. Cionini, F. Docchio, and C. A. Sacchi, In situ evaluation of the functional state of chromatin by means of quinacrine mustard staining and time-resolved fluorescence microscopy, Histochem.J., 16: (1984) in press.
D. E. Comings, B. W. Kovacs, E. Avelino, and D. C. Harris, Chromosoma, 50: 111 (1975).
C. Nicolini, Basic Appi.Histochem., 25: 319 (1981).
T. J. Dougherty, J. E. Kaufman, A. Goldfarb, K. R. Weishaupt, D. Boyle, and A. Mittleman, Cancer Res., 38: 2628 (1978).
A. E. Profio, D. R. Doiron, and E. G. King, Med.Phys., 6: 523 (1979).
R. L. Lipson, E. J. Baldes, and A. M. Olsen, J.Natl.Cancer Inst., 26: 1 (1961).
J. Moan and S. Sommer, Photobiochem.Photobiophys., 3: 93 (1981).
R. Bonnet, R. J. Ridge, P. A. Scourides, and M. C. Rosenbaum, J.Chem.Soc.Perkin T., 12: 3135 (1981).
D. Kessel and T. Chow, Cancer Res., 43: 1994 (1983).
F. Docchio, R. Ramponi, C. A. Sacchi, G. Bottiroli, and I. Freitas, Time-resolved fluorescence spectroscopy of Hemato- porphyrin-Derivative (HpD) in human lymphocytes, Chem.Biol. Inter., in press.
G. Bottiroli, I. Freitas, F. Docchio, R. Ramponi, and C. A. Sacchi, The time-dependent behavior of Hematoporphyrin- Derivative in saline: A study of spectral modifications, Chem.Biol.Inter., in press.
J. Moan and S. Sommer, Photobiochem.Photobiophys., 3: 93 (1981).
S. B. Brown, H. Hatzikonstantinov, and D. G. Herries, Int.J.Biochem., 12:701 (1981).
W. J. M. Van der Putten and M. J. C. Van Gemert, Hematoporphy-rin-Derivative fluorescence spectra in vitro and an animal tumor, in: “Proc. Laser 81 Opto-Elektronik,” München, West Germany (1981).
G. Jori - personal communication.
M. W. Berns, A. Dahlman, F. M. Johnson, R. Burns, D. Sperling, M. Guiltinan, A. Siemens, R. Walter, W. Wright, M. Hammer- Wilson, and A. Wile, Cancer Res., 42: 2325 (1982).
F. Docchio, R. Ramponi, C. A. Sacchi, G. Bottiroli, and I. Freitas, Fluorescence studies of biological molecules by laser irradiation, in: “New Frontiers in Laser Medicine and Surgery,” K. Atsumi, ed., Excerpta Medica, Amsterdam (1983).
G. Bottiroli, I. Freitas, F. Docchio, R. Ramponi, and C. A. Sacchi, Towards a better understanding of the mechanism of action of Hematoporphyrin-Derivative at the cellular level, in: “Proc. 13th Cancer Congress,” Seattle, USA (1982).
A. Pasqua, A. Poletti, and S. M. Murgia, Med.Biol.Environ., 10: 287 (1982).
F. Docchio, R. Ramponi, C. A. Sacchi, G. Bottiroli, and I. Freitas, Time-resolved fluorescence microscopy of Hematoporphyr in-Derivative in cells, Lasers in Surgery and Medicine, 2: 21 (1982).
F. Docchio, R. Ramponi, C. A. Sacchi, G. Bottiroli, and I. Freitas, Time-resolved fluorescence microscopy of Hemato- porphyrin-Derivative in tissue- and culture-cells, in: “Laser Tokyo ’81,” K. Atsumi and N. Nimsakul, Inter Group Corp., Tokyo (1981).
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Docchio, F., Ramponi, R., Sacchi, C.A., Bottiroli, G., Freitas, I. (1985). Time-Resolved Fluorescence Microscopy: Examples of Applications to Biology. In: Martellucci, S., Chester, A.N. (eds) Laser Photobiology and Photomedicine. Ettore Majorana International Science Series, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2461-4_7
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DOI: https://doi.org/10.1007/978-1-4613-2461-4_7
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