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The Biochemical Basis of 5-Bromouracil- and 2-Aminopurine-Induced Mutagenesis

  • Myron F. Goodman
  • Randi L. Hopkins
  • Roger Lasken
  • Dhanajaya N. Mhaskar
Part of the Basic Life Sciences book series (BLSC, volume 31)

Abstract

We describe in vitro measurements of heteroduplex base mispaired intermediates involving 5-bromouracil and 2-aminopurine in A·T → G·C and G·C → A·T transition mutation pathways. For the case of 2-amino- purine, 2-aminopurine·cytosine mispairs are formed at a much higher frequency than adenine·cytosine mispairs in either transition path­way. For the case of 5-bromouracil, at least a 40-fold increase in 5-bromouracil-guanine mispairs are observed over thymine·guanine mispairs but only in the A·T → G·C pathway. In the A·T → G·C pathway, mispairs involving 5-bromouracil are formed 2.5-fold more frequently to those involving thymine suggesting perhaps that 5-bromouracil may exhibit substantially different base-pairing behavior depending on whether it is present as a template base or as a deoxyribonucleosides triphosphate substrate. The effect of the base analogs on dNTP pool size perturbations is discussed. A measurement of dNTP pools in 2-aminopurine mutagenized bacteriophage T4-infected cells is presented. An approximate eight-fold expansion in common dNTP pools is observed in a ts L141 antimutator genetic background compared to wild type T4 43+ and ts L56 mutator backgrounds. The effects of distorted dNTP pools on mutagenesis will be considered.

Keywords

Biochemical Basis Template Strand Base Analog dNTP Pool Prime Strand 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Myron F. Goodman
    • 1
  • Randi L. Hopkins
    • 1
  • Roger Lasken
    • 1
  • Dhanajaya N. Mhaskar
    • 1
  1. 1.Department of Biological Sciences Molecular Biology SectionUniversity of Southern CaliforniaLos AngelesUSA

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