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Genetic Consequences of Nucleotide Pool Imbalance pp 339–351Cite as

Mutagenesis by Incorporation of Alkylated Nucleotides

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Part of the book series: Basic Life Sciences ((BLSC,volume 31))

Summary

The cellular DNA precursor pool was shown to be a target for N-methyl-N-nitrosourea, a potent mutagen and carcinogen. O6medGTP, a product of this interaction, was chemically synthesized and shown to be incorporated into DNA in vitro by Klenow E. coli pol I and phage T4 DNA polymerases. O6medGTP incorporated predominantly opposite T template residues and to a lower extent opposite C. At some loci incorporation of O6medGTP caused DNA synthesis arrest. The significance of the behavior of O6medGTP for mutagenesis in vivo is discussed.

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Authors and Affiliations

  1. Departments of Pathology and the Cancer Research Center, University of North Carolina Medical School, Chapel Hill, North Carolina, 27514, USA

    Michael D. Topal

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  1. Michael D. Topal
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Editors and Affiliations

  1. National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA

    Frederick J. de Serres

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© 1985 Plenum Press, New York

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Topal, M.D. (1985). Mutagenesis by Incorporation of Alkylated Nucleotides. In: de Serres, F.J. (eds) Genetic Consequences of Nucleotide Pool Imbalance. Basic Life Sciences, vol 31. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2449-2_21

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  • DOI: https://doi.org/10.1007/978-1-4613-2449-2_21

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9488-7

  • Online ISBN: 978-1-4613-2449-2

  • eBook Packages: Springer Book Archive

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