Genetic Aspects of Symbiotic Nitrogen Fixation

  • Sharon R. Long
Part of the Stadler Genetics Symposia Series book series (SGSS)


Nitrogen fixation is the reduction of molecular dinitrogen (N2) to ammonia. This process, along with nitrate reduction, brings nitrogen from inorganic form into the organic world, for ammonia is the form of nitrogen which can be assimilated into amino acids. Fixed nitrogen is generated chemically by the Haber process, in which gaseous nitrogen and hydrogen are combined at high temperature and pressure to yield ammonia. This industrial process provides most of the ammonium fertilizer used in agriculture, and consumes vast amounts of petrochemical fuel every year (Postgate, 1982).


Nitrogen Fixation Host Range Nodulation Gene Rhizobium Strain Genetic Aspect 
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  1. Banfalvi, Z., Sakanyan, V., Koncz, C., Kiss, A., Dusha, I. and Kondorosi, A., 1981, Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of R. meliloti, Mol. Gen. Genet., 184:318–325.PubMedGoogle Scholar
  2. Bauer, W.D., 1981, Infection of legumes by Rhizobia, Ann. Rev. Plant Physiol., 32:407–449.CrossRefGoogle Scholar
  3. Bedmar, E.J., Edie, S.A. and Phillips, D.A., 1983, Host plant cultivar effects on hydrogen evolution by Rhizobium leguminosarum, Plant Physiol., 72 (suppl.):432.Google Scholar
  4. Beringer, J.E., Brewin, N.J., and Johnston, A.W.B., 1980, The genetic analysis of Rhizobium in relation to symbiotic nitrogen fixation, Heredity, 45:161–186.CrossRefGoogle Scholar
  5. Beynon, J.L., Beringer, J.E., and Johnston, A.W.B., 1980, Plasmids and host range in Rhizobium leguminosarumand Rhizobium phaseoli, J. Gen. Microbiol., 120:421–430.Google Scholar
  6. Bohlool, B., Kosslak, R., and Woolfenden, R., 1983, The ecology of Rhizobiumin the rhizosphere: survival, growth and competition, in:“Advances in Nitrogen Fixation,” C. Veeger and W.E. Newton, eds., pp. 287–294, Nijhoff/Junk, Boston.Google Scholar
  7. Buikema, W.J., Long, S.R., Brown, S.E., van den Bos, R., Earl, C. and Ausubel, F.M. (1983) Physical and genetic characterization of Rhizobium melilotisymbiotic mutants, J. Molec. Appl. Genet., 2:240–260.Google Scholar
  8. Callaham, D., del Tredici, P. and Torry, J.G., 1978, Isolation and cultivation in vitroof the actinomycete causing root nodulation in Comptonia., Science, 199:899–902.PubMedCrossRefGoogle Scholar
  9. Cannon, F.C., Reidel, G.E., and Ausubel, F.M., 1979, Overlapping sequences of Klebsiella pneumoniae nifDNA cloned and characterised, Molec. Gen. Genet., 174:59–66.PubMedCrossRefGoogle Scholar
  10. Corbin, D., Ditta, G., and Helinski, D., 1982, Clustering of nitrogen fixation (nif) genes in Rhizobium meliloti, J. Bacteriol. 149:221–228.PubMedGoogle Scholar
  11. Dart, P., 1974, The infection process, in“Biology of nitrogen fixation”, A. Quispel, ed., pp. 381–429, North-Holland, AmsterdamGoogle Scholar
  12. Dazzo, F.B. and Hubbell, D. 1982, Control of root hair infection. in “Nitrogen fixation, vo1.2: Rhizobium,” W. Broughton, ed., pp. 275–309, Oxford.Google Scholar
  13. Denarie, J., Boistard, P., Casse-Delbart, F., Atherley, A.G., Berry, J.O. and Russell, P., 1981, Indigenous plasmids of Rhizobium, in“Biology of the Rhizobiaceae,” K. Giles and A. Atherley, eds., pp. 225–246, Academic Press, New York.Google Scholar
  14. Ditta, G., Stanfield, S., Corbin, D., and Helinski, D., 1980, Broad host range DNA cloning system for Gram negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. 77:7347–7351.PubMedCrossRefGoogle Scholar
  15. Djordevic, M., Zurkowski, W., Shine, J., and Rolfe, B.G., 1984, Sym-plasmid transfer to various symbiotic mutants of Rhizobium trifolii, R. leguminosarum, and R. meliloti. J. Bacteriol. 156:1035–1045.Google Scholar
  16. Downie, J.A., Hombrecher, G., Ma, Q.-S., Knight, C., Wells, B., and Johnston, A.W.B., 1983, Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene, Embo J., 2:947–952.PubMedGoogle Scholar
  17. Hennecke, H., 1981, Recombinant plasmids carrying nitrogen fixation genes from Rhizobium japonicum, Nature, 291:354–355.CrossRefGoogle Scholar
  18. Imsande, J., 1981, Exchange of metabolites and energy between legume and Rhizobium, in:“Biology of the Rhizobiaceae,” K. Giles, and A. Atherly, eds., pp. 179–189, Academic Press, N.Y.Google Scholar
  19. Johnston, A.W.B., Beynon, J.L., Buchanan-Wollaston, A.V., Setchell, S.M., Hirsch, P.R., and Beringer, J.E., 1978, High frequency transfer of nodulatiog ability between strains and species of Rhizobium, Nature, 276:635–636.CrossRefGoogle Scholar
  20. Jordan, D.C., 1982, Transfer of Rhizobium japonicumto Bradyrhizobium, Intl. J. Syst. Bacteriol., 32:136–139.CrossRefGoogle Scholar
  21. Long, S.R., Meade, H.M., Brown, S.E. and Ausubel, F.M., 1981, Transposon-induced symbiotic mutants of Rhizobium meliloti, in:“Genetic engineering in the plant sciences,” N. Panopoulos, ed., pp. 129–143, Praeger, New York.Google Scholar
  22. Long, S.R., Buikema, W.E., and Ausubel, F.M., 1982, Cloning of Rhizobium melilotinodulation genes by direct complementation of Nod- mutants, Nature, 298:485–488.CrossRefGoogle Scholar
  23. Long, S.R., 1984, Nodulation genetics, in: “Plant-microbe interactions,” E. Nester and T. Kosuge, eds., Macmillan, New York, in press.Google Scholar
  24. Ma, Q.-S., Johnston, A.W.B., Hombrecher, G., and Downie, J.A., 1982, Molecular genetics of mutants of Rhizobium leguminosarum which fail to fix nitrogen, Mol. Gen. Genet., 187:166–171.CrossRefGoogle Scholar
  25. Meade, H.M., Long, S.R., Ruvkun, G.B., Brown, S.E., and Ausubel, F.M., 1982, Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium melilotiinduced by transposon Tn5mutagenesis, J. Bacteriol., 149:114–122.PubMedGoogle Scholar
  26. Newcomb, W. 1981, Nodule morphogenesis and differentiation, in“Biology of the Rhizobiaceae,” eds. Giles, K. and Atherly, A., pp. 247–298, Academic Press, New York.Google Scholar
  27. Paau, A., Leps, W. and Brill, W., 1981, Agglutinin from alfalfa necessary for binding and nodulation by Rhizobium meliloti, Science, 213:1513–1515.PubMedCrossRefGoogle Scholar
  28. Peters, G.A. and Mayne, B.C., 1974, The Azolla-Anabaena azollae relationship. I: Initial characterization of the association, Plant Physiol., 53:813–819.PubMedCrossRefGoogle Scholar
  29. Pierce, M. and Bauer, W.D., 1983, A rapid regulatory response governing nodulation in soybean, Plant Physiol, 73:286–290.PubMedCrossRefGoogle Scholar
  30. Postgate, J., 1982, “The fundamentals of nitrogen fixation,” Cambridge Press, Cambridge.Google Scholar
  31. Quinto, C., de la Vega, H., Flores, M., Fernandez, L., Ballado, T., Soberon, G., and Palacios, R., 1982, Reinteration of nitrogen fixation gene sequences in Rhizobium phaseoli, Nature299:724–726.CrossRefGoogle Scholar
  32. Robson, R., Kennedy, C., and Postgate, J., 1983, Progress in comparative genetics of nitrogen fixation, Can. J. Microbiol., 29:954–967.PubMedCrossRefGoogle Scholar
  33. Rosenberg, C., Boistard, P., Denarie, J., and Casse-Delbart, F., 1981, Genes controlling early and late function in symbiosis are located on a megaplasmid in Rhizobium meliloti. Mol. Gen. Genet., 194:326–333.Google Scholar
  34. Ruvkun, G.B. and Ausubel, F.M., 1980, Interspecies homology of nitrogenase genes, Proc. Natl. Acad. Sci. U.S.A., 77:191–195.PubMedCrossRefGoogle Scholar
  35. Ruvkun, G.B. and Ausubel, F.M., 1981, A general method for site-directed mutagenesis in prokaryotes, Nature, 289:75–78.CrossRefGoogle Scholar
  36. Ruvkun, G.B., Sundaresan, V. and Ausubel, F.M., 1982, Site-directed transposon Tn5 mutagenesis and complementation analysis of the Rhizobium melilotisymbiotic nitrogen fixation (nif) genes, Cell, 29:551–559.PubMedCrossRefGoogle Scholar
  37. Scott, K.F., Hughes, J.E., Gresshoff, P.M., Beringer, J.E., Rolfe, B.G., and Shine, J., 1982, Molecular cloning of Rhizobium trifoliigenes involved in symbiotic nitrogen fixation, J. Molec. Appl. Genet., 1:315–326.Google Scholar
  38. Sprent, J., 1979, The biology of nitrogen-fixing organisms. McGraw-Hill, London.Google Scholar
  39. Sutton, W.D., Pankhurst, C.E., and Craig, A.S., 1981, The Rhizobium bacteroid state, in:“Biology of the Rhizobiaceae,” K. Giles and A. Atherly, eds., pp. 149–177, Academic Press, New York.Google Scholar
  40. Verma, D.P.S. and Long, S.R., 1983, The molecular biology of Rhizobium-legume symbiosis, in: “Intracellular Symbioses,” K. Jeon, ed., pp. 211–243, Academic Press, New York.Google Scholar
  41. Vincent, J.M., 1980, Factors controlling the Rhizobium-legume symbiosis. in“Nitrogen fixation, vol. II: symbiotic associations and cyanobacteria,” W.E. Newton and W.H. Orme-Johnson, eds., Park Press, Baltimore.Google Scholar
  42. Zimmerman, J.L., Szeto, W. and Ausubel, F.M., 1984, Molecular characterization of Tn5-induced symbiotic (Fix-) mutants of Rhizobium meliloti, J. Bacteriol., 156:1025–1034.Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Sharon R. Long
    • 1
  1. 1.Department of Biological SciencesStanford UniversityStanfordUSA

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