Abstract
Heterogeneous as compared with homogeneous type derivatizations, either off-line or on-line, pre- or post-column, offer more advantages than disadvantages. This is especially true when solid phase reactions are fully compatible with HPLC mobile phases, and can be performed quantitatively in ‘real time’, on-line, at ambient temperatures under HPLC pressure conditions. Some polymeric or solid-supported reactions/reagents have now been developed and applied to both on-line and off-line HPLC separations, usually with UV detection. Such approaches permit the use of difference chromatography, first a dummy run without reagent and then a repeat with the solid phase reactor (SPR) on line. The comparison aids analyte identification and specificity, especially when the nature of the reaction has been well defined for particular classes of analytes.
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© 1984 Plenum Press, New York
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Krull, I.S. et al. (1984). Solid Phase Derivatization Reactions in HPLC. In: Reid, E., Wilson, I.D. (eds) Drug Determination in Therapeutic and Forensic Contexts. Methodological Surveys in Biochemistry and Analysis, vol 14. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2397-6_20
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DOI: https://doi.org/10.1007/978-1-4613-2397-6_20
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