Abstract
The cisplatin analogues investigated relate to the search for a second generation Pt complex less toxic or more active than the parent compound. For cis-diammine(1,1-cyclobutane-dicarboxylato) platinum (CBDCA), normal-phase chromatography on silica with UV detection suffices down to levels of 10 μM in urine or plasma ultrafiltrates. Quantitation below 10 μM needs HPLC fraction collection followed by flameless atomic absorption spectrophotometry (FAAS; 0.5 μM detectable). These methods have been successfully applied to CBDCA in plasma and urine from patients and rats [1, 2]. For cis-dichloro- trans -dihydroxy bis (isopropylamine) platinum (CHIP), plasma ultrafiltrates were analyzed by HPLC on a μBondapak phenyl column with UV detection [3], or by water /methanol gradient elution from a μBondapak C-18 column followed by fraction collection and FAAS [4] (detection limits 1 μM and 0.04 μM respectively). Urines containing CHIP were analyzed by the latter method [3, 5]. These assays have been successfully applied to dog and human samples where besides CHIP, cis-dichloro-bis(isopropylamine)platinum has been found [3]. [Formulae are shown overleaf.-Ed.]
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Harland, S.J., Newell, D.R., Siddik, Z.H., Chadwick, R., Calvert, A.H. & Harrap, 44. (1693) Cancer Res.44, 1693
Siddik, Z.H., Newell, D.R., Boxall, F.E., Jones, M., McGhee, K.G. & Harrap, K.R. (1984) in Platinum Coordination Complexes in Cancer Chemotherapy [Proc. IV Int. Platinum Symp., 1983](Hacker, M., Double, E. & Krakoff, I. eds.), Martinus Nijhoff, Boston, pp. 90 – 102.
Pendyala, L., Cowens, J.W., Madajewicz, S. & Creaven, P.J. (1984) as for 2., pp. 114–125.
Pendyala, L., Greco, W., Cowens, J.W., Madajewicz, S. & Creaven, P.J. (1983) Cancer Chemother. Pharmacol.11, 23 – 28.
Pendyala, L., Cowens, J.W. & Creaven, P.J. (1982) Cancer Treatm. Rep. 66, 509 – 516.
van der Vijgh, W.J.F., van der Lee, H.B.J., Postma, G.J. & Pinedo, H.M. (1983) Chromatographia 8, 333 – 336.
Kelsen, D.P., Scher, H., Alcock, N., Leyland-Jones, B.,Donner, A., Williams, L., Greene, G., Burchenal, J.H., Tan, C., Philips, F.S. & Young, C.W. (1982) Cancer Res. 42, 4831 – 4835
Harrap, K.R. (1983) in Cancer Chemotherapy I(Muggia, F.M., ed.), Nijhoff, The Hague, pp. 171 – 217.
Himmelstein, K.J., Patton, T.F., Belt, R.J., Taylor, S., Repta, A.J. & Sternson, L.A. (1981) Clin. Pharmacol. Ther. 29, 658 – 664.
Riley, C.M., Sternson, L.A., Repta, A.J. & Siegler, R.W. (1982) J. Chromatog. 229, 373 – 386.
Krull, I.S., Ding, X-D., Braverman, S., Selavka, C., Hochberg, F. & Sternson, L.A. (1983) J.Chromatog. Sci. 21, 166 – 173
Kelsen, D., Scher, H. & Burchenal, J. (1984) as for 2., pp. 310–320.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Newell, D.R., Siddik, Z.H., Harrap, K.R. (1984). HPLC Analysis of Cisplatin Analogues in Biological Fluids. In: Reid, E., Wilson, I.D. (eds) Drug Determination in Therapeutic and Forensic Contexts. Methodological Surveys in Biochemistry and Analysis, vol 14. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2397-6_17
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2397-6_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9462-7
Online ISBN: 978-1-4613-2397-6
eBook Packages: Springer Book Archive