Routes of Calcium Flux in Cardiac Sarcoplasmic Reticulum

  • Joseph J. Feher


Net release of Ca2+ from the sarcoplasmic reticulum (SR) plays an important role in coupling excitation to contraction in cardiac muscle cells, and the uptake of Ca2+ by SR plays a major role in effecting relaxation of the contractile apparatus [9,20,26]. There are several possible routes of Ca2 + influx and efflux in SR that could account for the net uptake and release of Ca2+. These putative pathways include passive diffusion, pump-mediated Ca2+ influx and efflux, carrier-mediated facilitated diffusion, and Ca2+ efflux through a gated channel. One goal of research in this field is to identify the routes of Ca2+ flux in SR and to determine their magnitude, time course, and roles in physiological regulation of Ca2+ flux. In this presentation, the routes of Ca2+ flux in SR vesicles isolated from dog hearts are examined. The results suggest that Ca2+ fluxes in cardiac SR occur through only three routes: (1) forward pump-mediated Ca2+ influx; (2) reverse pump-mediated Ca2+ efflux; and (3) passive efflux.


ATPase Activity Sarcoplasmic Reticulum Calcium Oxalate Gated Channel Release Model 
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  1. 1.
    Berman,M. C. Energy coupling and uncoupling of active calcium transport by sarcoplasmic reticulumBiochim. Biophys. Acta 694: 95–121, 1982.PubMedGoogle Scholar
  2. 2.
    Chevallier, J.; Bonnet, J.-P.; Galante, M.; Tenu, J.-P.; Gulik-Krzywicki, T. Functional and structural heterogeneity of sarcoplasmic reticulum preparationsBiol. Cell 30: 103–110, 1977.Google Scholar
  3. 3.
    Deamer, D. W.; Baskin, R. J. ATP synthesis in sarcoplasmic reticulumArch. Biochem. Biophys 153: 47–54, 1972.PubMedCrossRefGoogle Scholar
  4. 4.
    Dunnet, J.; Nayler, W. G. Effect of pH on calcium accumulation and release by isolated fragments of cardiac and skeletal muscle sarcoplasmic reticulumArch. Biochem. Biophys 198:434–438, 1979.CrossRefGoogle Scholar
  5. 5.
    Ebashi, S.; Lipmann, F. Adenosine triphosphate-linked concentration of calcium ions in a particulate fraction of rabbit muscleJ. Cell Biol 14: 389–400, 1962.PubMedCrossRefGoogle Scholar
  6. 6.
    Entman, M. L.; Van Winkle, W. B.; Bornet, E.; Tate, C. Spontaneous calcium release from sarcoplasmic reticulum: A re-examinationBiochim. Biophys. Acta 551: 382–388, 1979.PubMedGoogle Scholar
  7. 7.
    Fabiato, A. Calcium release in skinned cardiac cells: Variations with species, tissues and developmentFed. Proc 41: 2238–2244, 1982.PubMedGoogle Scholar
  8. 8.
    Fabiato, A. Fluorescence and differential light absorption recordings with calcium probes and potential- sensitive dyes in skinned cardiac cellsCan. J. Physiol. Pharmacol 60: 556–567, 1982.PubMedCrossRefGoogle Scholar
  9. 9.
    Fabiato, A.; Fabiato, F. Calcium and cardiac excitation-contraction couplingAnnu. Rev. Physiol 41: 473–484, 1979.PubMedCrossRefGoogle Scholar
  10. 10.
    Feher, J. J.; Briggs, F. N. The effect of calcium oxalate crystallization kinetics on the kinetics of calcium uptake and calcium ATPase activity of sarcoplasmic reticulum vesiclesCell Calcium 1: 105–118, 1980.CrossRefGoogle Scholar
  11. 11.
    Feher, J. J.; Briggs, F. N. The effect of calcium load on the calcium permeability of sarcoplasmic reticulumJ. Biol. Chem 257: 10191–10199, 1982.PubMedGoogle Scholar
  12. 12.
    Feher, J. J.; Briggs, F. N. Determinants of calcium loading at steady state in sarcoplasmic reticulumBiochim. Biophys. Acta 727: 389–402, 1983.PubMedCrossRefGoogle Scholar
  13. 13.
    Friedman, Z.; Makinose, M. Phosphorylation of skeletal muscle microsomes by acetyl phosphateFEBS Lett 11: 69–72, 1970.PubMedCrossRefGoogle Scholar
  14. 14.
    Guimaraes-Motta, H.; DeMeis, L. Pathway for ATP synthesis by sarcoplasmic reticulum ATPaseArch. Biochem. Biophys 203: 395–403, 1980.PubMedCrossRefGoogle Scholar
  15. 15.
    Hasselbach, W.; Makinose, M. ATP and active transportBiochem. Biophys. Res. Commun 7: 132–136, 1962.PubMedCrossRefGoogle Scholar
  16. 16.
    Hill, T. LFree Energy Transduction in Biology, New York, Academic Press, 1977.Google Scholar
  17. 17.
    Inesi, G.; Kurzmack, M.; Kosk-Kosicka, D.; Lewis, D.; Scofano, H.; Guimaraes-Motta, H. Equilibrium and kinetic studies of calcium transport and ATPase activity in sarcoplasmic reticulumZNaturforsch. Teil C 37: 685–691, 1982.Google Scholar
  18. 18.
    Jones, L. R.; Besch, H. R. Calcium handling by cardiac sarcoplasmic reticulumTex. Rep. Biol. Med 39: 19–35, 1979.PubMedGoogle Scholar
  19. 19.
    Jones, L. R.; Besch, H. R.; Sutko, J. L.; Willerson, J. T. Ryanodine-induced stimulation of net Ca uptake by cardiac sarcoplasmic reticulum vesiclesJ. Pharmacol. Exp. Ther 209: 48–55, 1979.PubMedGoogle Scholar
  20. 20.
    Levitsky, D. O.; Benevolensky, D. S.; Levchenko, T. S.; Smirnov, V. N.; Chazov, E. I. Calcium-binding rate and capacity of cardiac sarcoplasmic reticulumJ. Mol. Cell. Cardiol 13: 785–796, 1981.PubMedCrossRefGoogle Scholar
  21. 21.
    Makinose, M.; Hasselbach, W. ATP synthesis by the reverse of the sarcoplasmic calcium pumpFEBS Lett 12: 271–272, 1971.PubMedCrossRefGoogle Scholar
  22. 22.
    Martonosi, A.; Feretos, R. Sarcoplasmic reticulum. I. The uptake of Ca by sarcoplasmic reticulum fragmentsJ. Biol. Chem 239: 648–658, 1964.PubMedGoogle Scholar
  23. 23.
    Penpargkul, S. Effects of adenine nucleotides on calcium binding by rat heart sarcoplasmic reticulumCardiovasc. Res 13: 243–253, 1979.PubMedCrossRefGoogle Scholar
  24. 24.
    Pucell, A.; Martonosi, A. Sarcoplasmic reticulum. XIV. Acetylphosphate and carbamylphosphate as energy sources for Ca transportJ. Biol. Chem 246: 3389–3397, 1971.PubMedGoogle Scholar
  25. 25.
    Ronzani, N.; Migala, A.; Hasselbach, W. Comparison between ATP-supported and GTP-supported phosphate turnover of the calcium-transporting sarcoplasmic reticulum membranesEur. J. Biochem 101: 593–606, 1979.PubMedCrossRefGoogle Scholar
  26. 26.
    Solaro, J.; Briggs, F. N. Estimating the functional capabilities of sarcoplasmic reticulum in cardiac muscleCirc. Res 34: 531–540, 1974.PubMedGoogle Scholar
  27. 27.
    Stein, W. D. An algorithm for writing down flux equations for carrier kinetics, and its application to co- transportJ. Theor. Biol 62: 467–478, 1976.PubMedCrossRefGoogle Scholar
  28. 28.
    Suko, J.; Hasselbach, W. Characterization of cardiac sarcoplasmic reticulum ATP-ADP phosphate exchange and phosphorylation of the calcium transport adenosine triphosphataseEur. J. Biochem 64: 123–130, 1976.PubMedCrossRefGoogle Scholar
  29. 29.
    Suko, J.; Hellman, G.; Winkler, F. The reversal of the calcium pump of cardiac sarcoplasmic reticulumBasic Res. Cardiol 72: 147–152, 1977.PubMedCrossRefGoogle Scholar
  30. 30.
    Takakuwa, Y.; Kanazawa, T. Reaction mechanism of (Ca,Mg)-ATPase of sarcoplasmic reticulum vesiclesJ. Biol. Chem 256: 2696–2700, 1981.PubMedGoogle Scholar
  31. 31.
    Takenaka, H.; Adler, P. N.; Katz, A. M. Calcium fluxes across the membrane of sarcoplasmic reticulum vesiclesJ. Biol. Chem 257: 12649–12656, 1982.PubMedGoogle Scholar
  32. 32.
    Verjovski-Almeida, S.; Kurzmack, M.; Inesi, G. Partial reactions in the catalytic and transport cycle of sarcoplasmic reticulum ATPaseBiochemistry 17: 5006–5013, 1978.PubMedCrossRefGoogle Scholar
  33. 33.
    Waas, W.; Hasselbach, W. Interference of nucleoside diphosphates and inorganic phosphate with nucleoside triphosphate-dependent calcium fluxes and calcium-dependent nucleoside-triphosphate hydrolysis in membranes of sarcoplasmic reticulum vesiclesEur. J. Biochem 116: 601–608, 1981.PubMedCrossRefGoogle Scholar
  34. 34.
    Winkler, F.; Suko, J. Phosphorylation of the calcium transport adenosine triphosphatase of cardiac sarcoplasmic reticulum by orthophosphateEur. J. Biochem 77: 611–619, 1977.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Joseph J. Feher
    • 1
  1. 1.Department of Physiology and BiophysicsMedical College of VirginiaRichmondUSA

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