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Calcium-Activated Potassium Channels in Bullfrog Sympathetic Ganglion Cells

  • Paul R. Adams
  • David A. Brown
  • Andrew Constanti
  • Robert B. Clark
  • Leslie Satin

Abstract

The existence of a calcium activated potassium current (Ic) in the somata of vertebrate sympathetic ganglion cells was postulated to account for the calcium-sensitive spike after-hyperpolarizations present in these cells [19,22,26]. We have studied Ic in bullfrog ganglion cells more directly by using various voltage-clamp techniques, partly in order to understand better the role this current plays in spike repolarization, spike afterhyperpolarization, and spontaneous hyperpolarizations, and partly to define the difference between Ic and the M-current Im [4]. Both Ic and Im are voltage-sensitive potassium currents sensitive to transmitters, the former being activated by internal calcium and the latter inactivated by external acetylcholine. Despite these superficial similarities, it turns out that the two currents have virtually nothing in common.

Keywords

Ganglion Cell Outward Current Relaxation Time Constant Internal Calcium Dorsal Root Ganglion Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Adams, P. R. Kinetics of agonist conductance changes during hyperpolarization at frog end-plates. Br. J. Pharmacol 53: 308–310, 1975.PubMedGoogle Scholar
  2. 2.
    Adams, P. R. The calcium current of a vertebrate neurone. In: Advances in Physiological Sciences, Volume 4, J. Salanki, ed., Proc. 28th Int. Congre. Physiol. Sci. Budapest, Akademiai Kiado, 1981.Google Scholar
  3. 3.
    Adams, P. R. Activation of calcium current in bullfrog sympathetic neurones. J. Physiol. (London) Submitted for publication.Google Scholar
  4. 4.
    Adams, P. R.; Brown, D. A.; Constanti, A. M-currents and other potassium currents in bullfrog sympathetic neurones. J. Physiol. (London) 330: 537–572, 1982.Google Scholar
  5. 5.
    Adams, P. R.; Brown, D. A.; Constanti, A. Voltage clamp analysis of membrane currents underlying repetitive firing of bullfrog sympathetic neurons. In: Physiology and Pharmacology of Epileptogenic Phenomena, M. R. Klee, ed., New York, Raven Press, 1982.Google Scholar
  6. 6.
    Adams, P. R.; Constanti, A.; Brown, D. A.; Clark, R. B. Intracellular Ca2+ activates a fast voltage-sensitive K+ -current in vertebrate sympathetic neurones. Nature (London) 296: 746–749, 1982.CrossRefGoogle Scholar
  7. 7.
    Barrett, J. N.; Magleby, K. L.; Pallotta, B. S. Properties of single calcium-activated potassium channels in cultured rat muscle. J. Physiol. (London) 331: 211–230, 1982.Google Scholar
  8. 8.
    Brown, D. A.; Constanti, A.; Adams, P. R. Calcium-dependence of a component of transient outward current in bullfrog ganglion cells. Soc. Neurosci. Abstr 8: 252, 1982.Google Scholar
  9. 9.
    Hartzell, H. C.; Kuffler, S. W.; Stickgold, R.; Yoshikami, D. Synaptic excitation and inhibition resulting from direct actions of acetylcholine on two types of chemoreceptors on individual amphibian parasympathetic neurones. J. Physiol. (London) 271: 817–846, 1977.Google Scholar
  10. 10.
    Henkart, M. Indentification and function of intracellular calcium stores in axons and cell bodies of neurons. Fed. Proc. 39: 2783 - 2789, 1980.PubMedGoogle Scholar
  11. 11.
    Henkart, M.; Landis, D. M. D.; Reese, T. S. Similarity of junctions between plasma membranes and endoplasmic reticulum in muscle and neurons. J. Cell Biol 70: 388–347, 1976.CrossRefGoogle Scholar
  12. 12.
    Hermann, A.; Hartung, K. Noise and relaxation measurements of the Ca2+ activated K+ current in Helix neurones. Pfluegers Arch. 393: 254 - 261, 1982.CrossRefGoogle Scholar
  13. 13.
    Latorre, R.; Vergara, C.; Hidalgo, C. Reconstitution in planar lipid bilayers of a Ca2+ -dependent K+ channel from transverse tubule membranes isolated from rabbit skeletal muscle, Proc. Natl. Acad. Sci. USA 79: 805–809, 1982.PubMedCrossRefGoogle Scholar
  14. 14.
    Lee, K. S.; Tsien, R. W. Reversal of current through calcium channels in dialysed single heart cells. Nature (London) 297: 498–501, 1982.CrossRefGoogle Scholar
  15. 15.
    MacDermott, A. B.; Weight, F. F. Action potential repolarization may involve a transient Ca2+ -sensitive outward current in a vertebrate neurone. Nature (London) 300: 185 - 188, 1982.CrossRefGoogle Scholar
  16. 16.
    Madison, D. V.; Nicoll, R. A. Noradrenaline blocks accommodation of pyramidal cell discharge in hippocampus. Nature (London) 299: 636–638, 1982.CrossRefGoogle Scholar
  17. 17.
    Marty, A. Ca-dependent K channels with large unitary conductance in chromaffin cell membranes. Nature (London) 291: 497–500, 1981.CrossRefGoogle Scholar
  18. 18.
    Mathers, D. A.; Barker, J. L. Spontaneous hyperpolarizations at the membrane of cultured mouse dorsal root ganglion cells. Brain Res. 211: 451–455, 1981.PubMedCrossRefGoogle Scholar
  19. 19.
    McAfee, D. A.; Yarowsky, P. J. Calcium-dependent potentials in the mammalian sympathetic neurone. J. Physiol. (London) 290: 507–523, 1974.Google Scholar
  20. 20.
    Meech, R. W.; Standen, N. B. Potassium activation in Helix aspersa neurones under voltage clamp: A component mediated by calcium influx. J. Physiol. (London) 249: 211–239, 1975.Google Scholar
  21. 21.
    Methfessel, C.; Boheim, G. The gating of single calcium-dependent potassium channels is described by an activation/blockade mechanism. Biophys. Struct. Mech 9: 35–60, 1982.PubMedCrossRefGoogle Scholar
  22. 22.
    Minota, S. Calcium ions and the post-tetanic hyperpolarization of bulffrog sympathetic ganglion cells. Jpn. J. Physiol 24: 501–512, 1974.Google Scholar
  23. 23.
    Morita, K.; Koketsu, K.; Kuba, K. Oscillation of [Ca2+] linked K+ conductances in bullfrog sympathetic ganglion cell is sensitive to intracelhilar axons. Nature (London) 283: 204–205, 1980.CrossRefGoogle Scholar
  24. 24.
    Morita, K.; North, R. A.; Tokimasa, T. Muscarinic agonists inactivate potassium conductance of guinea pig myenteric neurones. J. Physiol. (London) 333: 125–139, 1982.Google Scholar
  25. 25.
    Neher, E.; Sakmann, B. Voltage-dependence of drug-induced conductance in frog neuromuscular junction. Proc. Natl. Acad. Sci. USA 72: 2140–2144, 1975.PubMedCrossRefGoogle Scholar
  26. 26.
    North, R. A. The calcium-dependent slow afterhyperpolarization in myenteric plexus neurones with tetrodotox- in-resistant action potentials. Br. J. Pharmacol 49: 709–711, 1973.PubMedGoogle Scholar
  27. 27.
    Sheridan, R.; Lester, H. A. Rates and equilibrium at acetylcholine receptor of electrophorus electroplaques: A study of neurally evoked postsynaptic currents and of voltage-jump relaxations. J. Gen. Physiol 70: 187–219, 1977.PubMedGoogle Scholar
  28. 28.
    Smith, S. J.; MacDermott, A. B.; Weight, F. F. Intracellular calcium transients elicited by synaptic and electrical membrane activation and by theophylline measured in bullfrog neurons using arsenazo III. Soc. Neurosci. Abstr 7: 15, 1981.Google Scholar
  29. 29.
    Tillotson, D. Inactivation of Ca conductance is dependent on entry of Ca ions in molluscan neurons. Proc. Natl. Acad. Sci. USA 76: 1497–1500, 1979.PubMedCrossRefGoogle Scholar
  30. 30.
    Wong, B. S.; Lecar, H.; Adler, M. Single calcium-dependent potassium channels in clonal anterior pituitary cells. Biophys. 7. 39: 313–317, 1982.CrossRefGoogle Scholar
  31. 31.
    Wong, F. Nature of light-induced conductance changes in ventral photoreceptors of Limulus. Nature (London) 275: 76–79, 1978.CrossRefGoogle Scholar
  32. 32.
    Woolum, J. C.; Gorman, A. L. F. Time dependence of the calcium-activated potassium current. Biophys. J 36: 297–302, 1981.PubMedCrossRefGoogle Scholar
  33. 33.
    Yau, K. W.; Lamb, D. A.; Baylor, D. A. Light-induced fluctuations in membrane current of single toad rod outer segments. Nature, (London) 269: 78–80, 1977.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Paul R. Adams
    • 1
  • David A. Brown
    • 2
  • Andrew Constanti
    • 2
  • Robert B. Clark
    • 3
  • Leslie Satin
    • 1
  1. 1.Department of Neurobiology and BehaviorState University of New YorkStony BrookUSA
  2. 2.Department of PharmacologySchool of PharmacologyLondonEngland
  3. 3.Department of Physiology and BiophysicsUniversity of Texas Medical BranchGalvestonUSA

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