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Calcium and Diacylglycerol

Separable and Interacting Intracellular Activators in Human Platelets

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Book cover Calcium in Biological Systems

Abstract

Platelet responses to many agonists involve rapid stimulation of calcium fluxes and turnover of phosphoinositides. A rise in cytoplasmic free calcium, [Ca2+]i, has been regarded as the final common pathway for shape change, secretion, and aggregation; phosphoinositide turnover has been viewed mainly as a means of mobilizing calcium [and as one route for releasing arachidonate for formation of thromboxane A2 (TxA2)]. However, much of the evidence is indirect and interpretation necessarily speculative. Two recent developments have allowed a more informed analysis of second messenger pathways.

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References

  1. Berridge, M. J. 5-Hydroxytryptamine stimulation of phosphatidylinositol hydrolysis and calcium signalling in the blowfly salivary glandCell Calcium 3: 385–397, 1982.

    Article  PubMed  CAS  Google Scholar 

  2. Castagna, M.; Takai, Y.; Kaibuchi, K.; Sano, K.; Kikkawa, U.; Nishizuka, Y. Direct activation of calcium- activated, phospholipid-dependent protein kinase by tumour-promoting phorbol estersJ. Biol. Chem 257: 7847–7851, 1982.

    PubMed  CAS  Google Scholar 

  3. Chiang, T. M.; Cagen, L. M.; Kang, A. H. Effects of 12-O-tetradecanoyl phorbol 13-acetate on platelet aggregationThromb. Res 21: 611–622, 1981.

    Article  PubMed  CAS  Google Scholar 

  4. Chiang, T. M.; Cagen, L. M.; Kang, A. H. Effects of 12-O-tetradecanoyl phorbol 13-acetate on platelet aggregationThromb. Res 21: 611–622, 1981.

    Article  PubMed  CAS  Google Scholar 

  5. Donolly, T. E.; Jensen, R. Activation of calcium-stimulated, phospholipid protein kinase by tumour-promoting phorbol ester does not require calciumFed. Proc 42: 783, 1983.

    Google Scholar 

  6. Feinman, R. D.; Detwiler, T. C. Platelet secretion induced by divalent cation ionophoresNature (London) 249: 172–173, 1974.

    Article  CAS  Google Scholar 

  7. Feinstein, M. B.; Fraser, C. Human platelet secretion and aggregation induced by calcium ionophoresJ. Gen. Physiol 66: 561–581, 1975.

    Article  PubMed  CAS  Google Scholar 

  8. Gerrard, J. M.; White, J. G.; Rao, G. H. R. Effects of the ionophore A23187 on blood platelets. II. Influence on ultrastructureAm. J. Pathol 77: 151–166, 1974.

    PubMed  CAS  Google Scholar 

  9. Hallam, T. J.; Rink, T. J.; Sanchez, A. Effects of arachidonate and a thromboxane A2 analogue on human platelets studied with a fluorescence indicator for cytoplasmic free calciumJ. Physiol. (.London) 343: 97–98, 1983.

    Google Scholar 

  10. Harrington, C. A.; Eichberg, J. Norepinephrine causes a 1-adrenergic receptor-mediated decrease of phos-phatidylinositol in isolated rat liver plasma membranes supplemented with cytosolJ. Biol. Chem 258: 2087–2090, 1983.

    PubMed  CAS  Google Scholar 

  11. Haslam, R. J.; Lynham, J. A. Relationship between phosphorylation of blood platelet proteins and secretion of platelet granule constituents. I. Effects of different aggregatory agentsBiochem. Biophys. Res. Commun 77: 714–722, 1977.

    Article  PubMed  CAS  Google Scholar 

  12. Holmes, R. P.; Yoss, N. L. Failure of phosphatidic acid to translocate calcium across phosphatidylcholine membranesNature 305: 637–638, 1983.

    Article  PubMed  CAS  Google Scholar 

  13. Kaibuchi, K.; Takai, Y.; Nishizuka, Y. Cooperative roles of various membrane phospholipids in the activation of calcium-activated, phospholipid-dependent protein kinaseJ. Biol. Chem 256: 7146–7149, 1981.

    PubMed  CAS  Google Scholar 

  14. Kaibuchi, K.; Sano, K.; Hoshijima, M.; Takai, Y.; Nishizuka, Y. Phosphatidylinositol turnover in platelet activation; calcium mobilization and protein phosphorylationCell Calcium 3: 323–335, 1982.

    Article  PubMed  CAS  Google Scholar 

  15. Knight, D. E.; Hallam, T. J.; Scrutton, M. C. Agonist selectivity and second messenger concentration in Ca2+ -mediated secretionNature (London) 296: 256–257, 1982.

    Article  CAS  Google Scholar 

  16. Lee, T. C.; Malone; B.; Blank, M. L.; Snyder, F. l-Alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) stimulates calcium influx in rabbit plateletsBiochem. Biophys. Res. Commun 102: 1262–1268, 1981.

    Article  PubMed  CAS  Google Scholar 

  17. Maclntyre, D. E.; and Pollock, W. K. Platelet-activating factor, U44069 and vasopressin stimulate phos-phatidylinositol turnover in human blood plateletsBr. J. Pharmacol 77:466P, 1982.

    Google Scholar 

  18. Massini, P.; Luscher, E. F. On the significance of influx of Ca2+ ions into stimulated human blood plateletsBiochim. Biophys. Acta 435: 652–663, 1976.

    Google Scholar 

  19. Mori, T.; Takai, Y.; Minakuchi, B. Y.; Nishizuka, Y. Inhibitory action of chlorpromazine, dibucaine, and other phospholipid-interacting drugs on calcium-activated, phospholipid-dependent protein kinaseJ. Biol. Chem 255: 8378–8380, 1980.

    PubMed  CAS  Google Scholar 

  20. Owen, N. E.; Feinberg, H.; and Le Breton, G. C. Epinephrine induces Ca2+ uptake in human blood plateletsAm. J. Physiol 239: H483 - H488, 1980.

    PubMed  CAS  Google Scholar 

  21. Pozzan, T.; Arslan, P.; Tsien, R. Y.; Rink, T. J. Anti-immunoglobulin, cytoplasmic free calcium, and capping in B-lymphocytesJ. Cell Biol 94: 335–340, 1982.

    Article  PubMed  CAS  Google Scholar 

  22. Putney, J. W., Jr. Inositol lipids and cell stimulation in mammalian salivary glandCell Calcium 3: 369–383, 1982.

    Article  PubMed  CAS  Google Scholar 

  23. Rao, G. H. R.; White, J. G. Influence of esterase inhibitors on platelet aggregation and release induced by phorbol myristate acetate. Biochem. Pharmacol. 24: 293 - 295, 1974.

    Google Scholar 

  24. Rink, T. J.; Smith, S. W.; Tsien, R. Y. Cytoplasmic free calcium in human blood platelets: Calcium thresholds and calcium-independent activation for shape-change and secretionFEBS Lett 148: 21–26, 1982.

    Article  PubMed  CAS  Google Scholar 

  25. Rittenhouse, S. E. Inositol lipid metabolism in the responses of stimulated platelets. Cell Calcium 3: 311—322, 1982.

    Google Scholar 

  26. Sanchez, A.; Hallam, T. J.; Rink, T. J. Trifluoperazine and chlorpromazine block secretion from human platelets evoked at basal cytoplasmic free calcium by activators of C-kinaseFEBS Lett 164: 43 - 46, 1983.

    Article  PubMed  CAS  Google Scholar 

  27. Siess, W.; Cuatrecasas, P.; Lapetina, E. G. A role for cyclooxygenase products in the formation of phos-phatidic acid in stimulated human plateletsJ. Biol. Chem 258: 4683–4686, 1983.

    PubMed  CAS  Google Scholar 

  28. Smith, S. W. Intracellular free calcium and human platelet activation. M.Sc. thesis, University of Cambridge, 1982.

    Google Scholar 

  29. Tsien, R. Y. New calcium indicators and buffers with high selectivity against magnesium and protons: Design, synthesis and properties of prototype structuresBiochemistry 19: 2396–2404, 1980.

    Article  PubMed  CAS  Google Scholar 

  30. Tsien, R. Y. A non-disruptive technique for loading calcium buffers and indicators into cellsNature (London) 290: 527–528, 1981.

    Article  CAS  Google Scholar 

  31. Tsien, R. Y.; Pozzan, T.; Rink, T. J. Calcium haemostasis in intact lymphocytes: Cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicatorJ. Cell Biol 94: 325–334, 1982.

    Article  PubMed  CAS  Google Scholar 

  32. Zucker, M. B.; Troll, W.; Belman, S. The tumour-promoter phorbol ester (12-0-tetradecanoyl-phorbol-13- acetate), a potent aggregating agent for blood plateletsJ. Cell Biol 60: 325–336, 1974.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Physiology Laboratory, University of Cambridge, Cambrdige, CB2 3EG, England

    T. J. Rink, A. Sanchez & T. J. Hallman

  2. Department of Physiology—Anatomy, University of California, Berkeley, California, 94720, USA

    R. Y. Tsien

Authors
  1. T. J. Rink
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  2. R. Y. Tsien
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  3. A. Sanchez
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  4. T. J. Hallman
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Editor information

Editors and Affiliations

  1. Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia, USA

    Ronald P. Rubin  & James W. Putney Jr.  & 

  2. CIBA-GEIGY Corporation, Summit, New Jersey, USA

    George B. Weiss

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© 1985 Plenum Press, New York

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Rink, T.J., Tsien, R.Y., Sanchez, A., Hallman, T.J. (1985). Calcium and Diacylglycerol. In: Rubin, R.P., Weiss, G.B., Putney, J.W. (eds) Calcium in Biological Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2377-8_18

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  • DOI: https://doi.org/10.1007/978-1-4613-2377-8_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9453-5

  • Online ISBN: 978-1-4613-2377-8

  • eBook Packages: Springer Book Archive

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