Skip to main content
SpringerLink
Log in
Book cover

African Swine Fever pp 51–61Cite as

Characterization of ASFV Proteins

  • Chapter

Part of the book series: Developments in Veterinary Virology ((DVVI,volume 3))

Abstract

The analysis of polypeptides made in cells infected with African swine fever virus (ASFV) by high-resolution polyacrylamide gel electrophoresis revealed the synthesis of at least 44 polypeptides ranging in molecular weight from 9.5 K to 243 K. Most of the proteins are synthesized within the first eight hours after infection and can be classified as immediate-early, early or late proteins. Some are modified by the incorporation of prosthetic groups: of these, at least eight were phosphorylated, and three specific viral glycoproteins were detected by immunoprecipitation. The polypeptides are localized in the nucleus and cytoplasm of infected cells, but their function is unknown. They were classified as structural and nonstructural polypeptides on the basis of their mobility in relation to the virion. Of the 28 polypeptides identified in intracellular virus, 23 are structural polypeptides. The other five could be cell polypeptides associated with the virus,as is the case with VP42 identified as cell actin. At least 34 polypeptides have been identified in extracellular virus where most of the major structural polypeptides correspond to those of the intracellular virus. The localization of these polypeptides in the virus was achieved by treatment with detergents and NaCl. No polypeptide that induces neutralizing antibodies has been found, but the major structural protein (VP73) could be important as an effective antigen in ELISA studies in the control of the disease.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Hess, W.R. Adv Vet. Sci. Comp.Med. 25, 39–69, 1981.

    PubMed  CAS  Google Scholar 

  2. Sanchez Botija, C. Rev. Sci. tech. Off. int Epiz. 1, 991–1029, 1982.

    Google Scholar 

  3. Wardley,R.C, Andrade, C.M., Black, D.N., Castro Portugal, F.L., Enjuanes, L., Hess, W.R., Mebus, C., Ordas, A., Rutili, D, Sanchez Vizcaino, Y., Vijario, Y.D. and Wilkinso P. J. Arch Virol. 76, 73–90, 1983.

    Article  CAS  Google Scholar 

  4. Vinuela, E. Curr. Top. Microbiol. Immunol. 116, 151–170, 1985.

    PubMed  CAS  Google Scholar 

  5. Matthews, R.E.F. Intervirol. 12, 129–296, 1979.

    Article  CAS  Google Scholar 

  6. De Boer, C.J., Pan, I.C. and Hess, W.R. J.A.V.M.A. 160, 528–522, 1972.

    Google Scholar 

  7. Ruiz Gonzalvo, F.R., Carnero, M.E. and V. Brugel. Comm. Eur. Comm. EUR 8466 EM, 206–216, 1982.

    Google Scholar 

  8. Bommeli, W.,Kihm, U. and F. Ehrensperger. Coom. Eur. Comm. EUR 8466 EN, 217–223, 1983.

    Google Scholar 

  9. Tabares, E., Fernandez, M.,Salvador Temprano, E., Carnero, M.E. and Sanchez Botija, C. Arch. Virol. 70, 297–300, 1981.

    Article  PubMed  CAS  Google Scholar 

  10. Black, D.N. and Brown, F. Y. gen. Virol. 32, 509–518, 1976.

    Article  CAS  Google Scholar 

  11. Vigario, J.D., Castro Portugal, F.L., Ferreira, C.A. and Festas, M.B. Comm. Europ. Comm. EUR 5904 En, 469–482, 1977.

    Google Scholar 

  12. Sanchez Botija, C., Mc Auslan, B.R., Tabares, E., Wilkinson, P., Ordas, A.Friedman, A., Solana, A., Ferreira, C.,Ruiz-Gonsalvo, F., Dalsgaard,C., Marcotegui,M.A, Becker, Y. and Schlomai, Y. Comm. Europ. Communities. EUR 5626e, 1977.

    Google Scholar 

  13. Tabares, E., Marotegui, M.A., Fernando, M. and Sanchez Botija, C. Arch. Virol. 66, 107–117, 1980.

    Article  PubMed  CAS  Google Scholar 

  14. Carrascosa, A., del Val, M., Santaren, J.F. and E. Vinuela. J. Virol. 54, 337–344, 1985.

    PubMed  CAS  Google Scholar 

  15. Tabares, E., Gonzalvo, F.R., Marcotegui, M.A. and Ordas, A. Comm. Europ. Comm. EUR 590 EN, 507–531, 1977.

    Google Scholar 

  16. Tabares, E., Martinez, J., Martin, E. and Escribano, J.M. Arch. Virol. 77, 167–180, 1983.

    Article  PubMed  CAS  Google Scholar 

  17. Sanz, A., Garcia Barreno, B., Nogal, M.L., Vinuela, E. and Enjuanes,L. J. Virol. 54, 199–206, 1985.

    PubMed  CAS  Google Scholar 

  18. Carrascosa, J.L., Caraso, J.M., Carrascosa, A.L., Garcia, N., Santesteban A. and E. Vinuela. Virol. 132, 160–172, 1984.

    Article  CAS  Google Scholar 

  19. Whvard, T.C., Wool, S.H. and Letehworth, G. J.Virol. 142, 416–420, 1985.

    Article  Google Scholar 

  20. Payne, L. J.Virol. 27, 28–37, 1978.

    PubMed  CAS  Google Scholar 

  21. Kuznar, J., Salas, M.L. and Vinuela, E. Virol. 101, 169–175, 1980.

    Article  CAS  Google Scholar 

  22. Salas, M.L., Kuznar, J. and Vinuela, E. Virol. 113, 484–49I, 1981.

    Article  CAS  Google Scholar 

  23. Polsan, A. Prep. Biochem. 4, 435–456, 1974.

    Article  Google Scholar 

  24. Salas, M.L., Kuznar, J. and Vinuela, E. Arch. Virol. 77, 77–80, 1983.

    Article  PubMed  CAS  Google Scholar 

  25. Polatnick, J., Pan, I.C. and Gravell, M. Arch. ges. Virusforsch 44, 156–159, 1974.

    Article  PubMed  CAS  Google Scholar 

  26. De Boer, C.J. Arch ges. Virusforsch 20, 164–179, 1967.

    Article  PubMed  Google Scholar 

  27. Tabares, E., Fernandez, M., Salvador-Temprano, E., Carnero, M.E. and Sanchez Botija, C. Arch Virol. 70, 297–300, 1981.

    Article  PubMed  CAS  Google Scholar 

  28. Tabares, E., Fernandez, M., Salvador Temprano, E., Carnero, M.E. and Sanchez Botija, C. C. mm. Europ. Comm. EUR 8466 EN 224–234, 1983.

    Google Scholar 

  29. Tabares, E., Martinez, J., Ruiz Gonzalvo, F. and Sanchez Botija C. Arch. Virol. 66, 119–132, 1980.

    Article  PubMed  CAS  Google Scholar 

  30. Bablanian, R. Comprehensive Virol. 19, 391–429, 1984.

    CAS  Google Scholar 

  31. Enjuanes, L., Carrascosa, A.L. and Vinuela, E. J. gen. Virol. 32, 479–492, 1976.

    Article  PubMed  CAS  Google Scholar 

  32. Ortin, J., Enjuanes, L. and Vinuela, E. J. Virol. 31, 579–583, 1979.

    PubMed  CAS  Google Scholar 

  33. Escribano, J.M. and E. Tabares (Submitted to publication).

    Google Scholar 

  34. Escribano, J.M. and E. Tabares. Comm. Europ. Comm., 1984,in press.

    Google Scholar 

  35. Tabares, E., Ruiz Gonzalvo, F. and Carnero, M.E. Libro jubilar prof. C. Sanchez Botija. Ed. Fareiso. Madrid 1983.

    Google Scholar 

  36. Letehworth, G.J. and Whyard, T.C. Arch Virol. 80, 265–274, 1984.

    Article  Google Scholar 

  37. Moura Nunes, J.F., Vigario, J.D. and Terrhinha, A.M. Arch. Virol. 49, 59–66, 1975.

    Article  Google Scholar 

  38. Breese, S.S. and De Boer, C.J. Virol. 28, 420–428, 1966.

    Article  Google Scholar 

  39. Moreno, M.A., Carrascosa, A.L., Ortin, J. and Vinuela, E. J. gen. Virol. 93, 253–258, 1978.

    Article  Google Scholar 

  40. Gil Fernandez, C., Paez, E., Vilas, P. and Garcia Gancedo. Chemotherapy 25, 162–169, 1979.

    Article  PubMed  CAS  Google Scholar 

  41. Tabares,,.E. and Sanchez Botija., C. Arch. Virol. 61, 49–59, 1979.

    Article  PubMed  CAS  Google Scholar 

  42. Ortin, J. and Vinuela, E. J. Virol. 21, 902–905, 1977.

    PubMed  CAS  Google Scholar 

  43. Moura Nunes, J.F., Vigario, J.D. and Terrinha, A.M. Arch. Virol. 49, 59–66, 1975.

    Article  Google Scholar 

  44. Pan, I.C., Schimizu, M. and Hess, W.R Amer. J. Vet. Res: 41, 1357–1367, 1980.

    PubMed  CAS  Google Scholar 

  45. Polatrick, J. and Hess, W.R. Arch. ges. Viresforch 38, 383–385, 1972.

    Article  Google Scholar 

  46. Polatrick, J. and Hess, W.R. Amer. J. Vet. Res. 31, 1609–1613, 1970.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Microbiologia, Facultad de Medicina, Universidad Autonoma de Madrid, Arzobispo Morcillo 4, Madrid, 28029, Spain

    Enrique Tabares

Authors
  1. Enrique Tabares
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Molecular Biology Faculty of Medicine, The Hebrew University of Jerusalem, P.O. Box 1172, 91010, Jerusalem, Israel

    Yechiel Becker

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Martinus Nijhoff Publishing, Boston

About this chapter

Cite this chapter

Tabares, E. (1987). Characterization of ASFV Proteins. In: Becker, Y. (eds) African Swine Fever. Developments in Veterinary Virology, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2343-3_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-2343-3_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9437-5

  • Online ISBN: 978-1-4613-2343-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation