In Vivo Kinetics in Autologous Transfusion of Red Cells Preserved 42 and 49 Days at + 4°C in Paggss and in Adsol-AS1

Abstract

Extending the storage period of red blood cells at + 4°C will help to improve the supply in some countries and will simplify the development of autologous transfusion programmes. Transfused red blood cells (RBC) are expected to act as efficient substitutes; this implies the same capacity to bring oxygen to the tissues, and the same life span as normal red blood cells. The control of these properties is mandatory to the validation of new red cell preservation procedures. As the oxygen dissociation curve is a function of the 2,3 DPG level which will be regenerated if needed after 12 to 48 hours in the circulation [1], the RBC ability to actually deliver oxygen to the tissues depends on the red cells capacity to survive in the circulation. We studied the in-vivo kinetics of RBC stored at + 4°C in ADSOL-AS1 (Fenwal Laboratories) and PAGGSS (Biotest Laboratories) for 42 and 49 days in autologous transfusion. PAGGSS and ADSOL-AS1 are two optional additive solutions according to the concept pioneered by Lovric and Högman [2,3]. ADSOL-AS1 is a variant of Högman’s SAG-Mannitol [4] and PAGGSS contains phosphate adenine guanosine glucose saline and sorbitol according to Spielman and Seidl [5].