Neovascularisation of intracranial tumours
Extracts from samples of 1 glioblastoma, 8 meningiomas (5 of which were pooled) and pooled samples of 4 acoustic nerve tumours were tested for endothelial-cell-stimulating angiogenesis factor (ESAF). The ex-tracts were separated into fractions of known molecular mass using gel-filtration chromatography. Neo-vascularisation resulting from angiogenic activity was assessed for each fraction using the chick chorioallan-toic (CAM) and chick vitelline membrane (VIM) tests. Angiogenic activity was found in all the samples tested. The activity was present in the dialysate, suggesting that the factor responsible was of low Mr. Some samples were further fractionated on Biogel P2 and activity was demonstrated in fractions of molecular mass 300–600. ESAF activity was easily dissociated from any carrier protein which may have been present. ESAF from other sources has been shown previously to activate fibroblast procollagenase and the meningioma ESAF had the same ability.
Keywordsangiogenesis endothelial cells intracranial neoplasm neovascularisation tumour angiogenesis factor
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