Limitations and Potentials of In Vitro Sensitivity Testing of Human Small Cell Carcinoma of the Lung
The clonogenic assay has been proposed both as a tool for individually guided chemotherapy and as a preclinical drug evaluation system. Because tumor cells are heterogeneous it is unlikely that a test where only a minor fraction of the cells is tested can predict the sensitivity of all tumor cells in the patient. The pronounced negative accuracy reported can be explained on the basis of the patient populations being studied. The technical limitations of the test are presented, and it is concluded that with the present status of the test, it is only suited as a preclinical test system.
It is suggested that in vitro sensitivity testing of drugs in a panel of cell lines established from patients with small cell carcinoma of the lung might help to identify drugs that could alter the clinical course of the disease.
KeywordsClonogenic Assay Sensitivity Pattern Continuous Cell Line Human Small Cell Lung Cancer Human Tumor Stem Cell
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