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Adenovirus DNA pp 343-366 | Cite as

Expression of Class I Major Histocompatibility Antigens in Adenovirus-Transformed Cells

  • A. J. van der Eb
  • B. A. Oostra
Part of the Developments in Molecular Virology book series (DMVI, volume 8)

Abstract

Human adenoviruses have been used extensively as a model for studies on the mechanism of oncogenic transformation. The interest in this group of viruses is due to the fact that they combine a number of unique features, that are not found in other viruses: (a) Oncogenic transformation by adenoviruses is the result of the activity of 2 or 3 distinct viral gene products, rather than by the action of a single viral protein, as in the case of SV40. This seemingly greater complexity of the transforming region of adenovirus is an advantage rather than a disadvantage. It should considerably simplify genetic and functional analysis of transformation as compared to the situation in SV40, where the transforming functions are all compressed into a single multifunctional protein (1). (b) Oncogenic transformation by adenoviruses can be considered to be a “multistep” process in which 2 or 3 genes are involved (2). This resembles the situation in spontaneous cancer in animals, which also requires the concerted activity of two, or possibly more, cellular oncogenes. Interestingly, a functional similarity has been established between the adenovirus E1A and E1B regions on one hand, which together constitute the viral transforming region, and the two cooperating cellular oncogenes myc and ras on the other (3,4). (c) The human adenoviruses can be divided into oncogenic and non-oncogenic serotypes (e.g. Ad 12, an oncogenic virus, and Ad2 or Ad5, both non-oncogenic viruses). Despite this difference in biological behavior, all human adenoviruses tested so far are capable of morphologically transforming cultured cells. The structural and organizational relatedness of the Ad5 and Ad 12 transforming regions implies that the difference in oncogenicity must be brought about by one or more subtle differences in gene function, and may enable us to dissociate oncogenicity, i.e. the ability to grow as a tumor in normal animals, from morphological transformation in vitro.

Keywords

Natural Killer Cell Adenovirus Type Human Adenovirus Ad12 Region Allogeneic CTLs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishing, Boston 1986

Authors and Affiliations

  • A. J. van der Eb
    • 1
  • B. A. Oostra
    • 1
  1. 1.Sylvius LaboratoriesUniverity of LeidenLeidenThe Netherlands

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