Glial Cell Lineages and Differentiation in the Rat Optic Nerve

  • Martin C. Raff


My colleagues and I are interested in the problem of cell diversification in the mammalian central nervous system (CNS): how does the relatively simple neuroepithelium of the embryonic neural tube give rise to the large numbers of different types of neurons and glial cells of the adult CNS? To overcome the technical problems associated with cell identification, inaccessibility to experimental manipulation and the intimidating cellular complexity of the CNS, we have employed three strategies: (i) we have used antibodies to identify and manipulate specific types of neural cells and their precursors; (ii) we have studied cell diversification in culture so that we can manipulate the cells and their environment, and (iii) we have studied one of the simplest parts of the CNS, the rat optic nerve, which contains astrocytes and oligodendrocytes but no intrinsic neurons, so that glial cell development can be studied without the complication of neuronal development. Rather than review the experiments we have done, many of which have been published, I will briefly summarize some of the conclusions we have drawn from our studies so far.


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  1. 1.
    M.C. Raff, E.R. Abney, J. Cohen, R. Lindsay and M. Noble, Two types of astrocytes in cultures of developing rat white walker. Differences in morphology surface gangliosides and growth characteristics. J. Neurosci., 3: 1289 (1983).PubMedGoogle Scholar
  2. 2.
    R.H. Miller, S. David, R. Patel, E.R. Abney, and. M.C. Raff, A quantitative immunohistochemical study of microglial cell development in the rat optic nerve: in vivo evidence for two distinct astrocyte lineages. Dev. Biol., 111: 35 (1985).PubMedCrossRefGoogle Scholar
  3. 3.
    M.C. Raff, E.R. Abney and R.H. Miller, Two glial ceil lineages diverge prenatally in rat optic nerve. Dev. Biol., 106: 53 (1984).PubMedCrossRefGoogle Scholar
  4. 4.
    M.C. Raff, R.H. Miller and M. Noble, A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium, Nature, 303: 390 (1983)PubMedCrossRefGoogle Scholar
  5. 5.
    P. Skoff, D.L. Price and A. Stocks, Electron microscope autoradiographic studies of gliogenesis in rat optic nerve. II Time of origin. J. Comp. Neurol., 169: 313. (1976).Google Scholar
  6. 6.
    S. Temple and M.C. Raff, Differentiation of a bipotential glial progenitor cell in single cell microculture, Nature, 313: 223 (1985).PubMedCrossRefGoogle Scholar
  7. 7.
    M.C. Raff, E.P. Williams and R.H. Miller, The in vitro differentiation of a bipotential glial progenitor cell, EMBO J., 3: 1857 (1984).PubMedGoogle Scholar
  8. 8.
    M. Noble and K. Hurray, Purified astrocytes promote the in vitro division of a bipotential glial progenitor cell, EMBO J., 3: 2243 (1984).PubMedGoogle Scholar
  9. 9.
    J. Fok-Seang, E.R. Abney and M.C. Raff, in preparation.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Martin C. Raff
    • 1
  1. 1.Medical Research Council Neuroimmunology Project Zoology DepartmentUniversity College LondonLondonUK

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