Abstract
In 1980 we reported that there is a sodium dodecyl sulfate (SDS)-insoluble filamentous protein in Physarum, and this peculiar protein is very similar in filamentous structure and amino acid composition to connecting an elastic protein of striated muscle (Ozaki and Maruyama, 1980). Recently, Gassner and his colleagues at the University of Bonn have been able to isolate a similar protein in an SDS-soluble form and have characterized it from the viewpoint of a new cytoskeletal matrix in the Plasmodium (Gassner et al., 1985; see Chapter 15). We have also separated a similar connectin-like protein in the presence of SDS; however, all the preparations mentioned above were denatured proteins, and, therefore, its physiological function was not studied at all except for its electron microscopic structure.
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References
Ozaki, K., and Maruyama, K., 1980, A connectin-like protein from the Plasmodium Physarum plasmodium, J. Biochem ., 88: 883.
Gassner, D., Shraideh, Z., and Wohlfarth-Bottermann, K.-E., 1985, A giant titin-like protein in Physarum polycephalum; evidence for its candidacy as a major component of an elastic cytoskeletal superthin filament lattice, Eur. J. Cell Biol ., 37: 44.
Kimura, S., Maruyama, K., and Huang, Y. P., 1984, Interactions of muscle β-connectin with myosin, actin, and actomyosin at low ionic strengths, J. Biochem ., 96: 499.
Maruyama, K., Kimura, S., Yoshidomi, H., Sawada, H., and Kikuchi, M., 1984, Molecular size and shape of β-connectin, an elastic protein of striated muscle, J. Biochem ., 95: 1423.
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© 1986 Plenum Press, New York
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Hua Hu, D., Kimura, S., Suzuki, T., Maruyama, K. (1986). Isolation of a Native Connectin-Like Protein from the Plasmodium of Physarum polycephalum and its Interaction with Myosin and Actin. In: Dove, W.F., Dee, J., Hatano, S., Haugli, F.B., Wohlfarth-Bottermann, KE. (eds) The Molecular Biology of Physarum polycephalum . NATO ASI Series, vol 106. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2203-0_16
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DOI: https://doi.org/10.1007/978-1-4613-2203-0_16
Publisher Name: Springer, Boston, MA
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