Amyloidosis pp 57-60 | Cite as

Characterization of Two Distinct Serum Amyloid A Gene Products Defined by Their Complementary DNAs

  • J. D. Sipe
  • P. Woo
  • G. Goldberger
  • A. S. Cohen
  • A. S. Whitehead


Two distinct serum amyloid A specific complementary DNA clones have been isolated from a human adult liver library. One clone contains coding information for the alpha form of apoSAA1. The other shares substantial sequence homology, but differs, however, in that it lacks a Pst I restriction site present in its homologue. Both cDNA clones hybridize to an SAA genomic clone.


Southern Blot Hybridization Entire Code Sequence Restriction Enzyme Recognition Site Derive Amino Acid Sequence Alpha Form 
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  1. 1.
    E. P. Benditt, J. S. Hoffman, N. Eriksen, and K. A. Walsh, Ann. N. Y. Acad. Sci., 389: 183 (1982).PubMedCrossRefGoogle Scholar
  2. 2.
    L. L. Bausserman, A. L. Saritelli, P. N. Herbert, K. P. W. J. McAdam, and R. S. Shulman, Biochem. Biophys. Acta, 702: 556 (1982).Google Scholar
  3. 3.
    D. C. Parmelee, K. Titani, L. H. Ericsson, N. Eriksen, E. P. Benditt, and K. A. Walsh, Biochemistry, 21: 3298 (1982).PubMedCrossRefGoogle Scholar
  4. 4.
    J. D. Sipe, A. S. Whitehead, G. Goldberberger, P. Woo, M. D. Edge, B. F. Tack, R. M. Kay, A. S. Cohen, and H. R. Colten, Biochemistry, in press (1985).Google Scholar
  5. 5.
    D. B. Clewell and D. R. Helsinki, Proc. Natl. Acad. Sci., 69: 1159 (1969).CrossRefGoogle Scholar
  6. 6.
    A. Maxara and W. Gilbert, Proc. Natl. Acad. Sci., 74: 560 (1977).CrossRefGoogle Scholar
  7. 7.
    F. Sanger, S. Nicken, and A. R. Coulson, Proc. Natl. Acad. Sci., 74: 5463 (1977).PubMedCrossRefGoogle Scholar
  8. 8.
    M. D. Benton and R. W. Davis, Science, 196: 180 (1977).PubMedCrossRefGoogle Scholar
  9. 9.
    E. M. Southern, J. Mol. Biol., 98: 503 (1975).PubMedCrossRefGoogle Scholar
  10. 10.
    A. J. Jeffreys and R. A. Flavell, Cell, 12: 429 (1977).PubMedCrossRefGoogle Scholar
  11. 11.
    K. Sletten, G. Marhaug, and G. Husby, Hoppe-Seylels Z. Physiol. Chem. Bd., 364: 1039 (1983).CrossRefGoogle Scholar
  12. 12.
    M. A. Hermodson, R. W. Kuhn, K. A. Walsh, H. Neurath, N. Eriksen, and E. P. Benditt, Biochemistry, 11: 2934 (1972).PubMedCrossRefGoogle Scholar
  13. 13.
    M. Skinner, E. S. Cathcart, A. S. Cohen, and M. D. Benson, J. Exp. Med., 140: 871 (1974).PubMedCrossRefGoogle Scholar
  14. 14.
    P. D. Gorevich, Y. Levo, B. Frangione, and E. C. Franklin, J. Immunol., 121: 131 (1978).Google Scholar
  15. 15.
    J. S. Hoffman, L. H. Ericsson, N. Eriksen, K. A. Walsh, and E. P. Benditt, J. Exp. Med., 159: 641 (1984).PubMedCrossRefGoogle Scholar
  16. 16.
    K. Waalen, K. Sletten, G. Husby, and K. Nordstoga, Eur. J. Biochem., 104: 407 (1980).PubMedCrossRefGoogle Scholar
  17. 17.
    P. D. Gorevic, M. Greenwald, B. Frangione, M. Pras, and E. C. Franklin, J. Immunol., 118: 1113 (1977).PubMedGoogle Scholar
  18. 18.
    J. F. Morrow, R. S. Stearman, C. G. Peltzman, and D. A. Potter, Proc. Natl. Acad. Sci., 78: 4718 (1981).PubMedCrossRefGoogle Scholar
  19. 19.
    R. S. Stearman, C. A. Lowell, W. R. Pearson, and J. F. Morrow, Ann. N. Y. Acad. Sci., 389: 106 (1982).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • J. D. Sipe
    • 1
  • P. Woo
    • 2
    • 3
  • G. Goldberger
    • 2
    • 3
  • A. S. Cohen
    • 1
  • A. S. Whitehead
    • 2
    • 3
  1. 1.Arthritis CenterBoston University School of MedicineBostonUSA
  2. 2.Division of Cell BiologyChildrens HospitalUSA
  3. 3.Department of PediatricsHarvard Medical SchoolBostonUSA

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