Abstract
AL proteins, six of the k-type and ten of the λ-type, were isolated from amyloid fibrils obtained from amyloid-laden organs of patients with plasma cell dyscrasias. The AL proteins were subgroups in VkI, VkIII, VλI, VλII, VλIII, VλIV and VλVI. The molecular weight ranged from 12 to 22 kD. At least 4 of the k-type and 5 of the λ-type AL proteins contained carbohydrate. Elucidation of the primary structure of three AL-chains sub-grouped in VλI, VλII and VλIII, revealed several positions where some unique amino acid interchanges had occurred. A comparison of AL-chains and Bence Jones proteins from patients without amyloidosis, indicated that the homology between two AL-chains of the same subgroup was less than that between an AL-chain and a Bence Jones protein. Location of oligosaccharide chains were established by amino acid sequence analyses of glycopeptides. The glycosylation sites were located in the CDR 1, CDR 3 and the FR 4.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
G. G. Glenner, W. D. Terry, and C. Isersky, Semin. Hematol. JLO, (1973). G. Husby, Ann. Clin. Res. 154 (1975).
E. P. Benditt, A. S. Cohen, P. P. Costa, E. C. Franklin, G. G. Glenner, G. Husby, E. Mandema, J. B. Natvig, E. F. Osserman, E. Sohar O. Wegelius and P. Westermark, Amyloid and Amyloidosis. G. G. Glenner, P. P. Costa, A. F. Freitas, Eds. ( Excerpta Medica, Amsterdam 1980 ) p. X I.
J. B. Natvig, P. Westermark, K. Sletten, G. Husby, and T. Michaelsen. Scand. J. Immunol. 14, 89 (1981).
G. G. Glenner, New Eng. J. Med. 302, 1283 and 1333 (1980).
K. Sletten, G. Husby, and J. B. Natvig, Scand. J. Immunol. 3., 8–3 (1974)
P. Westermark, K. Sletten, P. Pitkänen, J. B. Natvig, and C. E. Lindholm, Molec. Immunol. 15, 447 (1982)
M. Pras, M. Schubert, D. Zucker-Franklin, A. Rimon, and E. C. Franklin, J. Clin. Invest. 47, 924 (1968).
R. T. Swank and K. D. Mundres, Anal. Biochem. 39., 462 (1971).
K. Sletten, J. B. Natvig, G. Husby, and J. Juul, Biochem. J. 195, 561 (1981).
C. H. Bolton, J. R. Clamyr and L. Hough, Biochem. J. 96, 5c (1965).
K. Sletten and G. Husby, Eur. J. Biochem. 41, 117 (1974)
I. Kawasaki and H. A. Itano, Anal. Biochem. 48, 546 (1972).
A. Fontana, Methods Enzymol. 25, 419 (1972).
K. Sletten, G. Marhaug, and G. Husby, Hoppe-Seyler1s Z. Physiol. Chem 364, 1039 (1983).
N. Takahashi, T. Takayasu, T. Shinoda, S. Ito, T. Okuyama, and A. Shimizu, Biomed. Res. 1, 321 (1980).
B. Langer, M. Steinmetz-Kayne, and N. Hilschmann, Hoppe-Seyler1s Z. Physiol. Chem. 349, 945 (1968).
H. Ponstingl and N. Hilschmann, Hoppe-Seyler1s Z. Physiol. Chem., 352, 859 (1971).
K. Baczko, D. G. Braun, and N. Hilschmann, Hoppe-SeylerTs Z. Physiol. Chem. 355, 131 (1974).
E. A. Kabat, T. T. Wu, H. Bilofsky, M. Reid-Miller, and H. Perry, Sequences of Proteins of Immunological Interest. U.S. Department of Health and Human Services (1983).
F. A. Garver, L. S. Chang, C. R. Kiefer, J. Mendicino, E. W. Chandrasekaran, T. Isobe, and E. F. Ossermand, Eur. J. Biochem., 115, 643 (1981).
H. C. Sox and L. Hood, Proc. Natl. Acad. Sci., U.S.A. 66, 975 (1970).
G. Sawidou, M. Klein, C. Home, T. Hofmann, and K. F. Dorrington, Molec. Immunol. 18, 793 (1981).
H. Schachter, Clin. Biochem. 17, 3 (1984).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Sletten, K., Westermark, P., Husby, G. (1986). Structural Studies of the Variable Region of Immunoglobulin Light-Chain-Type Amyloid Fibril Proteins. In: Glenner, G.G., Osserman, E.F., Benditt, E.P., Calkins, E., Cohen, A.S., Zucker-Franklin, D. (eds) Amyloidosis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2199-6_59
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2199-6_59
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9292-0
Online ISBN: 978-1-4613-2199-6
eBook Packages: Springer Book Archive