Abstract
The fluorescent and 19F NMR calcium indicators Quin-2 (Tsien et al., 1982) and 5FBAPTA (Smith et al., 1983) have been successfully applied to the measurement of free, cytosolic calcium concentration in a range of animal cells. The indicator is ‘loaded’ into the cytosol by the intra-cellular hydrolysis of a permeant tetraacetoxymethyl ester (Tsien et al., 1982). Thus ease of intracellular localization and their high calcium sensitivity and selectivity (Tsien, 1980) would make such indicators ideally suited for use in plant systems. However, Quin-2 ester is hydrolysed extracellularly by plant cells (unpublished data), perhaps due to esterase activity in the cell wall. So the use of these indicators with the wall-less, mesophyll protoplasts of Nicotiana tabacum L. Cv. Wisconsin 38 was investigated.
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Hesketh, T. R., Smith, G. A., Moore, J. P., Taylor, M.V. and Metcalfe, J. C., 1983, Free cytoplasmic calcium concentration and the mitogenic stimulation of lymphocytes, J. Biol. Chem., 258: 4876
Tsien, R. Y., 1980, New calcium indicators and buffers with high selectivity against magnesium and protons. Design, synthesis and properties of prototype structures, Biochemistry, 19: 2396
Tsien, R. Y., Pozzan, T. and Rink, T. J., 1982, Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium measured with a new, intracellularly trapped fluorescent indicator, J. Cell Biol., 94: 325
Smith, G. A., Hesketh, R. T., Metcalfe, J. C., Feeney, J. and Morris, P. G., 1983, Intracellular calcium measurements by lF NMR of fluorine-labelled chelators, Proc. Natl. Acad. Sci. USA, 80: 7178
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© 1986 Plenum Press, New York
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Gilroy, S., Hughes, W., Trewavas, A.J. (1986). Some Practical Aspects of the Application of Quin-2 to Plant Systems. In: Trewavas, A.J. (eds) Molecular and Cellular Aspects of Calcium in Plant Development. NATO ASI Series, vol 104. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2177-4_66
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DOI: https://doi.org/10.1007/978-1-4613-2177-4_66
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