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The Control of α-Amylase Synthesis by Calcium in the Barley Aleurone

  • Jill Deikman
  • Russell L. Jones
Part of the NATO ASI Series book series (volume 104)

Abstract

Barley aleurone layers synthesize and secrete at least four isozymes of α-amylase which are separated by various criteria (including isoelectric points) into two groups: A (low pI; isozymes 1 and 2) and B (high pI; isozymes 3 and 4). Isozyme 2 is produced under all conditions of aleurone incubation but its rate of accumulation is enhanced by the presence of gibberellic acid (GA). Isozyme 1 requires GA for its production as do isozymes 3 and 4. Isozymes 3 and 4 also require Ca2+ for their accumulation in and secretion from aleurone layers; the production and secretion of isozymes 1 and 2 is completely independent of the level of Ca2+ in the external medium. The dependence of amylase secretion on Ca2+ has been investigated previously and shown to be specific to the B group (Jones, R. L. and Jacobsen, J. V., 1982, Planta 156:421–432), and the Ca2+ requirement was demonstrated to be at the level of the plasma membrane (Moll, B. A. and Jones, R. L., 1982, Plant Physiology 70:1149–1155). The work presented here concerns the effect of Ca2+on amylase synthesis.

Keywords

Isoelectric Point Gibberellic Acid External Medium Aleurone Layer Amylase Secretion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Jill Deikman
    • 1
  • Russell L. Jones
    • 1
  1. 1.Department of BotanyUniversity of CaliforniaBerkeleyUSA

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