Abstract
Hematoporphyrin derivative (Hpd), prepared as described by Lipson et al. is the most widely used sensitizer in photodynamic cancer therapy (PDT). Hpd contains a number of porphyrins, some of which contribute minimally to its photodynamic action2. A somewhat purified version of Hpd, so-called photofrin II (PII), is also used in animal- and in clinical experiments3,4. PII is probably mainly composed of porphyrin dimers and/or oligomers linked together with ether- or ester- bonds, together with a variable proportion of hematoporphyrin (Hp), hydroxyethyl-vinyl-deuteroporphyrin (Hvd) and protoporphyrin (Pp). Porphyrins witji low activity may be removed from Hpd by means of gel chromatography5, dialysis or ultra-centrifugation. Below is presented a simple extraction procedure that leads to a product with properties similar to those of PII.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
R. Lipson, The use of a derivative of hematoporphyrin in tumor detection, J. Natl. Cancer Inst., 26: 1 (1961).
J. Moan, T. Christensen and S. Sommer, The main photosensitizing components of hematoporphyrin derivative, Cancer Lett., 15: 161 (1982).
V.O. Nseyo, T.J. Dougherty, D. Boyle, W. Potter, L.S. Englander, R.P. Huben, and J.E. Pontes, Experimental photodynamic treatment of canine bladder, J. Urol., 133: 311 (1985).
V.O. Nseyo, T.J. Dougherty, D. Boyle, W. Potter, L.S. Englander, R.P. Huben, and J.E. Pontes, Experimental photodynamic treatment of canine bladder, J. Urol., 133: 311 (1985).
S. Sommer, J. Moan, T. Christensen and J.F. Evensen, A chromatographic study of hematoporphyrin derivatives, in: “Porphyrins in Tumor Phototherapy”, A. Andreoni and R. Cubeddu, eds., Plenum Press, New York and London (1984).
J. Moan, B. Høvik and S. Sommer, A device to determine fluence-response curves for photoinactivation of cells in vitro. Photobiochem. Photobiophys., 8: 11 (1984).
J. Moan and S. Sommer, Uptake of the components of hematoporphyrin derivative by cells and tumors. Cancer Lett., 21: 167 (1983).
M.B. Lowe, J.N. Phillips, Metalloporphyrin formation: A possible enzymic model, Nature, 190: 262 (1961).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Plenum Press, New York
About this chapter
Cite this chapter
Sommer, S., Moan, J., Rimington, C. (1985). Separation of Different Fractions of Hematoporphyrin Derivative by a Two-Phase Extraction System. In: Kessel, D. (eds) Methods in Porphyrin Photosensitization. Advances in Experimental Medicine and Biology, vol 193. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2165-1_23
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2165-1_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9276-0
Online ISBN: 978-1-4613-2165-1
eBook Packages: Springer Book Archive