Quantitative Autoradiography of Drug Binding Sites: Methodological Considerations
The goal of this current line of work was to develop quantitative autoradiographic methods to regionally quantify the binding properties (i.e. Kd and Bmax) of opiate ligands in morphine dependent and naive rat brain. Such regional studies were not possible utilizing binding techniques to brain membrane preparations because of the limited availability of tissue from dissection of small brain regions. Even with regional dissection techniques, the fine anatomical resolution available with autoradiography was sacrificed. With quantitative autoradiographic methods, one could ask whether the development of opiate tolerance/dependence occurred as a result of regulation of the affinity or concentration of opiate binding sites in any of one or several regions rich in opiate binding sites.
KeywordsCovariance Chloroform Morphine Methacrylate Tritium
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