Effect of Low Frequency Pulsing Electromagnetic Fields on the Response of Human Normal and Leukemic Lymphocytes to the Lectins
Low frequency Pulsing Electromagnetic Fields (PEMF) are widely utilized to promote congenital and acquired pseudoarthrosis healing in several orthopaedic departments (1,2). In spite of the large use of this kind of treatment for clinical purposes very little is known about the mechanisms of action of PEMF at the cellular level. In vitro studies (3,4) and computerized mathematical models (5) suggest that the biological target at the cellular level of PEMF is the cellular membrane. Specifically those events appointed to the control of Ca++ fluxes are involved (6). Because of the Extremely Low Frequencies (ELF) used, the inside of the cell is shielded in the respect of the electric field associated with PEMF (7). To obtain lymphocyte activation by lectins one of the fundamental events is represented by Ca++ influx across the cellular membrane immediately after the lectin-membrane receptor binding (8). The Ca++influx inhibition completely prevents the lymphocyte activation (fig. 1). For these reasons the response of lymphocytes to the lectins represents a good experimental model to study the mechanisms of action of PEMF. Similarly it is interesting to test the PEMF effect on the naturally impaired response of Chronic Lymphocytic Leukemia (CLL) lymphocytes to the lectins (9): Phytohaemagglutinin (PHA) and Pockweed Mitogen.
KeywordsLeukemia Verapamil Thymidine Rosen
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