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Microvascularization of the alimentary canal as studied by scanning electron microscopy of corrosion casts

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Part of the book series: Electron Microscopy in Biology and Medicine ((EMBM,volume 4))

Abstract

Scanning electron microscopy (SEM) of corrosion casts allows a clearer three-dimensional view of fine vascular architecture than conventional light or transmission electron microscopy of sectioned or injected tissue specimens (1–8). Positional relations of each vessel to the tissue elements cannot be examined in the cast samples, since the cast itself is observed in isolation from the surrounding tissues. However, SEM of vascular corrosion casts has already shown itself to be valuable and useful in the study of microvascular architecture in various organs and tissues, including the gastrointestinal canal (9–24).

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References

  1. Murakami T: Application of the scanning electron microscope to the study of the fine distribution of the blood vessels. Arch Histol Jpn 32: 445–454, 1971.

    PubMed  CAS  Google Scholar 

  2. Nowell JA, Lohse CL: Injection replication of the microvasculature for SEM. In: Scanning Electron Microscopy/1974-I, O Johari, I Corvin (eds), Chicago: IIT Research Institute, p 267–274, 1974.

    Google Scholar 

  3. Murakami, T: Methyl methacrylate injection replica method. In: Principles and Techniques of Scanning Electron Microscopy, vol. 6. MA Hayat (ed), New York: Van Nostrand Reinhold Co., p 159–169, 1978.

    Google Scholar 

  4. Gannon, BJ: Vascular casting. In: Principles and Techniques of Scanning Electron Microscopy, vol 6. MA Hayat (ed), New York: Van Nostrand Reinhold Co., p 170–193, 1978.

    Google Scholar 

  5. Frasca JM, Carter HW, Schaffer WA: An improved latex injection media for replicating the pulmonary microvasculature. In: Scanning Electron Microscopy/1978/II. O Johari (ed), Chicago: AMF O’Hare, SEM Inc, p 485–489, 1978.

    Google Scholar 

  6. Hodde KC, Nowell JA: SEM of microcorrosion casts. In: Scanning Electron Microscopy/1980/11. RP Becker, O Johari (eds), Chicago: AMF O’Hare, SEM Inc, p 88–106, 1980.

    Google Scholar 

  7. Murakami T, Ohtani O, Ohtsuka A, Kikuta A: Injection replication and scanning electron microscopy of blood vessels. In: Biomedical Research Applications of Scanning Electron Microscopy, Vol 3. GM Hodges, KE Carr (eds), London: Academic Press, p 1–30, 1983.

    Google Scholar 

  8. Lametschwandtner A, Lametschwandtner U, Weiger T: Scanning electron microscopy of vascular corrosion casts - technique and applications. In: Scanning Electron Microscopy/1984/II. O Johari (ed), Chicago: AMF O’Hare, SEM Inc, p 663–695, 1984.

    Google Scholar 

  9. Ohashi Y, Kita S, Murakami T: Microcirculation of the rat small intestine as studied by the injection replica scanning electron microscope method. Arch Histol Jpn 39: 271–282, 1976.

    PubMed  CAS  Google Scholar 

  10. Kobayashi A, Takeda S, Hattori S, Kohli Y, Hattori T, Kawai K: Vascular architecture of human gastric mucosa. Jpn J Gastroenterol 73: 169–178, 1976 (in Japanese).

    CAS  Google Scholar 

  11. Takao, M: Microcirculation of gastric ulcer. A scanning electron microscopic study of vascular casts. Okayama Igakkai Z 90: 915–948, 1978 (in Japanese).

    Google Scholar 

  12. Nopanitaya W, Aghajanian JG, Gray LD: An improved plastic mixture for corrosion casting of the gastrointestinal microvascular system. In: Scanning Electron Microscopy/ 1979/111. RP Becker, O Johari (eds), Chicago: AMF O’Hare, SEM Inc, p 751–755, 1979.

    Google Scholar 

  13. Tamura A: Scanning electron microscopic studies on lymphatic casts of the rat stomach and the rabbit small intestine. Okayama Igakkai Z 91: 1075–1099, 1979 (in Japanese).

    Google Scholar 

  14. Gannon BJ, Rogers PAW, O’Brien PE: Two capillary plexuses in human intestinal villi. Micron 11: 447–448, 1980.

    Google Scholar 

  15. Bhalla DK, Murakami T, Owen RL: Microcirculation of intestinal lymphoid follicles in rat Peyer’s patches. Castroenterol 81: 481–491, 1981.

    CAS  Google Scholar 

  16. Gannon BJ, Gore RW, Rogers PAW: Is there an anatomical basis for a vascular countercurrent mechanism in rabbit and human intestinal villi? Biomed Res 2 (suppl): 235–241, 1981.

    Google Scholar 

  17. Gannon B, Browning J, O’Brien P: The microvascular architecture of the glandular mucosa of rat stomach. J Anat 135: 667–683, 1982.

    PubMed  CAS  Google Scholar 

  18. Métry JM, Neff M, Knoblauch M: The microcirculatory system of the intestinal mucosa of the rat. An injection cast and scanning electron microscopy study. Scand J Gastroenterol 17 (suppl 71): 159–162, 1982.

    Google Scholar 

  19. Bockman DE: Functional histology of appendix. Arch Histol Jpn 46: 271–292, 1983.

    Article  PubMed  CAS  Google Scholar 

  20. Ohtani O, Kikuta A, Ohtsuka A, Taguchi T, Murakami T: Microvasculature as studied by the microvascular corrosion casting/scanning electron microscope method. I. Endocrine and digestive system. Arch Histol Jpn 46: 1–42, 1983.

    Article  CAS  Google Scholar 

  21. Yamaguchi K, Schoefl GI: Blood vessels of the Peyer’s patch in the mouse: I. Topographic studies. Anat Rec 206: 391–401. 1983.

    Article  PubMed  CAS  Google Scholar 

  22. Gannon B, Browning J, O’Brien P, Rogers P: Mucosal microvascular architecture of the fundus and body of human stomach. Gastroenterol 86: 866–875, 1984.

    CAS  Google Scholar 

  23. Ohtsuka A, Ohtani O: The microvascular architecture of the rabbit stomach corpus in vascular corrosion casts. In: Scanning Electron Microscopy /1984/IV. O Johari (ed), Chicago: AMF O’Hare, SEM Inc, p 1951–1956, 1984.

    Google Scholar 

  24. Ohtani O, Ohtsuka A: Three-dimensional organization of lymphatics and their relationship to blood vessels in rabbit small intestine. A scanning electron microscopic study of corrosion casts. Arch Histol Jpn 48: 255–268, 1985.

    Article  PubMed  CAS  Google Scholar 

  25. Murakami T, Itoshima T, Hitomi K, Ohtsuka A, Jones AL: A monomeric methyl and hydroxypropyl methacrylate injection medium and its utility in casting blood capillaries and bile canaliculi for scanning electron microscopy. Arch Histol Jpn 47: 223–237, 1984.

    Article  PubMed  CAS  Google Scholar 

  26. Barlow TE, Bentley FH, Walder DN: Arteries, veins, and arteriovenous anastomoses in the human stomach. Surg Gynecol Obstet 93: 657–671, 1951.

    PubMed  CAS  Google Scholar 

  27. Oka S: Studies on the microcirculation of gastrointestinal mucosa. Saishin Igaku 25: 1705–1713, 1970 (in Japanese).

    PubMed  CAS  Google Scholar 

  28. Hase T, Moss BJ: Microvascular changes of gastric mucosa in the development of stress ulcer in rats. Gastroenterology 65: 224–234, 1973.

    PubMed  CAS  Google Scholar 

  29. Guth PH, Rosenberg A: In vivo microscopy of the gastric microcirculation. Am J Digest Dis 17: 391–398, 1972.

    Article  PubMed  CAS  Google Scholar 

  30. Piasecki C: Blood supply to the human gastroduodenal mucosa with special reference to the ulcer-bearing areas. J Anat 118: 295–335, 1974.

    PubMed  CAS  Google Scholar 

  31. Piasecki C: Observations on the submucous plexus and mucosal arteries of the dog’s stomach and first part of the duodenum. J Anat 119: 133–148, 1975.

    PubMed  CAS  Google Scholar 

  32. Fujita H, Fujita T: Textbook of histology, part 2, 2nd ed. Tokyo: Igaku-Shoin, 1984 (in Japanese).

    Google Scholar 

  33. Johansson C, Aly A: Stimulation of gastric mucus output by somatostatin in man. Eur J Clin Invest 12: 37–39, 1982.

    Article  PubMed  CAS  Google Scholar 

  34. Kusumoto Y, Iwanaga T, Ito S, Fujita T: Juxtaposition of somatostatin cell and parietal cell in the dog stomach. Arch Histol Jpn 42: 459–465, 1979.

    PubMed  CAS  Google Scholar 

  35. Browning J, Gannon B: The microvascular architecture of rat proximal duodenum, with particular reference to Brunner’s glands. Biomed Res 5: 245–258, 1984.

    Google Scholar 

  36. Mall JP: Die Blut- und Lymphwege im Dfinndarm des hundes. Abh Mat-Phys I Konig Sachs Gesell Wiss 14: 153–189, 1887.

    Google Scholar 

  37. Spanner R: Neue Befunde über die Blutwege der Darmwand und ihre funktionelle Bedeutung. Morphol Jahrb 69: 394–454, 1932.

    Google Scholar 

  38. Jacobson LF, Noer RJ: The vascular pattern of the intestinal villi in various laboratory animals and man. Anat Rec 114: 85–101, 1952.

    Article  PubMed  CAS  Google Scholar 

  39. Nylander G, Olerud S: The vascular pattern of an isolated jejunal loop. A microangiographic study in the rat. Acta Chir Scand 121: 39–46, 1961.

    PubMed  CAS  Google Scholar 

  40. Mohiuddin A: Blood and lymph vessels in the jejunal villi of the white rat. Anat Rec 156: 83–90, 1966.

    Article  PubMed  CAS  Google Scholar 

  41. Reynolds DG, Brim J, Sheehy TW: The vascular architecture of the small intestinal mucosa of the monkey (Macaca mulatta). Anal Rec 159: 211–218, 1967.

    Article  CAS  Google Scholar 

  42. Miller DS, Rahman MA, Tanner R, Mathan VI, Baker SJ: The vascular architecture of the different forms of small intestinal villi in the rat (Rattus norvegicus). Scand J Gastroenterol 4: 477–482, 1969.

    Article  PubMed  CAS  Google Scholar 

  43. Reynolds DG, Swan KG: Intestinal microvascular architecture in endotoxic shock. Gastroenterol 63: 601–610, 1972.

    CAS  Google Scholar 

  44. Bellamy JEC, Latshaw WK, Nielsen NO: The vascular architecture of the porcine small intestine. Can J Comp Med 37: 56–62, 1973.

    PubMed  CAS  Google Scholar 

  45. Svanvik J: Mucosal blood circulation and its influence on passive absorption in the small intestine. An experimental study in the cat. Acta Physiol Scand (suppl) 385: 1–44, 1973.

    CAS  Google Scholar 

  46. Winne D: The vasculature of the jejunal villus. Excerpta Medica International Congress Series 391: 56–57, 1973.

    Google Scholar 

  47. Szyika AS: Systématisation de l’angioarchitectonie du jéjunum et de l’iléon chez l’homme adulte. Arch Anat Histol Embryol Norm Exp 59: 79–142, 1976.

    CAS  Google Scholar 

  48. Wolfram-Gabel R: Systématisation de l’angioarchitectonic du jéjunum et de l’iléon chez l’homme en période périnatale. Arch Anat Histol Embryol Norm Exp 63: 39–142, 1980.

    CAS  Google Scholar 

  49. Wolfram-Gabel R, Maillot Cl: La vascularisation des tuniques sous-muqueuse et muqueuse des première et deuxième portions du duodenum chez l’homme adulte. Arch Anat Histol Embryol Norm Exp 64: 129–171, 1981.

    CAS  Google Scholar 

  50. Eriksson B: Microangiographic pattern in the small intestine of the cat after irradiaton. Scand J Gastroenterol 17: 887–895, 1982.

    Article  PubMed  CAS  Google Scholar 

  51. Dabelow A: Die Blutgefäßversorgung der lymphatischen Organe. Verhand Anat Gesel 46 Versam (Anat Anz 87 (Erg)): 179–224, 1939.

    Google Scholar 

  52. Abe K, Ito T: A qualitative and quantitative morphologic study of Peyer’s patches of the mouse. Arch Histot Jpn 40: 407–420, 1977.

    Google Scholar 

  53. Blau JN: A comparative study of the microcirculation in the guinea pig thymus, lymph nodes and Peyer’s patches. Clin Exp Immunot 27: 340–347, 1977.

    Google Scholar 

  54. Gowans JL, Knight EJ: The route of recirculation of lymphocytes in the rat. Proc R Soc Lond (Biol) 159: 257–282, 1964.

    Article  PubMed  CAS  Google Scholar 

  55. Marchesi VT, Gowans JL: The migration of lymphocytes through the endothelium of venules in lymph nodes: An electron microscope study. Proc R Soc Lond (Biol) 159: 283–290, 1964.

    Article  PubMed  CAS  Google Scholar 

  56. Browning J, Gannon B: Mucosal microvascular organization of the rat colon. Acta Anat 126: 73–77, 1986.

    Article  PubMed  CAS  Google Scholar 

  57. Wolfram-Gabel R, Maillot CI, Koritke JG: La vascularisation des tuniques sous-muqueuse et muqueuse du colon chez l’homme. Arch Anat Histol Embryol Norm Exp 66: 67–98, 1983.

    CAS  Google Scholar 

  58. Owen RL, Nemanic P: Antigen processing structures of the mammalian intestinal tract: An SEM study of lymphoepithelial organs. In: Scanning Electron Microscopy/1978/11 RP Becker, O Johari (eds). Chicago: AMF O’Hare. SEM Inc, p 367–378, 1978.

    Google Scholar 

  59. Shimizu S: Beiträge zur Anatomy des Lymphgefässsystems der Wirbeltiere und des Menschen (Japaner). Nr. 8. Darmzotten und ihre Gefässe, insbesondere die Chylusgefässe der Säugetiere und des Menschen. Okajimas Folia Anat Jpn 10: 193–227, 1932.

    Google Scholar 

  60. Ohtani O: Three-dimensional organization of lymphatics and its relationship to blood vessels in rat small intestine. Cell Tiss Res 248: 365–374, 1987

    Article  CAS  Google Scholar 

  61. Ohtani O, Ohtsuka A, Owen RL: Three-dimensional organization of the lymphatics in the rabbit appendix. A scanning electron and light microscopic study. Gastroenterology 91: 947–955, 1986.

    PubMed  CAS  Google Scholar 

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© 1988 Martinus Nijhoff Publishing, Boston

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Ohtsuka, A., Ohtani, O., Murakami, T. (1988). Microvascularization of the alimentary canal as studied by scanning electron microscopy of corrosion casts. In: Motta, P.M., Fujita, H., Correr, S. (eds) Ultrastructure of the Digestive Tract. Electron Microscopy in Biology and Medicine, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2071-5_12

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  • DOI: https://doi.org/10.1007/978-1-4613-2071-5_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9229-6

  • Online ISBN: 978-1-4613-2071-5

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