Intrarenal Localization and Photoaffinity Labeling of Receptors for α-Rat Atrial Natriuretic Polypeptide
Although it has been well established that atrial natriuretic polypeptide (ANP) causes natriuresis by directly acting on the kidney, the detailed intrarenal distribution and characterization of ANP receptors are unknown. We examined intrarenal localization of receptors for α-rat ANP (α-rANP) in the rat kidney by in vivo (1) or in vitro (2) autoradiography, using 125I-labeled ligand. In order to identify the receptors for α-rANP, [125I]α-rANP was directly cross-linked to its receptors in isolated glomerulus, inner medullary collecting tubule (IMCT) rich fraction, adrenel gland, and aorta by direct UV-photoaffinity labeling (3). The purpose of this communication is to summarize the results of these recently reported studies.
KeywordsOuter Medulla PHOTOAFFINITY Label Percoll Density Gradient Outer Stripe Rich Fraction
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- 1.Koseki, C, Kanai, Y., Hayashi, Y., Ohnuma, N., and Imai, M. Japan. J. Pharmacol, in pressGoogle Scholar