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Intrarenal Localization and Photoaffinity Labeling of Receptors for α-Rat Atrial Natriuretic Polypeptide

  • C. Koseki
  • Y. Hayashi
  • M. Furuya
  • Y. Kanai
  • N. Ohnuma
  • M. Imai
Chapter
Part of the Developments in Nephrology book series (DINE, volume 18)

Abstract

Although it has been well established that atrial natriuretic polypeptide (ANP) causes natriuresis by directly acting on the kidney, the detailed intrarenal distribution and characterization of ANP receptors are unknown. We examined intrarenal localization of receptors for α-rat ANP (α-rANP) in the rat kidney by in vivo (1) or in vitro (2) autoradiography, using 125I-labeled ligand. In order to identify the receptors for α-rANP, [125I]α-rANP was directly cross-linked to its receptors in isolated glomerulus, inner medullary collecting tubule (IMCT) rich fraction, adrenel gland, and aorta by direct UV-photoaffinity labeling (3). The purpose of this communication is to summarize the results of these recently reported studies.

Keywords

Outer Medulla PHOTOAFFINITY Label Percoll Density Gradient Outer Stripe Rich Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Koseki, C, Kanai, Y., Hayashi, Y., Ohnuma, N., and Imai, M. Japan. J. Pharmacol, in pressGoogle Scholar
  2. 2.
    Koseki, C, Hayashi, Y., Torikai, S., Furuya, M., Ohnuma, N., and Imai, M. Am. J. Physiol. 250 (Renal Fluid Electrolite Physiol. 19): F210–F216, 1986PubMedGoogle Scholar
  3. 3.
    Koseki, C, Hayashi, Y., Ohnuma, N., and Imai, M. Biochem. Biophys. Res. Commun. 136: 200–207, 1986PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1987

Authors and Affiliations

  • C. Koseki
    • 1
    • 2
  • Y. Hayashi
    • 1
    • 2
  • M. Furuya
    • 1
    • 2
  • Y. Kanai
    • 1
    • 2
  • N. Ohnuma
    • 1
    • 2
  • M. Imai
    • 1
    • 2
  1. 1.Department of PharmacologyNational Cardiovascular Center Research InstitutetOsakaJapan
  2. 2.Department of Clinical PharmacologySuntory Institute for Biomedical ResearchOsakaJapan

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