Characteristics of Ca2+/Mg2+ Atpase in Heart Sarcolemma Treated with Trypsin

  • M. B. Anand-Srivastava
  • N. S. Dhalla
Part of the Developments in Cardiovascular Medicine book series (DICM, volume 66)


Both biochemical and histochemical techniques have demonstrated the presence of an ATP- hydrolyzing enzyme in heart sarcolemma, which is activated by millimolar concentrations of various divalent cations including Ca2+ or Mg2+ (1–5). Earlier studies have revealed that the activity of this sarcolemmal Ca2+/Mg2+ ATPase was decreased by different agents, which have been shown to exert cardiodepressant effects (6–8). Furthermore, interventions, which are known to increase cardiac contractile force, were shown to increase the sarcolemmal Ca2+/Mg2+ ATPase activity (9,10). The activation of this enzyme system was found to exhibit a closed relationship with the increase in cardiac contractile force at different concentrations of Ca 2+ (11). The activities of sarcolemmal Ca 2+/Mg 2+ ATPase have been shown to increased under pathological situations which favour the occurrence of intracellular Ca2+ overload (13,14) but were depressed in other types of failing hearts (15,16). These observations support the view that Ca2+/Mg2+ ATPase may be involved in Ca2+-gating mechanisms and thus may play an important role in regulating the contractile force development in the cardiac muscle (11,16).


Membrane Protein ATPase Activity Trypsin Treatment High Affinity Site Osmium Tetraoxide 
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  1. 1.
    McNamara, D.B., Singh, J.N. and Dhalla, N.S. J, Biochem. 76:603–609, 1974.Google Scholar
  2. 2.
    Dhalla, N.S, Anand, M.B, and Harrow, J.A.C, J, Biochem. 79:1345–1350, 1976.Google Scholar
  3. 3.
    Anand, M.B., Chauhan, M.S. and Dhalla, N.S. J, Biochem. 82:1731–1739, 1977.Google Scholar
  4. 4.
    Anand-Srivastava, M.B, Panagla, V. and Dhalla, N.S. Advances in Myooardiol. 3:359–371, 1982.Google Scholar
  5. 5.
    Malouf, N.N. and Meissner, G. J. Histoohem. Cytochem. 28:1286–1294, 1980.CrossRefGoogle Scholar
  6. 6.
    Dhalla, N.S, Lee, S. L, Anand, M. B., Chauhan, M. S. Biochem, Pharmacol. 26:2055–2060, 1977.CrossRefGoogle Scholar
  7. 7.
    Dhalla, N.S., Harrow, J.A.C. and Anand, M.B. Biochem. Pharmacol. 27:1281–1283, 1978.PubMedCrossRefGoogle Scholar
  8. 8.
    Harrow, J.A.C, Das, P.K. and Dhalla, N.S. Bioohem. Pharmacol. 27: 260–2609, 1978.Google Scholar
  9. 9.
    Ziegelhoffer, A., Anand-Srivastava, M.B., Khandelwal, R.L. and Dhalla, N.S. Biochem. Biophys. Res. Commun. 89 1073–1081, 1979.PubMedCrossRefGoogle Scholar
  10. 10.
    Dhalla, N.S., Ziegelhoffer, A. and Makino, N. In: Pathobiology of Cardiovasoular Injury (Eds. H.L. Stone and W.B. Weglicki), Martinus Nijhoff Publishing, Boston, 1985, pp. 222–231.Google Scholar
  11. 11.
    Dhalla, N.S., Pieroe, G.N, Panagla, V, Singal, P. K, and Beamish, R. E, Basic Res, Cardiol. 77: 117–139, 1982.CrossRefGoogle Scholar
  12. 12.
    Moffat, M.P. and Dhalla, N.S, Can, J. Cardiol. 1: 194–200, 1985.Google Scholar
  13. 13.
    Dhalla, N.S., Smith, C. I, Pieroe, G. N., Elimban, V., Makino, N. and Khatter, J. C. In:The Regulation of Heart Funotion (Ed. H. Rupp), Thieme, New York, 1986, pp. 121–136.Google Scholar
  14. 14.
    Tomlinson, C.W., Lee, S.L. and Dhalla, N.S. Circ. Res. 39:82–92, 1976.PubMedGoogle Scholar
  15. 15.
    Panagla, V., Singh, J.N., Anand-Srivastava, M.B, Pieroe, G.N., Jasmin, G. and Dhalla, N.S. Cardiovaso. Res. 18: 567–572, 1984.CrossRefGoogle Scholar
  16. 16.
    Dhalla, N.S., Ziegelhoffer, A. and Harrow, J.A.C. Can. J. Physiol. Pharmacol. 55: 1211–1234, 1977.PubMedCrossRefGoogle Scholar
  17. 17.
    Panagla, V., Lamers, J.M.J., Singal, P.K. and Dhalla, N.S. Int. J. Biochem. 14: 387–397, 1982.CrossRefGoogle Scholar
  18. 18.
    Dhalla, N.S., Anand-Srivastava, M.B., Tuana, B.S. and Khandelwal, R.L. J. Mol. Cell. Cardiol. 13:413–423, 1981.CrossRefGoogle Scholar
  19. 19.
    Tuana, B.S. and Dhalla, N.S, J, Biol. Chem. 251:14440–14445, 1982.Google Scholar
  20. 20.
    Taussky, H.H. and Shorr, E. J. Biol, Chem, 202: 675–685, 1983.Google Scholar
  21. 21.
    Lowry, O.H., Rosebrough, N. Y, Farr, A. L, and Randall, R. J. J, Biol, Chem, 193:265–275, 1951Google Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1987

Authors and Affiliations

  • M. B. Anand-Srivastava
    • 1
  • N. S. Dhalla
    • 1
  1. 1.Experimental Cardiology Section, Department of PhysiologyUniversity of Manitoba, Faculty of MedicineWinnipegCanada

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