Abstract
Autoradiography is a photographic method of recording the spatial distribution of radioisotopes within a particular tissue, cell, cell organelle, or molecule. In this technique, a radioactively labeled specimen, such as a tissue slice or polyacrylamide gel, is placed in direct contact with a photographic emulsion designed for autoradiography. The radioactive atoms will decay in the sample, and emitted radiation will activate individual silver halide grains in the emulsion, rendering them susceptible to conversion into metallic silver by a photographic developer.
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References
Bonner, W. M., and Laskey, R. A., 1974, A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels, Eur. J. Biochem. 46:83–88.
Freifelder, D., 1976, Physical Biochemistry: Applications to Biochemistry and Molecular Biology, W. H. Freeman, San Francisco.
Hahn, E. J., 1983, Autoradiography: A review of basic principles. Am. Lab. 15:64–71.
Laskey, R. A., and Mills, A. D., 1975, Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography, Eur. J. Biochem. 56:335–341.
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© 1987 Plenum Press, New York
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Dunbar, B.S. (1987). Use of Autoradiography in Polyacrylamide Gel Electrophoresis. In: Two-Dimensional Electrophoresis and Immunological Techniques. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1957-3_7
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DOI: https://doi.org/10.1007/978-1-4613-1957-3_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-42839-5
Online ISBN: 978-1-4613-1957-3
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