Protein Detection in Polyacrylamide Gel Electrophoresis
An investigator must take several factors into account for the process of staining and recording the location of bands after electrophoresis (Wilson, 1979, 1983). These should include (1) the selection and availability of an appropriate dye of satisfactory quality that will give adequate sensitivity; (2) the selection of a solvent that will provide a suitable dye concentration, solubility, and stability; (3) the formation of a stable dye—protein complex; (4) the easy removal of excess free dye for easy detection of stained proteins; and (5) storage of gels for further reference or for permanent recording by photography or analysis by computer, densitometry, or spectrophotometry.
KeywordsSurfactant Hydroxide Agarose Electrophoresis Polypeptide
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