Pulsed and Resting Cytochrome Oxidase: An Update of Optical and Molecular Properties

  • Maurizio Brunori
  • Paolo Sarti
  • Francesco Malatesta
  • Giovanni Antonini
  • Michael T. Wilson

Abstract

The name “pulsed” oxidase was initially assigned to the transient species observed upon exposure of reduced cytochrome c oxidase, in the presence of cytochrome c and excess ascorbate, to a pulse of dioxygen (1). The pulsed species obtained according to this procedure was found to be several times more efficient, in terms of turnover number, than the resting enzyme i.e. the oxidized enzyme as obtained from the purification procedure (2).

Keywords

Peroxide Sucrose Recombination Catalysis Adduct 

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Maurizio Brunori
    • 1
    • 2
    • 3
  • Paolo Sarti
    • 1
    • 2
    • 3
  • Francesco Malatesta
    • 1
    • 2
    • 3
  • Giovanni Antonini
    • 1
    • 2
    • 3
  • Michael T. Wilson
    • 1
    • 2
    • 3
  1. 1.Department of Biochemical Sciences and CNR Center of Molecular BiologyUniversity of Rome “La Sapienza”Italy
  2. 2.Department of Experimental Medicine and Biochemical SciencesUniversity of Rome “Tor Vergata”RomeItaly
  3. 3.Department of ChemistryUniversity of EssexColchesterUK

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