Antigens Expressed by Melanoma and Melanocytes
Serological studies with human sera and monoclonal antibodies have identified a large number of distinct antigen systems on melanoma cells. A major focus of the mapping of these antigens has been the identification of determinants that can serve as targets for recognition by the host. In the course of these studies, more has been revealed about the antigenic profile of normal cells than about specific antigenic changes that occur during the process of malignant transformation. The cell components that have been best characterized are differentiation antigens. The investigation of these antigens on melanoma cells has provided considerable information about antigenic changes occurring during normal differentiation of the normal cell counterpart, the melanocyte. The search continues for molecules that are expressed specifically during events of malignant transformation and tumor progression. Three categories of antigens on melanoma cells have been identified: (1) differentiation antigens; (2) antigens expressed during transformation; and (3) antigens that serve as targets for the immune system in the host with cancer. In this chapter, we discuss our recent studies, rather than review the substantial amount of information that has been published about melanoma antigens.
KeywordsMelanoma Cell Melanoma Cell Line Human Melanoma Cell Helper Virus Human Melanocyte
Unable to display preview. Download preview PDF.
- 4.Real, F. X., Houghton, A. N., Albino, A. P., Cordon-Cardo, C., Melamed, M. R., Oettgen, H. F., and Old, L. J., 1985, Surface antigens of melanomas and melanocytes defined by mouse monoclonal antibodies: Specificity analysis and comparison of antigen expression in cultured cells and tissues, Cancer Res. 45:4401–4411.PubMedGoogle Scholar
- 9.Vogel, C.-W., Welt, S., Carswell, E., Old, L. J., and Müller-Eberhard, H. J., 1983, A murine IgG3 monoclonal antibody to a melanoma antigen that activates complement in vitro and in vivo, Immunobiology 164:309–313.Google Scholar
- 10.Knuth, A., Dippold, W. G., Houghton, A. N., Meyer zum Buschenfelde, K.-H., Oettgen, H. F., and Old, L. J., 1984, ADCC reactivity of human melanoma cells with mouse monoclonal antibodies, Proc. Am. Assoc. Cancer. Res. 25:1005.Google Scholar
- 11.Dippold, W. G., Knuth, A., and Meyer zum Buschenfelde, K.-H., 1983, Inhibition of human melanoma cell growth in vitro by monoclonal anti-GD3 ganglioside antibody, Cancer Res. 44:806–810.Google Scholar
- 14.Houghton, A. N., Mintzer, D., Cordon-Cardo, C., Welt, S., Fliegel, B., Vadhan, S., Carswell, E., Melamed, M., Oettgen, H. F., and Old, L. J., 1985, Mouse monoclonal IgG3 antibody detecting GD3 ganglioside: A phase I trial in patients with malignant melanoma, Proc. Natl. Acad. Sci. USA 82:1242–1246.PubMedCrossRefGoogle Scholar
- 21.Thomson, T., Real, F. X,, Murakami, S., Cordon-Cardo, C., Old, L. J., and Houghton, A. N., 1986, Differentiation antigens of melanocytes and melanoma: Analysis of melanosome and cell surface markers of human pigmented cells with monoclonal antibodies (submitted for publication).Google Scholar
- 28.Rettig, W., Dracopoli, N. C., Goetzger, T. A., Spengler, B. A., Biedler, J. L., Oettgen, H. F., and Old, L. J., 1984, Somatic cell genetic analysis of human cell surface antigens: Chromosomal assignments and regulation of expression in rodent-human hybrid cells, Proc. Natl. Acad. Sci. USA 81:6437–6441.PubMedCrossRefGoogle Scholar
- 32.Albino, A. P., Lloyd, K. O., Houghton, A. N., Oettgen, H. F., and Old, L. J., 1981, Heterogeneity in surface antigen expression and glycoprotein expression of cell lines derived from different metastases of the same patient: Implications for the study of tumor antigens, J. Exp. Med. 154:1764–1778.PubMedCrossRefGoogle Scholar