Polymer Modification by Radiation Grafting in the Presence of Salt Additives and its Application in Enzyme Immobilization

  • J. L. Garnett
  • S. V. Jankiewicz
  • M. A. Long
  • D. F. Sangster
Chapter

Abstract

A novel radiation grafting method for modifying polymers of use in biomedical work is discussed. The procedure involves the use of particular inorganic salts as additives for accelerating the radiation grafting process. Styrene is used as typical monomer in these studies with cellulose, the polyolefins and PVC as representative backbone polymers. Two radiation grafting techniques are examined, namely pre-irradiation and simultaneous methods. The parameters affecting the observed grafting enhancement in the presence of these salt additives are treated. The results are compared with analogous data previously obtained with other additives, including acids. A new model for the mechanism of enhancement in radiation grafting in the presence of acids and/or salts is proposed. A typical biomedical application of the salt grafting technique for enzyme immobilization is briefly discussed.

Keywords

Lithium Polyethylene Acidity Immobilization H2SO4 

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References

  1. 1.
    J. L. Garnett, Radiation Phys. Chem., 14, 79 (1979)Google Scholar
  2. 2.
    B. D. Ratner and A. S. Hoffman, J. Appl. Polym. Sci., 18, 3183 (1974)CrossRefGoogle Scholar
  3. 3.
    V. Stannett, “Graft Copolymerization of Lignocellulose Fibers”, Am. Chem. Soc. Symp. Ser. No. 187, D.N.-S Hon, Ed., An. Chem. Soc. Washington, D. C., 1982, p. 3.Google Scholar
  4. 4.
    J. C. Arthur, Jr., “Graft Copolynerization of Lignocellulose Fibers”, An. Chem. Soc. Symp. Ser. No. 187, D. H. -S. Hon, Ed., An. Chem. Soc., Washington, D. C., 1982, p. 21.Google Scholar
  5. 5.
    J. L. Garnett, S. V. Jankievicz, R. Levot and D. F. Sangster, Radiation Phys. Chem., in press.Google Scholar
  6. 6.
    C. H. Ang, J. L. Garnett, R. Levot and H. A. Long, J. Appl. Polyn. Sci., 27, 4893 (1982).CrossRefGoogle Scholar
  7. 7.
    S. Dilli and J. L. Garnett, Aust. J. Chem., 24, 981 (1971).CrossRefGoogle Scholar
  8. 8.
    B. S. Karten and T. S. Ma, Microchem. J., 3, 507 (1959).CrossRefGoogle Scholar
  9. 9.
    L. Goldstein, Biochem. Biophys. Acta, 315, 1 (1973).Google Scholar
  10. 10.
    S. Blackburn, Anino Acid Deternination, Arnold, London, 1968.Google Scholar
  11. 11.
    J. P. Greenstein and H. Winitz, Chemistry of the Amino Acids, Wiley, New York, 1961.Google Scholar
  12. 12.
    E. W. Yenm and E. C. Cocking, Analyst, 80, 209 (1955).CrossRefGoogle Scholar
  13. 13.
    H. Bergmeyer, Hethods of Enzynatic Analyses, Acadenic Press, New York, 1963.Google Scholar
  14. 14.
    W. J. Chappas and J. Silvernan, Radiat. Phys. Chem., 14, 487 (1979).Google Scholar
  15. 15.
    C. H. Ang, J. L. Garnett, S. V. Jankievicz and D. F. Sangster, “Graft Copolynerization of Lignocellulose Fibers”, An. Chem. Soc. Symp. Ser. No. 187, D. N. -S. Hon, Ed., An. Chem. Soc., Washington, D. C., 1982, p. 141.Google Scholar
  16. 16.
    J. H. Baxendale and F. W. Mellows, J. An. Chem. Soc., 83, 4720 (1962).CrossRefGoogle Scholar
  17. 17.
    A. Ekstrom and J. L. Garnett, J. Phys. Chem., 70, 324 (1966).CrossRefGoogle Scholar
  18. 18.
    J. L. Garnett, S. V. Jankievicz, R. Levot, H. A. Long and D. F. Sangster, Radiat. Phys. Chem., in press.Google Scholar
  19. 19.
    J. L. Garnett, S. V. Jankievicz and D. F. Sangster, unpublished work.Google Scholar
  20. 20.
    S. Dilli and J. L. Garnett, Aust. J. Chem., 21, 397 (1968)CrossRefGoogle Scholar
  21. 21.
    H. Borzaghi, A. Ganba, G. Horosi and H. Sinonetta, J. Phys. Chem., 78, 49 (1974).CrossRefGoogle Scholar
  22. 22.
    D. L. Regan and P. F. Greenfield, Chem. Aust., 45, 317 (1978).Google Scholar
  23. 23.
    S. Venkataranan, T. A. Horbett and A. S. Hoffnan, J. Biomed. Mater. Res. Symp., 8, 111 (1977).CrossRefGoogle Scholar
  24. 24.
    S. Bett and J. L. Garnett, unpublished work.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • J. L. Garnett
    • 1
  • S. V. Jankiewicz
    • 1
  • M. A. Long
    • 1
  • D. F. Sangster
    • 2
  1. 1.School of ChemistryThe University of New South WalesAustralia
  2. 2.Division of Chemical PhysicsCSIROSutherlandAustralia

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